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Literature summary for 2.1.1.228 extracted from
- Isolation and characterization of the human tRNA-(N1G37) methyltransferase (TRM5) and comparison to the Escherichia coli TrmD protein (2004), Biochemistry, 43, 9243-9255.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| glycerol | TRM5 performs best between 25 and 30% glycerol, and is less active at lower concentrations. The enzyme retains 45% of its activity in the presence of 50% glycerol | Homo sapiens |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
- |
Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DTT | TrmD has maximal activity below 1 mM DTT and loses 20% of its activity above that value | Escherichia coli | |
| DTT | TRM5 displays maximal activity above 1 mM DTT and loses 20% of its activity below that value | Homo sapiens | |
| glycerol | the Escherichia coli TRmD performs best in the absence of glycerol, its activity decresaing linearly with increasing glycerol concentrations. No activity at 50% glycerol | Escherichia coli | |
| KCl | the enzyme is most active in absence of KCl | Escherichia coli | |
| KCl | TRM5 enzyme is stimulated 4fold by 100 mM KCl. TRM5 tends to lose all activity in 600 mM KCl | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000457 | - |
guanine37 in yeast tRNAPhe possessing an A36G37 sequence | pH 8.0, 37°C | Homo sapiens | |
| 0.0000546 | - |
guanine37 in Escherichia coli tRNA1Leu | pH 8.0, 37°C | Homo sapiens | |
| 0.000075 | - |
guanine37 in yeast tRNAAsp possessing a C36G37 sequence | pH 8.0, 37°C | Homo sapiens | |
| 0.000244 | - |
guanine37 in yeast tRNAAsp possessing a G36G37 sequence | pH 8.0, 37°C | Homo sapiens | |
| 0.00058 | - |
guanine37 in yeast tRNAAsp possessing an U36G37 sequence | pH 8.0, 37°C | Homo sapiens | |
| 0.00059 | - |
guanine37 in yeast tRNAAsp possessing an A36G37 sequence | pH 8.0, 37°C | Homo sapiens | |
| 0.00123 | - |
inosine37 in yeast tRNAAsp possessing a G36I37 sequence | below 0.00123 pH 8.0, 37°C | Homo sapiens | |
| 0.00546 | - |
guanine37 in human mitochondrial tRNAPro | pH 8.0, 37°C | Homo sapiens | |
| 0.0057 | - |
guanine37 in Escherichia coli tRNA1Leu | pH 8.0, 37°C | Escherichia coli | |
| 0.0086 | - |
guanine37 in human mitochondrial tRNAPro | pH 8.0, 37°C | Escherichia coli | |
| 0.018 | - |
guanine37 in human mitochondrial tRNAPro possessing an A36G37 sequence | pH 8.0, 37°C | Homo sapiens | |
| 0.019 | - |
guanine37 in yeast tRNAAsp possessing a G36G37 sequence | pH 8.0, 37°C | Escherichia coli | |
| 0.046 | - |
guanine37 in yeast tRNAAsp possessing an A36G37 sequence | pH 8.0, 37°C | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | TRM5 enzyme is stimulated 4fold by 100 mM KCl. TRM5 tends to lose all activity in 600 mM KCl | Homo sapiens | |
| Mg2+ | totally inactive without Mg2+ | Escherichia coli | |
| additional information | no absolute requirement for Mg2+ | Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30586 | - |
2 * 30586, calculated from sequence | Escherichia coli |
| 60500 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + guanine37 in tRNA | Escherichia coli | - |
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
| S-adenosyl-L-methionine + guanine37 in tRNA | Homo sapiens | - |
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0A873 | - |
- |
| Homo sapiens | Q32P41 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | guanosine37-methylation by TRM5 occurs regardless of the nature of the nucleotide at position 36. TRM5 also methylates inosine at position 37. The TRM5 enzyme is sensitive to subtle changes in the tRNA-protein tertiary interaction leading to loss of activity. The enzyme does not methylate adenosine37, cytosine37 or uridine37 in tRNA. The TRM5 enzyme is sensitive to subtle changes in the tRNA-protein tertiary interaction leading to loss of activity | Homo sapiens | ? | - |
? | |
| additional information | TrmD recognizes the G36pG37 motif preferentially and does not methylate inosine. The TrmD enzyme is tolerant of alterations in tRNA-protein tertiary interactions as long as the core tRNA structure and the G36pG37 are present | Escherichia coli | ? | - |
? | |
| S-adenosyl-L-methionine + guanine37 in Escherichia coli tRNA1Leu | - |
Escherichia coli | S-adenosyl-L-homocysteine + N1-methylguanine37 in Escherichia coli tRNA1Leu | - |
? | |
| S-adenosyl-L-methionine + guanine37 in Escherichia coli tRNA1Leu | - |
Homo sapiens | S-adenosyl-L-homocysteine + N1-methylguanine37 in Escherichia coli tRNA1Leu | - |
? | |
| S-adenosyl-L-methionine + guanine37 in human mitochondrial tRNAPro | - |
Escherichia coli | S-adenosyl-L-homocysteine + N1-methylguanine37 in human mitochondrial tRNAPro | - |
? | |
| S-adenosyl-L-methionine + guanine37 in human mitochondrial tRNAPro | - |
Homo sapiens | S-adenosyl-L-homocysteine + N1-methylguanine37 in human mitochondrial tRNAPro | - |
? | |
| S-adenosyl-L-methionine + guanine37 in human mitochondrial tRNAPro possessing an A36G37 sequence | - |
Homo sapiens | S-adenosyl-L-homocysteine + N1-methylguanine37 in human mitochondrial tRNAPro possessing an A36G37 sequence | - |
? | |
| S-adenosyl-L-methionine + guanine37 in tRNA | - |
Escherichia coli | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
| S-adenosyl-L-methionine + guanine37 in tRNA | - |
Homo sapiens | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
| S-adenosyl-L-methionine + guanine37 in tRNA | methylates the N1 position of guanosine 37 (G37) in selected tRNA transcripts | Escherichia coli | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
| S-adenosyl-L-methionine + guanine37 in tRNA | methylates the N1 position of guanosine 37 (G37) in selected tRNA transcripts | Homo sapiens | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
| S-adenosyl-L-methionine + guanine37 in yeast tRNA(Asp) possessing a G36G37 sequence | - |
Homo sapiens | S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNA(Asp) possessing a G36G37 sequence | - |
? | |
| S-adenosyl-L-methionine + guanine37 in yeast tRNAAsp possessing a C36G37 sequence | - |
Homo sapiens | S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNAAsp possessing a C36G37 sequence | - |
? | |
| S-adenosyl-L-methionine + guanine37 in yeast tRNAAsp possessing a G36G37 sequence | - |
Escherichia coli | S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNAAsp possessing a G36G37 sequence | - |
? | |
| S-adenosyl-L-methionine + guanine37 in yeast tRNAAsp possessing a G36G37 sequence | - |
Homo sapiens | S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNAAsp possessing a G36G37 sequence | - |
? | |
| S-adenosyl-L-methionine + guanine37 in yeast tRNAAsp possessing an A36G37 sequence | - |
Homo sapiens | S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNA(Asp) possessing an A36G37 sequence | - |
? | |
| S-adenosyl-L-methionine + guanine37 in yeast tRNAAsp possessing an A36G37 sequence | - |
Escherichia coli | S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNAAsp possessing an A36G37 sequence | - |
? | |
| S-adenosyl-L-methionine + guanine37 in yeast tRNAAsp possessing an U36G37 sequence | - |
Homo sapiens | S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNAAsp possessing an U36G37 sequence | - |
? | |
| S-adenosyl-L-methionine + guanine37 in yeast tRNAPhe possessing an A36G37 sequence | - |
Homo sapiens | S-adenosyl-L-homocysteine + N1-methylguanine37 in yeast tRNAPhe possessing an A36G37 sequence | - |
? | |
| S-adenosyl-L-methionine + inosine37 in yeast tRNAAsp possessing a G36I37 sequence | - |
Homo sapiens | S-adenosyl-L-homocysteine + N1-methylinosine37 in yeast tRNAAsp possessing a G36I37 sequence | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 30586, calculated from sequence | Escherichia coli |
| monomer | 1 * 60500, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TRM5 | - |
Homo sapiens |
| TrmD | - |
Escherichia coli |
| tRNA-(N1G37) methyltransferase | - |
Escherichia coli |
| tRNA-(N1G37) methyltransferase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Escherichia coli |
| 7.4 | - |
- |
Homo sapiens |
| 8 | - |
assay at | Escherichia coli |
| 8 | - |
assay at | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | 8.4 | fairly active between pH 6.4 and pH 8.4 | Escherichia coli |
| 6.4 | 8.4 | fairly active between pH 6.4 and pH 8.4 | Homo sapiens |
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