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Literature summary for 2.1.1.228 extracted from

  • Elkins, P.A.; Watts, J.M.; Zalacain, M.; van Thiel, A.; Vitazka, P.R.; Redlak, M.; Andraos-Selim, C.; Rastinejad, F.; Holmes, W.M.
    Insights into catalysis by a knotted TrmD tRNA methyltransferase (2003), J. Mol. Biol., 333, 931-949.
    View publication on PubMed
Search for more literature for 2.1.1.228

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of TrmD complexed with S-adenosyl homocysteine, determined at 2.5 A resolution Escherichia coli

Protein Variants

Protein Variants Comment Organism
A202S Km/Vmax for tRNA is 2fold higher than wild-type value Escherichia coli
A25S Km/Vmax for tRNA is 2.9fold higher than wild-type value Escherichia coli
A70S Km/Vmax for tRNA is 4fold higher than wild-type value Escherichia coli
C112A Km/Vmax for tRNA is 7.6fold higher than wild-type value Escherichia coli
D119A inactive mutant enzyme Escherichia coli
D128A inactive mutant enzyme Escherichia coli
D135A inactive mutant enzyme Escherichia coli
D169A inactive mutant enzyme Escherichia coli
D169E Km/Vmax for tRNA is 1.4fold higher than wild-type value Escherichia coli
D50A Km/Vmax for tRNA is 4fold higher than wild-type value Escherichia coli
E116A Km/Vmax for tRNA is 2fold higher than wild-type value Escherichia coli
E130A Km/Vmax for tRNA is 2fold higher than wild-type value Escherichia coli
E142A Km/Vmax for tRNA is 3.1fold higher than wild-type value Escherichia coli
G113A Km/Vmax for tRNA is 5.3fold higher than wild-type value Escherichia coli
G117A inactive mutant enzyme Escherichia coli
G134A Km/Vmax for tRNA is 6.8fold higher than wild-type value Escherichia coli
G140A Km/Vmax for tRNA is 8.5fold higher than wild-type value Escherichia coli
G141A Km/Vmax for tRNA is 1.5fold lower than wild-type value Escherichia coli
G189A Km/Vmax for tRNA is 8fold higher than wild-type value Escherichia coli
G55A Km/Vmax for tRNA is 4.8fold higher than wild-type value Escherichia coli
G59A inactive mutant enzyme Escherichia coli
G91A inactive mutant enzyme Escherichia coli
H180A Km/Vmax for tRNA is 5 fold higher than wild-type value Escherichia coli
I204A inactive mutant enzyme Escherichia coli
L138A Km/Vmax for tRNA is 1,7fold higher than wild-type value Escherichia coli
L196A inactive mutant enzyme Escherichia coli
L197A inactive mutant enzyme Escherichia coli
M60A Km/Vmax for tRNA is 2.7fold higher than wild-type value Escherichia coli
P184A inactive mutant enzyme Escherichia coli
P193A inactive mutant enzyme Escherichia coli
P53A Km/Vmax for tRNA is 2fold higher than wild-type value Escherichia coli
R114A inactive mutant enzyme Escherichia coli
R121A inactive mutant enzyme Escherichia coli
R154A inactive mutant enzyme Escherichia coli
R208A inactive mutant enzyme Escherichia coli
R215A Km/Vmax for tRNA is 5fold higher than wild-type value Escherichia coli
R219A Km/Vmax for tRNA is 4fold higher than wild-type value Escherichia coli
S132A Km/Vmax for tRNA is 1.5fold higher than wild-type value Escherichia coli
V192A inactive mutant enzyme Escherichia coli
W131A Km/Vmax for tRNA is 1.3fold higher than wild-type value Escherichia coli
W207A inactive mutant enzyme Escherichia coli
W207F Km/Vmax for tRNA is 4fold higher than wild-type value Escherichia coli
W207H Km/Vmax for tRNA is 5.6fold higher than wild-type value Escherichia coli
Y136A Km/Vmax for tRNA is 7.3fold higher than wild-type value Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 gel filtration Escherichia coli
64000
-
 dynamic light-scattering Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A873
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Subunits

Subunits Comment Organism
dimer each monomer consists of a C-terminal domain connected by a flexible linker to an N-terminal AdoMet-binding domain Escherichia coli

Synonyms

Synonyms Comment Organism
TrmD
-
 Escherichia coli
tRNA (guanosine-1) methyltransferase
-
 Escherichia coli