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2.1.1.228: tRNA (guanine37-N1)-methyltransferase

This is an abbreviated version!
For detailed information about tRNA (guanine37-N1)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.228

Reaction

S-adenosyl-L-methionine
+
guanine37 in tRNA
=
S-adenosyl-L-homocysteine
 +
N1-methylguanine37 in tRNA

Synonyms

At3g56120, aTrm5a methyltransferase, aTrm5a/Taw22-like enzyme, AtTrm5a, bacterial tRNA (guanine37-N1)-methyltransferase, bacterial tRNA-(N1G37) methyltransferase, EC 2.1.1.31, EcTrmD, HiTrmD, HsTrm5, m1G37 tRNA methyltransferase, m1G37-forming tRNA methyltransferase, Mj-Trm5, MJ0883, MjTrm5, MjTrm5b, mono-functional methyltransferase, More, MtbTrmD, NEQ228, PA14_15990, PAB2272, PAB_RS03940, PaTrm5a, PaTrm5b, PaTrmD, SaTrmD, Ta0997, TAW22, transfer RNA (m1G37) methyltransferase, Trm1, TRM5, Trm5a, Trm5a/Taw22-like enzyme, trm5b, Trm5p, TrmD, TRMT5, tRNA (guanine(37)-N1)-methyltransferase 1, tRNA (guanine37-N1)-methyltransferase, tRNA (guanosine-1) methyltransferase, tRNA (m1G37) methyltransferase, tRNA (m1G37)-methyltransferase, tRNA m1G37 methyltransferase, tRNA methyltransferase, tRNA methyltransferase 5, tRNA methyltransferase D, tRNA(m(1)G37)methyltransferase, tRNA(m1G37) methyltransferase, tRNA(m1G37) MTase, tRNA(m1G37)methyltransferase, tRNA-(N1G37) methyltransferase, tRNAPhe:imG2 methyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.228 tRNA (guanine37-N1)-methyltransferase

Cloned

Cloned on EC 2.1.1.228 - tRNA (guanine37-N1)-methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli by using a His-tagged T7 expression vector
-
expression in Escherichia coli
expression in Escherichia coli as a fusion protein with maltose-binding protein
gene At3g56120, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21, recombinant expression in and full functional complementation of Saccgaromyces cerevisiae DELTAtrm5 mutant with the AtTRM5a gene. Yeast GBY15 mutant is defective for m1G37 nucleoside modification, and the mutant strain grows slow compared to the congenic strain GBY16
gene PA14_15990, sequence comparisons, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Rosetta T1R
gene TRM5, recombinant expression of C-terminally His-tagged enzyme, lacking the N-terminal peptide V2LWILWRP9, in Escherichia coli
gene trm5, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene trm5a, recombinant expression of His6-tagged wild-type and mutant enzymes
gene trm5a, sequence comparisons, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene trmD, recombinant expression of His-tagged enzyme from AVA0421 plasmid with an N-His-3C protease site in Escherichia coli strain BL21(DE3)
-
gene trmD, recombinant expression of His-tagged full-length enzyme in Escherichia coli strain BL21(DE3)
gene trmD, recombinant expression of His-tagged isolated N-terminal domain in Escherichia coli strain BL21(DE3)
gene trmD, recombinant expression of N-terminally His-tagged wild-type and S88L mutant enzymes in Escherichia coli strain SG13009
gene trmD, sequence comparisons
gene TRMT5, genetic structure and genotyping
-
recombinant expression of His-tagged enzyme
recombinant expression of His-tagged wild-type and mutant enzymes
recombinant overexpression of His6-tagged full-length PaTrm5b and PaTrm5b73-330 mutant in Escherichia coli strain BL21(DE3)
recombinant TrmD from Haemophilus influenzae with both N- and C-terminal tags is overexpressed in Escherichia coli
-