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1.4.1.9: leucine dehydrogenase

This is an abbreviated version!
For detailed information about leucine dehydrogenase, go to the full flat file.

Word Map on EC 1.4.1.9

Reaction

L-leucine
+
H2O
+
NAD+
=
4-methyl-2-oxopentanoate
+
NH3
+
NADH
+
H+

Synonyms

BCD, dehydrogenase, leucine, L-leucine dehydrogenase, L-leucine:NAD+ oxidoreductase, deaminating, LeuDH

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.9 leucine dehydrogenase

Molecular Weight

Molecular Weight on EC 1.4.1.9 - leucine dehydrogenase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
245000
-
equilibrium sedimentation
280000
300000
-
gel filtration
310000
-
gel filtration, equilibrium sedimenation
313000
-
non-denaturing PAGE
320000
-
gel filtration
325000
-
gel filtration
330000
-
gel filtration
340000
350000
-
gel filtration
360000
39000
40000
-
2 * 40000, SDS-PAGE
41000
-
6 * 41000, SDS-PAGE
42000
-
4 * 42000, SDS-PAGE
43000
44000
-
8 * 44000, SDS-PAGE
46903
-
x * 46903, calculation from nucleotide sequence
47000
-
6 * 47000, SDS-PAGE
49000
-
6 * 49000, SDS-PAGE
55000
-
6 * 55000, at 2.5 M NaCl, dissociates into trimers and dimers in a lower concentration of salts, cetyltrimethylammonium-bromide-PAGE
56000
-
6 * 56000, SDS-PAGE
82000
-
mutant enzyme LeuDEL4, gel filtration
additional information
-
MW of the chimeric enzyme consisting of an amino-terminal domain of phenylalanine dehydrogenase and a carboxy-terminal domain of leucine dehydrogenase is 72000 Da, determined by gel filtration