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2.1.1.63: methylated-DNA-[protein]-cysteine S-methyltransferase

This is an abbreviated version!
For detailed information about methylated-DNA-[protein]-cysteine S-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.63

Reaction

DNA (containing 4-O-methylthymine)
+
protein L-cysteine
=
DNA (without 4-O-methylthymine)
 +
protein S-methyl-L-cysteine

Synonyms

Ada protein, Ada-C, AGAT, AGT, AGTendoV, alkylated DNA-protein alkyltransferase, alkylguanine transferase, ATASE, C-hAGT, carboxyl terminal domain of the inducible Escherichia coli ada alkyltransferase, hAGT, methylated-DNA-[protein]-cysteine S-methyltransferase, methylguanine DNA methyltransferase, MGMT, Mgt1, MJ1529, N-hAGT, O6-alkylguanine DNA alkyltransferase, O6-alkylguanine DNA-alkyltransferase, O6-alkylguanine-DNA alkyl-transferase, O6-alkylguanine-DNA alkyltransferase, O6-methylguanine DNA methyltransferase, O6-methylguanine methyltransferase, O6-methylguanine-DNA methyltransferase, O6-methylguanine-DNA-methyltransferase, O6-MGMT, O6meG-DNA alkyltransferase, OGT, Pk-MGMT, SSO2487, Tk-MGMT, TTHA1564

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.63 methylated-DNA-[protein]-cysteine S-methyltransferase

Cloned

Cloned on EC 2.1.1.63 - methylated-DNA-[protein]-cysteine S-methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA is cloned into a pET-11a plasmid and expressed in Escherichia coli
-
expressed at high levels in Escherichia coli fused to an N-terminal polyhistidine tag that allows a single-step isolation and purification by metal-affinity chromatography
expressed in CHO-9 cells
-
expressed in Escherichia coli
expressed in Escherichia coli ABLE-C cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21-Codon Plus (DE3)-RIL cells
-
expressed in Escherichia coli Rosetta(DE3) cells
-
expressed in Escherichia coli XL-1 Blue cells
expressed in Escherichia coliKT233 and in HeLa cells
-
expressed in HEK-293T cells as enhanced green fluorescent protein-tagged enzyme
-
expressed in U-87MG cells
-
expression in CHO cells
-
expression in Escherichia coli
expression in Escherichia coli strain AB1157 and isogenic strain GWR111
expression in Escherichia coli TRG8
-
expression in Escherichia coli, transgenic overexpression in mouse skin, expression in CHO cells
-
expression of N-hAGT and C-hAGT in Escherichia coli XL1-Blue cells. N-hAGT, C-hAGT and full length AGT expressed from the pQE-30 vector in Escherichia coli GWR-109
-
ligated into the pHybLex/Zeo plasmid and electroporated into Escherichia coli XL1-Blue, yielding a library of at least 320000 independent clones, genes of selected AGT mutants subcloned into the pGEX-2T plasmid, allowing for the expression of the AGT mutants as GST fusion proteins
-
mutants subcloned into pGEX-2T for expression as a GST–AGT fusion, or into pET15b for expression as a 6 x His–AGT fusion protein
overexpressed in Escherichia coli strain UC978
-
overexpression in CHO-derived Tet-on-inducible cells, knockdown of MGMT expression with small interfering RNA in HONE-1 cells
-
recombinant AGT and mutants expressed in Escherichia coli, pQE expression vectors for wild-type and mutant AGT production and plasmid pREP4 cotransformed into TRG8 cells to suppress basal AGT protein expression
-
recombinant variants are expressed in HMS174 pLyS cells
-
the gene is placed under the control of the lac promoter and overexpressed in the methyltransferase-deficient Escherichia coli strain KT233
-
transformed into Escherichia coli BL21(DE3)
-