2.1.1.63: methylated-DNA-[protein]-cysteine S-methyltransferase
This is an abbreviated version!
For detailed information about methylated-DNA-[protein]-cysteine S-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.63
-
2.1.1.63
-
glioblastoma
-
temozolomide
-
glioma
-
o6-benzylguanine
-
adduct
-
resect
-
hypermethylation
-
methylation-specific
-
radiotherapy
-
unmethylated
-
mismatch
-
nitrosoureas
-
isocitrate
-
cpg
-
n-methyl-n'-nitro-n-nitrosoguanidine
-
multiforme
-
chloroethylnitrosoureas
-
progression-free
-
1,3-bis2-chloroethyl-1-nitrosourea
-
chloroethylating
-
n-methyl-n-nitrosourea
-
astrocytomas
-
carmustine
-
o6-meg
-
interstrand
-
glycosylase
-
dna-repair
-
anaplastic
-
karnofsky
-
dacarbazine
-
repair-deficient
-
tmz-induced
-
methylnitrosourea
-
phosphotriester
-
n-nitroso
-
myelosuppression
-
chemoradiotherapy
-
oligodendrogliomas
-
tmz-resistant
-
medicine
-
procarbazine
-
p14arf
-
drug development
-
diagnostics
-
lomustine
-
miscoding
-
idh-mutant
-
analysis
-
idh-wildtype
-
dna-alkylating
-
repair-proficient
-
neuro-oncology
-
premutagenic
-
codeletion
-
molecular biology
- 2.1.1.63
- glioblastoma
- temozolomide
- glioma
- o6-benzylguanine
- adduct
-
resect
-
hypermethylation
-
methylation-specific
-
radiotherapy
-
unmethylated
- mismatch
-
nitrosoureas
- isocitrate
- cpg
- n-methyl-n'-nitro-n-nitrosoguanidine
- multiforme
-
chloroethylnitrosoureas
-
progression-free
-
1,3-bis2-chloroethyl-1-nitrosourea
-
chloroethylating
- n-methyl-n-nitrosourea
- astrocytomas
- carmustine
-
o6-meg
-
interstrand
- glycosylase
-
dna-repair
-
anaplastic
-
karnofsky
- dacarbazine
-
repair-deficient
-
tmz-induced
-
methylnitrosourea
-
phosphotriester
-
n-nitroso
-
myelosuppression
-
chemoradiotherapy
- oligodendrogliomas
-
tmz-resistant
- medicine
- procarbazine
-
p14arf
- drug development
- diagnostics
-
lomustine
-
miscoding
-
idh-mutant
- analysis
-
idh-wildtype
-
dna-alkylating
-
repair-proficient
-
neuro-oncology
-
premutagenic
-
codeletion
- molecular biology
Reaction
Synonyms
Ada protein, Ada-C, AGAT, AGT, AGTendoV, alkylated DNA-protein alkyltransferase, alkylguanine transferase, ATASE, C-hAGT, carboxyl terminal domain of the inducible Escherichia coli ada alkyltransferase, hAGT, methylated-DNA-[protein]-cysteine S-methyltransferase, methylguanine DNA methyltransferase, MGMT, Mgt1, MJ1529, N-hAGT, O6-alkylguanine DNA alkyltransferase, O6-alkylguanine DNA-alkyltransferase, O6-alkylguanine-DNA alkyl-transferase, O6-alkylguanine-DNA alkyltransferase, O6-methylguanine DNA methyltransferase, O6-methylguanine methyltransferase, O6-methylguanine-DNA methyltransferase, O6-methylguanine-DNA-methyltransferase, O6-MGMT, O6meG-DNA alkyltransferase, OGT, Pk-MGMT, SSO2487, Tk-MGMT, TTHA1564
ECTree
2.1.1.63 methylated-DNA-[protein]-cysteine S-methyltransferaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 2.1.1.63 - methylated-DNA-[protein]-cysteine S-methyltransferase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
DNA (containing 6-O-benzylguanine) + protein L-cysteine
DNA (without 6-O-benzylguanine) + protein S-benzyl-L-cysteine
-
-
-
-
?
DNA (containing 6-O-carboxymethylguanine) + protein L-cysteine
DNA (without 6-O-carboxymethylguanine) + protein S-carboxymethyl-L-cysteine
-
-
-
-
?
DNA (containing 6-O-methylguanine) + protein L-cysteine
DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
DNA (lacking 6-O-methylguanine) + protein S-methyl-L-cysteine
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
DNA (containing 6-O-methylguanine) + [protein]-L-cysteine
DNA (without 6-O-methylguanine) + [protein]-S-methyl-L-cysteine
DNA (containing O6-chloroethylguanine) + protein L-cysteine
DNA (without O6-chloroethylguanine) + protein S-chloroethyl-L-cysteine
-
-
-
-
?
DNA (containing O6-[4-oxo-4-(3-pyridyl)butyl]guanine) + protein L-cysteine
DNA (without O6-[4-oxo-4-(3-pyridyl)butyl]guanine) + protein S-4-oxo-4-(3-pyridyl)butyl-L-cysteine
-
-
-
-
?
DNA containing 4-O-methylthymine + [protein-L-cysteine
DNA lacking 4-O-methylthymine + [protein]-S-methyl-L-cysteine
DNA containing 4-O-methylthymine + [protein]-L-cysteine
DNA lacking 4-O-methylguanine + [protein]-S-methyl-L-cysteine
-
-
-
-
?
DNA containing 6-O-benzylguanine + [protein]-L-cysteine
DNA lacking 6-O-benzylguanine + [protein]-S-methyl-L-cysteine
-
AGT binds and scans DNA rapidly, flips O6-alkylG residues, transfers the alkyl group in a chemical step that is not rate-limiting in the case of 6-O-benzylguanine and releases the dealkylated DNA
-
-
?
DNA containing 6-O-ethylguanine + [protein]-L-cysteine
DNA lacking 6-O-ethylguanine +[protein]-S-ethyl-L-cysteine
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
[N-[2-(2-[2-[(3-[[(2-amino-9H-purin-6-yl)oxy]methyl]phenyl)methoxy]ethoxy]ethoxy)ethyl]-5-(3,5-dimethyl-1H-pyrrol-2-yl-kappaN)-5-(3,5-dimethyl-2H-pyrrol-2-ylidene-kappaN)pentanamidato](difluoro)boron + [protein]-L-cysteine
[protein]-S-methyl-L-cysteine + ?
-
-
-
-
?
DNA (containing 6-O-methylguanine) + protein L-cysteine
DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine
-
-
-
-
?
DNA (containing 6-O-methylguanine) + protein L-cysteine
DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine
-
-
-
?
DNA (containing 6-O-methylguanine) + protein L-cysteine
DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine
-
-
-
-
?
DNA (containing 6-O-methylguanine) + protein L-cysteine
DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine
-
-
-
?
DNA (containing 6-O-methylguanine) + protein L-cysteine
DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine
-
-
-
-
?
DNA (containing 6-O-methylguanine) + protein L-cysteine
DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine
-
-
-
?
DNA (containing 6-O-methylguanine) + protein L-cysteine
DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine
-
-
-
-
?
DNA (containing 6-O-methylguanine) + protein L-cysteine
DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine
-
-
-
-
?
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
DNA (lacking 6-O-methylguanine) + protein S-methyl-L-cysteine
-
-
-
?
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
DNA (lacking 6-O-methylguanine) + protein S-methyl-L-cysteine
-
-
-
?
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
-
-
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein
-
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
-
-
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein
-
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
-
-
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein
-
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
-
-
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein
-
-
?
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
-
-
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein
-
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
-
-
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein
-
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
-
-
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein
-
-
ir
DNA (containing 6-O-methylguanine) + [protein]-L-cysteine
DNA (without 6-O-methylguanine) + [protein]-S-methyl-L-cysteine
-
-
-
-
?
DNA (containing 6-O-methylguanine) + [protein]-L-cysteine
DNA (without 6-O-methylguanine) + [protein]-S-methyl-L-cysteine
-
-
-
?
DNA (containing 6-O-methylguanine) + [protein]-L-cysteine
DNA (without 6-O-methylguanine) + [protein]-S-methyl-L-cysteine
-
-
-
-
?
DNA (containing 6-O-methylguanine) + [protein]-L-cysteine
DNA (without 6-O-methylguanine) + [protein]-S-methyl-L-cysteine
-
-
-
?
DNA (containing 6-O-methylguanine) + [protein]-L-cysteine
DNA (without 6-O-methylguanine) + [protein]-S-methyl-L-cysteine
-
-
-
?
DNA containing 4-O-methylthymine + [protein-L-cysteine
DNA lacking 4-O-methylthymine + [protein]-S-methyl-L-cysteine
-
-
-
-
?
DNA containing 4-O-methylthymine + [protein-L-cysteine
DNA lacking 4-O-methylthymine + [protein]-S-methyl-L-cysteine
-
-
-
-
?
DNA containing 4-O-methylthymine + [protein-L-cysteine
DNA lacking 4-O-methylthymine + [protein]-S-methyl-L-cysteine
-
enzyme repairs O6-methylguanine lesions in DNA via alkyl transfer action
-
-
?
DNA containing 4-O-methylthymine + [protein-L-cysteine
DNA lacking 4-O-methylthymine + [protein]-S-methyl-L-cysteine
-
-
-
-
?
DNA containing 4-O-methylthymine + [protein-L-cysteine
DNA lacking 4-O-methylthymine + [protein]-S-methyl-L-cysteine
-
very poor substrate
-
-
?
DNA containing 4-O-methylthymine + [protein-L-cysteine
DNA lacking 4-O-methylthymine + [protein]-S-methyl-L-cysteine
-
DNA-repair enzyme
-
-
?
DNA containing 6-O-ethylguanine + [protein]-L-cysteine
DNA lacking 6-O-ethylguanine +[protein]-S-ethyl-L-cysteine
-
CGC(e6G)AGCTCGCG
-
?
DNA containing 6-O-ethylguanine + [protein]-L-cysteine
DNA lacking 6-O-ethylguanine +[protein]-S-ethyl-L-cysteine
-
CGC(e6G)AGCTCGCG
-
?
DNA containing 6-O-ethylguanine + [protein]-L-cysteine
DNA lacking 6-O-ethylguanine +[protein]-S-ethyl-L-cysteine
-
CGC(e6G)AGCTCGCG
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
DNA-repair protein that protects cells from killing and mutagenesis by alkylating agents
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
DNA-repair protein that protects cells from killing and mutagenesis by alkylating agents
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
-
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
synthetic DNA polymer poly(dC,dG,m6dG)
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
CGC(m6G)CG and CGC(m6G)AGCTCGCG
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
transferase activity methylates itself on removal of the methyl group from the 6-O position of guanine
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
the enzyme functions in DNA repair by direct dealkylation of mutagenic 6-O-alkylguanine. The protein methylated at Cys69 becomes a transcriptional activator of the genes in the ada regulon, including its own
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
6-O-methylguanine is an important adduct formed by methylating agents, that, if not repaired, can lead to mutations and death. Its repair is carried out by 6-O-methylguanine DNA-methyltransferase. Exposure of E. coli cells to sublethal concentrations of methylating agent triggers the expression of the gene
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
-
485492, 485496, 485498, 485501, 485502, 485503, 485506, 672820, 675913, 676204, 703079, 703105, 703393, 703539, 703974, 704064, 704813, 705274, 705497, 705661, 705829, 705897, 718566, 719586
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
oligonucleotides containing an 6-O-(2-fluorobenzyl)guanine, 6-O-(3-fluorobenzyl)guanine, 6-O-benzylhypoxanthine or 6-O-methylguanine are all good substrates
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
enzyme reacts with the alkylated base in a synthetic DNA substrate poly(dC, dG, m6dG)
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
the binding to DNA is the rate determining step in the repair process. Approximately eight base pairs of the DNA substrate are covered by the human enzyme. Binding affinity to methylated DNA is two times higher than that to unmodified DNA. The interaction with DNA induces a conformational change in the enzyme
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
the alkyl group is transferred without a cofactor to Cys145 residue of the enzyme and thereby inactivates the protein
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
the methyltransferase protein itself accepts methyl groups from methylated DNA, one enzyme molecule accepts one methyl group
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
the enzyme repairs DNA by transferring alkyl (R)-adducts from 6-O-alkylguanine in DNA to its own cysteine residue at codon 145
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
the enzyme is a critical defense against alkylation-induced mutagenesis and carcinogenesis
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
the enzyme repairs mutagenic and carcinogenic 6-O-alkylguanine in DNA
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
DNA hypermethylation and silencing of MGMT are frequent and rather early events in esophageal squamous cell carcinogenesis. Hypermethylation and inactivation of MGMT may be prevented or reversed by dietary polyphenols, (-)-epigallocatechin-3-gallate and genistein, for the prevention of carcinogenesis
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
key enzyme in DNA repair network. Hypermethylation of the CpG island located in the promoter region of MGMT is primarily responsible for the loss of enzyme function in many tumor types. The methylation mediated silencing of MGMT has two consequences for cancer. First, tumors with MGMT methylation have a new mutator phenotype characterized by the generation of transition point mutations in genes involved in cancer etiology, such as the tumor suppressor p53 and the oncogene K-ras. Second, MGMT hypermethylation demonstrates the possibility of pharmacoepigenomics: methylated tumors are more sensitive to the killing effects of alkylating drugs used in chemotherapy
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
the protein protects the integrity of the genome, and it also contributes to the resistance of tumors to DNA-alkylating chemotherapeutic agents
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
DNA-binding mechanism
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
AGT binds and scans DNA rapidly, flips O6-alkylG residues, transfers the alkyl group in a chemical step that is rate-limiting in the case of 6-O-methylguanine and releases the dealkylated DNA
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
C-terminal domain is totally inactive, the N-terminal domain has a very weak but definite activity that is totally dependent on the refolding being carried out in the presence of zinc ions
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
prefered substrate
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
-
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
-
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
enzyme reacts with the alkylated base in a synthetic DNA substrate poly(dC, dG, m6dG)
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
the enzyme repairs mutagenic and carcinogenic 6-O-alkylguanine in DNA
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
-
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
-
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
-
-
-
?
DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
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CGC(m6G)CG and CGC(m6G)AGCTCGCG
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DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
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the DNA repair enzyme can specifically remove methyl groups from 6-O-methylguanine, which is a major mutagenic and carcinogenic DNA lesion leading to the G-A transition
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DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
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DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
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DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
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the enzyme repairs alkylated DNA by suicidal alkyl transfer from guanine 6-O to its own cysteine residue
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DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
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6-O-methylguanine oligonucleotide
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DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
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6-O-methylguanine oligonucleotide
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DNA containing 6-O-methylguanine + [protein]-L-cysteine
DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
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6-O-methylguanine oligonucleotide
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SNAP-Vista Green + [protein]-L-cysteine
guanine + [protein]-S-Vista Green-L-cysteine
fluorescent 6-O-benzylguanine derivative
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SNAP-Vista Green + [protein]-L-cysteine
guanine + [protein]-S-Vista Green-L-cysteine
fluorescent 6-O-benzylguanine derivative
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additional information
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the 28-amino acid carboxy-terminal tail of the enzyme is not required for activity and modulates the rate of 6-O-methylguanine DNA methyltransferase repair at reduced temperatures and plays a role in substrate specificity
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additional information
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binding of AGT to single-stranded DNAs ranging in length from 5 to 78 nucleotides, binding is moderately cooperative, resulting in an all-or-nothing association pattern on short templates binding density
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additional information
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binding of AGT to single-stranded DNAs ranging in length from 5 to 78 nucleotides, binding is moderately cooperative, resulting in an all-or-nothing association pattern on short templates binding density
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additional information
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in addition to repairing methyl groups, longer alkyl groups including ethyl-, n-propyl-, n-butyl-, 2-chloroethyl-, 2-hydroxyethyl-, iso-propyl and iso-butyl can be repaired
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additional information
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low reactivity of the non-natural substrate O6-propargylguanine with AGT, most likely arises from the poor binding of the substrate to the active site of AGT
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additional information
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human AGT acts poorly on DNA containing 4-O-methylthymine
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additional information
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in the catalytic demethylation of DNA containing 6-O-methylguanine, roles of six amino acids, i.e., Cys145, His146, Glu172, Tyr114, Lys165, and Ser159 are involved. At the first step, Cys145 in the Cys145-water-His146-Glu172 tetrad is converted to cysteine thiolate anion while at the second step, abstraction of the Tyr114 proton by the N3 site of DNA containing 6-O-methylguanine occurs in a barrierless manner. In the third step, abstraction of Lys165 proton by deprotonated Tyr114 and transfer of the methyl group of DNA containing 6-O-methylguanine to the thiolate group of Cys145 anion occur simultaneously.
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additional information
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TTHA1564 can bind to DNA containing 6-O-methylguanine with higher affinity (9fold) than normal (unmethylated) DNA, but TTHA1564 alone does not possess methyltransferase activity
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additional information
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TTHA1564 can interact with nucleotide excision repair proteins and RNA polymerase
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additional information
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Thermus thermophilus HB8 / ATCC 27634 / DSM 579
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TTHA1564 can bind to DNA containing 6-O-methylguanine with higher affinity (9fold) than normal (unmethylated) DNA, but TTHA1564 alone does not possess methyltransferase activity
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additional information
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Thermus thermophilus HB8 / ATCC 27634 / DSM 579
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TTHA1564 can interact with nucleotide excision repair proteins and RNA polymerase
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