Any feedback?
Literature summary for 2.1.1.228 extracted from
- Conservation of structure and mechanism by Trm5 enzymes (2013), RNA, 19, 1192-1199.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene TRM5, recombinant expression of C-terminally His-tagged enzyme, lacking the N-terminal peptide V2LWILWRP9, in Escherichia coli | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D275A | site-directed mutagenesis, the mutation leads to significantly reduced activity | Homo sapiens |
| E288A | site-directed mutagenesis, the mutation at the general base position leads to highly reduced activity | Homo sapiens |
| E394K | site-directed mutagenesis, the mutation facilitates enzyme expression in Escherichia coli | Homo sapiens |
| H289A | site-directed mutagenesis, the mutation C-terminally adjacent to the general base does not affect the enzyme activity | Homo sapiens |
| H289R | site-directed mutagenesis, the mutation C-terminally adjacent to the general base does not affect the enzyme activity | Homo sapiens |
| M261L | site-directed mutagenesis, the single M261L substitution that recapitulates the archaeal residue minimizes the 27-kDa protease product upon enzyme expression in Escherichia coli, indicating improved stability | Homo sapiens |
| M261L/T261I | site-directed mutagenesis, the double M261L substitution also shows improved stability | Homo sapiens |
| additional information | structure-guided mutational analysis of HsTrm5 in comparison to the archaeal enzyme from Methanococcus jannaschii, MjTrm5, overview. Validation of the MjTrm5 ternary structure as a useful model for HsTrm5 | Homo sapiens |
| T263I | site-directed mutagenesis, the mutation does not affect the enzyme | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | pre-steady-state and steady-state kinetic analysis of wild-type and mutant enzymes, the rate-determining step is product release from the enzyme, kinetic isotope effect, overview | Homo sapiens | |
| 0.00042 | - |
S-adenosyl-L-methionine | recombinant enzyme, pH 7.3, 37°C | Homo sapiens |  |
| 0.00047 | - |
guanine37 in tRNACys | recombinant enzyme, pH 7.3, 37°C | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + guanine37 in tRNA | Homo sapiens | - |
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q32P41 | from the KIAA 1393 plasmid; gene TRM5 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His-tagged enzyme, lacking the N-terminal peptide V2LWILWRP9, from Escherichia coli by metal ion affinity chromatography and anion exchange chromatography | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| S-adenosyl-L-methionine + guanine37 in tRNA = S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | a highly conserved aspartate residue, D275 in Homo sapiens Trm5, in the flexible loop preceding the proposed general base E288 may be involved in the induced-fit movement of the catalytic process | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + guanine37 in tRNA | - |
Homo sapiens | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
| S-adenosyl-L-methionine + guanine37 in tRNACys | - |
Homo sapiens | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNACys | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TRM5 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.023 | - |
guanine37 in tRNACys | recombinant enzyme, pH 7.3, 37°C | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.1 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9.5 | pH-activity profile | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | - |
Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | structure-guided mutational analysis of HsTrm5 in comparison to the archaeal enzyme from Methanococcus jannaschii, MjTrm5, overview. Validation of the MjTrm5 ternary structure as a useful model for HsTrm5 | Homo sapiens |
| additional information | active-site structure and overall structure analysis, molecular modeling using structure PdB ID 2ZZN, overview | Homo sapiens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 50 | - |
guanine37 in tRNACys | recombinant enzyme, pH 7.3, 37°C | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
