1.4.1.9: leucine dehydrogenase
This is an abbreviated version!
For detailed information about leucine dehydrogenase, go to the full flat file.
Word Map on EC 1.4.1.9
-
1.4.1.9
-
sphaericus
-
l-valine
-
synthesis
-
l-isoleucine
-
l-2-aminobutyric
-
l-tert-leucine
-
intermedius
-
thermoactinomyces
-
l-threonine
-
analysis
-
space-time
-
3.5.1.5
-
trimethylpyruvate
-
alpha-ketoisocaproate
-
lysinibacillus
-
drug development
-
biotechnology
- 1.4.1.9
- sphaericus
- l-valine
- synthesis
- l-isoleucine
-
l-2-aminobutyric
- l-tert-leucine
- intermedius
-
thermoactinomyces
- l-threonine
- analysis
-
space-time
-
3.5.1.5
- trimethylpyruvate
- alpha-ketoisocaproate
-
lysinibacillus
- drug development
- biotechnology
Reaction
Synonyms
BCD, dehydrogenase, leucine, L-leucine dehydrogenase, L-leucine:NAD+ oxidoreductase, deaminating, LeuDH
ECTree
1.4.1.9 leucine dehydrogenaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 1.4.1.9 - leucine dehydrogenase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-keto-beta-methylvalerate + NH3 + NADH
? + H2O + NAD+
-
as active as 2-ketoisocaproate, wild-type enzyme
-
-
?
2-keto-gamma-methylthiobutanoate + NH3 + NADH
L-Met + H2O + NAD+
-
15% of the activity with 2-ketoisocaproate, wild-type enzyme
-
-
?
2-ketocaproate + NH3 + NADH
L-2-aminohexanoate + H2O + NAD+
-
69% of the activity with 2-ketoisocaproate
-
-
?
2-ketoisovalerate + NH3 + NADH
L-Val + H2O + NAD+
-
150% of the activity with 2-ketoisocaproate
-
-
?
2-oxo-3,3-dimethylbutanoate + NH3 + NADH
L-2-amino-3,3-dimethylbutanoate + NAD+ + H2O
-
-
-
-
?
2-oxo-4-methylselenobutyrate + NH3 + NADH
L-selenomethionine + H2O + NAD+
-
-
-
?
2-oxobutanoate + NH3 + NADH
L-2-aminobutanoate + H2O + NAD+
-
11% activity 2-oxobutanoate compared to 4-methyl-2-oxopentanoate
-
-
r
2-oxopentanoate + NH3 + NADH
L-norvaline + H2O + NAD+
-
25% activity with 2-oxopentanoate compared to 4-methyl-2-oxopentanoate
-
-
r
2-oxophenylacetic acid + NH3 + NADH + H+
2-aminophenylacetic acid + H2O + NAD+
-
-
-
-
r
3,3-dimethyl-2-oxobutanoate + NH3 + NADH + H+
L-tert-Leu + H2O + NAD+
-
-
-
-
r
3-methyl-2-oxobutanoate + NH3 + NADH + H+
L-valine + H2O + NAD+
-
-
-
-
r
3-methyl-2-oxopentanoate + NH3 + NADH
L-Ile + H2O + NAD+
-
64% activity with 3-methyl-2-oxopentanoate compared to 4-methyl-2-oxopentanoate
-
-
r
4-methyl-2-oxo-5,5,5-trifluoropentanoate + NH3 + NADH
2-amino-4-methyl-5,5,5-trifluoropentanoate + NAD+ + H2O
-
-
-
-
?
4-methyl-2-oxopentanoate + NH3 + NADH
L-Leu + H2O + NAD+
-
100% activity with 4-methyl-2-oxopentanoate
-
-
r
4-methylthio-2-oxobutyrate + NH3 + NADH
L-Met + H2O + NAD+
-
19% activity with 4-methylthio-2-oxobutyrate compared to 4-methyl-2-oxopentanoate
-
-
r
alpha-keto beta-methylvalerate + NH3 + NADH + H+
L-isoleucine + H2O + NAD+
yield: 95%
-
-
?
alpha-ketocaproate + NH3 + NADH + H+
L-norleucine + H2O + NAD+
yield: 80%
-
-
?
alpha-ketoisocaproate + NH3 + NADH + H+
L-leucine + H2O + NAD+
yield: 92.5%
-
-
?
alpha-ketoisovalerate + NH3 + NADH + H+
L-valine + H2O + NAD+
yield: 90%
-
-
?
alpha-ketovalerate + NH3 + NADH + H+
L-norvaline + H2O + NAD+
yield: 92%
-
-
?
amino(phenyl)acetate + H2O + NAD+
oxo(phenyl)acetate + NH3 + NADH + H+
-
-
-
-
r
DL-methionine + H2O + NAD+
3-methylthio-2-oxobutanoate + NH3 + NADH + H+
-
-
-
?
L-2-aminobutyrate + H2O + NAD+
2-oxobutanoate + NH3 + NADH
-
32% of the activity with L-Leu
-
-
?
L-arginine + H2O + NAD+
5-[(diaminomethyl)amino]-2-oxopentanoic acid + NH3 + NADH + H+
-
-
-
?
L-isoleucine + NH3 + NADH + H+
3-methyl-2-oxopentanoate + H2O + NAD+
-
-
-
?
L-Leu + H2O + 3-acetylpyridine-deamino-NAD+
4-methyl-2-oxopentanoate + NH3 + 3-acetylpyridine-deamino-NADH
-
as effective as NAD+
-
-
?
L-Leu + H2O + 3-acetylpyridine-NAD+
4-methyl-2-oxopentanoate + NH3 + 3-acetylpyridine-NADH
-
166% of the activity with NAD+
-
-
?
L-Leu + H2O + 3-pyridinealdehyde-NAD+
4-methyl-2-oxopentanoate + NH3 + 3-pyridinealdehyde-NADH
-
19% of the activity with NAD+
-
-
?
L-Leu + H2O + deamino-NAD+
4-methyl-2-oxopentanoate + NH3 + deamino-NADH
-
81% of the activity with NAD+
-
-
?
L-Leu + H2O + thionicotinamide-NAD+
4-methyl-2-oxopentanoate + NH3 + thionicotinamide-NADH
-
21% of the activity with NAD+
-
-
?
L-leucine + H2O + NADP+
4-methyl-2-oxopentanoate + NH3 + NADPH + H+
-
-
-
-
r
L-lysine + H2O + NAD+
6-amino-2-oxohexanoate + NH3 + NADH + H+
-
69% of the activity with L-leucine
-
-
?
L-norleucine + H2O + NAD+
? + NH3 + NADH
-
14% the activity with L-Leu, wild-type enzyme
-
-
?
L-norvaline + H2O + NAD+
2-ketovalerate + NH3 + NADH
-
56% the activity with L-Leu, wild-type enzyme
-
-
?
L-Phe + H2O + NAD+
phenylpyruvate + NH3 + NADH
-
no activity of wild-type enzyme activity with mutant enzymes A113G, A113G/V291L
-
-
?
L-S-methylcysteine + H2O + NAD+
3-methylthio-2-oxopropionate + NH3 + NADH
-
19% of the activity with L-Leu
-
-
?
L-valine + NH3 + NADH + H+
2-hydroxy-3-methylbutanoate + H2O + NAD+
-
-
-
?
phenylglyoxylate + NH3 + NADH + H+
amino(phenyl)acetate + H2O + NAD+
-
-
-
?
phenylpyruvate + NH3 + NADH
L-Phe + H2O + NAD+
-
15% of the activity with 2-ketoisocaproate, wild-type enzyme
-
-
?
S-methyl-L-cysteine + H2O + NAD+
3-methylthio-2-oxopropanoate + NH3 + NADH
-
19% the activity with L-Leu
-
-
?
trimethylpyruvic acid + NADH + NH3 + H+
L-tert-leucine + NAD+ + H2O
-
-
-
-
?
2-ketobutyrate + NH3 + NADH
L-2-aminobutyrate + H2O + NAD+
-
47% of the activity with 2-ketoisocaproate, wild-type enzyme
-
-
?
2-ketobutyrate + NH3 + NADH
L-2-aminobutyrate + H2O + NAD+
-
70% of the activity with 2-ketoisocaproate
-
-
?
2-ketobutyrate + NH3 + NADH
L-2-aminobutyrate + H2O + NAD+
-
70% of the activity with 2-ketoisocaproate
-
-
?
2-ketovalerate + NH3 + NADH
L-norvaline + H2O + NAD+
-
86% of the activity with 2-ketoisocaproate, wild-type enzyme
-
-
?
2-ketovalerate + NH3 + NADH
L-norvaline + H2O + NAD+
-
96% of the activity with 2-ketoisocaproate
-
-
?
2-oxo-3-methylbutanoate + NH3 + NADH + H+
L-2-amino-3-methylbutanoate + H2O + NAD+
-
-
-
?
2-oxo-3-methylbutanoate + NH3 + NADH + H+
L-2-amino-3-methylbutanoate + H2O + NAD+
-
-
-
?
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
-
-
-
?, r
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
-
-
?
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
-
-
?
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
-
-
-
r
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
-
-
?
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
-
-
?
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
-
-
-
-
r
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
Roseibium aggregatum
-
-
-
-
?
2-oxohexanoate + NH3 + NADH + H+
L-norleucine + H2O + NAD+
-
-
-
-
r
2-oxohexanoate + NH3 + NADH + H+
L-norleucine + H2O + NAD+
-
46% activity compared to 2-oxoisocaproate
-
-
r
2-oxopentanoate + NH3 + NADH + H+
L-2-aminopentanoate + H2O + NAD+
-
-
-
?
2-oxopentanoate + NH3 + NADH + H+
L-2-aminopentanoate + H2O + NAD+
-
-
-
?
2-oxopentanoate + NH3 + NADH + H+
L-2-aminopentanoate + H2O + NAD+
Roseibium aggregatum
-
-
-
-
?
2-oxopentanoate + NH3 + NADH + H+
L-2-aminopentanoate + H2O + NAD+
-
-
-
-
?
2-oxopentanoate + NH3 + NADH + H+
L-2-aminopentanoate + H2O + NAD+
Weizmannia coagulans NL01
-
-
-
-
?
3,3-dimethyl-2-oxobutanoate + NH3 + NADH
L-tert-Leu + H2O + NAD+
-
-
-
-
?
3,3-dimethyl-2-oxobutanoate + NH3 + NADH
L-tert-Leu + H2O + NAD+
-
-
-
?
3,3-dimethyl-2-oxobutanoate + NH3 + NADH
L-tert-Leu + H2O + NAD+
-
-
-
?
4-(methylsulfanyl)-2-oxobutanoate + NH3 + NADH + H+
L-methionine + H2O + NAD+
-
-
-
-
r
4-(methylsulfanyl)-2-oxobutanoate + NH3 + NADH + H+
L-methionine + H2O + NAD+
-
-
-
-
r
4-methyl-2-oxopentanoate + NH3 + NADH + H+
L-4-methyl-2-aminopentanoate + H2O + NAD+
-
-
-
?
4-methyl-2-oxopentanoate + NH3 + NADH + H+
L-4-methyl-2-aminopentanoate + H2O + NAD+
-
-
-
?
4-methyl-2-oxopentanoate + NH3 + NADH + H+
L-4-methyl-2-aminopentanoate + H2O + NAD+
Roseibium aggregatum
-
-
-
-
?
4-methyl-2-oxopentanoate + NH3 + NADH + H+
L-leucine + H2O + NAD+
-
-
-
-
r
4-methyl-2-oxopentanoate + NH3 + NADH + H+
L-leucine + H2O + NAD+
-
-
-
?
4-methyl-2-oxopentanoate + NH3 + NADH + H+
L-leucine + H2O + NAD+
-
-
-
?
4-methyl-2-oxopentanoate + NH3 + NADH + H+
L-leucine + H2O + NAD+
-
-
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
32% of the activity with L-Leu
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
-
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
-
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
41% of the activity with L-Leu
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
14% of the activity with L-Leu
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
5.7% activity wit L-2-aminobutanoate compared to L-Leu
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
5.7% activity wit L-2-aminobutanoate compared to L-Leu
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
11% of the activity with L-Leu
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
19% of the activity with L-Leu
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
-
-
-
?
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
-
-
r
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
-
-
-
r
L-Ala + H2O + NAD+
2-oxopropanoate + NH3 + NADH
-
10% of the activity with L-Leu
-
-
?
L-Ala + H2O + NAD+
2-oxopropanoate + NH3 + NADH
-
3.4% activity with L-Ala compared to L-Leu
-
-
r
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
72% of the activity with L-Leu
-
r
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
-
r
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
73% of the activity with L-Leu
-
-
r
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
54% of the activity with L-Leu, wild-type enzyme
-
-
?
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
-
r
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
58% of the activity with L-Leu
-
-
r
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
72.4% activity with L-Ile compared to L-Leu
-
-
r
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
72.4% activity with L-Ile compared to L-Leu
-
-
r
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
88% of the activity with L-Leu
-
r
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
72% of the activity with L-Leu
-
-
?
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
72% of the activity with L-Leu
-
-
?
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
-
?
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
-
-
-
r
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
69% of the activity with L-leucine
-
-
?
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
the equilibrium favors synthesis of L-Leu
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
the enzyme may have a function in spore germination
-
?
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
the enzyme may have a function in spore germination
-
?
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
-
?
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
100% activity with L-Leu
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
100% activity with L-Leu
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-leucine + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
-
-
-
?
L-leucine + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH + H+
Citrobacter freundii JK-91
-
-
-
-
?
L-leucine + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
-
-
-
r
L-leucine + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
-
-
?
L-leucine + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
-
-
?
L-leucine + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
-
-
-
?
L-leucine + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
-
-
-
r
L-leucine + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
-
-
?
L-leucine + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
-
-
-
?
L-leucine + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
-
-
-
?
L-leucine + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
-
-
-
?
L-Met + H2O + NAD+
4-methylthio-2-oxobutyrate + NH3 + NADH + H+
-
-
-
r
L-Met + H2O + NAD+
4-methylthio-2-oxobutyrate + NH3 + NADH + H+
-
-
-
r
L-Met + H2O + NAD+
4-methylthio-2-oxobutyrate + NH3 + NADH + H+
-
no activity
-
-
?
L-Met + H2O + NAD+
4-methylthio-2-oxobutyrate + NH3 + NADH + H+
-
0.7% the activity with L-Leu, wild-type enzyme
-
-
?
L-Met + H2O + NAD+
4-methylthio-2-oxobutyrate + NH3 + NADH + H+
-
1.2% activity with L-Met compared to L-Leu
-
-
r
L-Met + H2O + NAD+
4-methylthio-2-oxobutyrate + NH3 + NADH + H+
-
-
-
-
?
L-norleucine + H2O + NAD+
2-oxohexanoate + NH3 + NADH
-
7% of the activity with L-Leu
-
r
L-norleucine + H2O + NAD+
2-oxohexanoate + NH3 + NADH
-
-
-
-
?
L-norleucine + H2O + NAD+
2-oxohexanoate + NH3 + NADH
-
-
-
-
r
L-norleucine + H2O + NAD+
2-oxohexanoate + NH3 + NADH
-
74% of the activity with L-Leu
-
r
L-norleucine + H2O + NAD+
2-oxohexanoate + NH3 + NADH
-
15% of the activity with L-Leu
-
r
L-norvaline + H2O + NAD+
2-oxopentanoate + NH3 + NADH
-
36.8% activity with L-norvaline compared to L-Leu
-
-
r
L-norvaline + H2O + NAD+
2-oxopentanoate + NH3 + NADH
-
36.8% activity with L-norvaline compared to L-Leu
-
-
r
L-norvaline + H2O + NAD+
2-oxopentanoate + NH3 + NADH
-
7% the activity with L-Leu
-
-
?
L-norvaline + H2O + NAD+
2-oxopentanoate + NH3 + NADH
-
7% the activity with L-Leu
-
-
?
L-tert-Leu + H2O + NAD+
3,3-dimethyl-2-oxobutanoate + NH3 + NADH
-
-
-
-
?
L-tert-Leu + H2O + NAD+
3,3-dimethyl-2-oxobutanoate + NH3 + NADH
-
-
-
-
?
L-tert-leucine + H2O + NAD+
trimethylpyruvate + NH3 + NADH + H+
-
-
-
-
r
L-tert-leucine + H2O + NAD+
trimethylpyruvate + NH3 + NADH + H+
Roseibium aggregatum
-
-
-
-
?
L-Val + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH
-
59% of the activity with L-Leu
-
r
L-Val + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH
-
-
-
-
?
L-Val + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH
-
98% of the activity with L-Leu
-
r
L-Val + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH
-
39% of the activity with L-Leu, wild-type enzyme
-
-
?
L-Val + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH
-
-
-
-
?
L-Val + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH
-
-
-
-
r
L-Val + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH
-
74% of the activity with L-Leu
-
-
?
L-Val + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH
-
93% of the activity with L-Leu
-
r
L-Val + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH
-
59% of the activity with L-Leu
-
-
?
L-Val + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH
-
59% of the activity with L-Leu
-
-
?
L-Val + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH
-
-
-
r
L-Val + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH
-
-
-
r
L-valine + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
-
-
-
r
L-valine + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
69% of the activity with L-leucine
-
-
?
L-valine + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
-
-
-
r
oxo(phenyl)acetate + NH3 + NADH + H+
amino(phenyl)acetate + H2O + NAD+
-
-
-
?
oxo(phenyl)acetate + NH3 + NADH + H+
amino(phenyl)acetate + H2O + NAD+
-
-
-
-
r
oxo(phenyl)acetate + NH3 + NADH + H+
amino(phenyl)acetate + H2O + NAD+
Roseibium aggregatum
-
-
-
-
?
pyruvate + NH3 + NADH + H+
L-alanine + H2O + NAD+
-
-
-
?
trimethylpyruvate + NH3 + NADH + H+
L-tert-leucine + H2O + NAD+
-
-
-
?
trimethylpyruvate + NH3 + NADH + H+
L-tert-leucine + H2O + NAD+
-
-
-
?
trimethylpyruvate + NH3 + NADH + H+
L-tert-leucine + H2O + NAD+
-
-
-
?
trimethylpyruvate + NH3 + NADH + H+
L-tert-leucine + H2O + NAD+
-
-
-
?
trimethylpyruvate + NH3 + NADH + H+
L-tert-leucine + H2O + NAD+
-
-
-
?
trimethylpyruvate + NH3 + NADH + H+
L-tert-leucine + H2O + NAD+
-
-
-
?
trimethylpyruvate + NH3 + NADH + H+
L-tert-leucine + H2O + NAD+
-
-
-
?
trimethylpyruvate + NH3 + NADH + H+
L-tert-leucine + H2O + NAD+
-
-
-
-
r
trimethylpyruvate + NH3 + NADH + H+
L-tert-leucine + H2O + NAD+
Roseibium aggregatum
-
-
-
-
?
additional information
?
-
-
enzyme liberates ammonium ions from branched chain amino acids when supplied as the sole nitrogen source. It synthesizes from L-Ile, L-Leu, L-Val the branched chain alpha-oxo acids which are precursors of branched chain fatty acid biosynthesis
-
-
?
additional information
?
-
-
no substrates: D-leucine, L-arginine, aspartate, glycine, L-threonine
-
-
?
additional information
?
-
Citrobacter freundii JK-91
-
no substrates: D-leucine, L-arginine, aspartate, glycine, L-threonine
-
-
?
additional information
?
-
-
substrate specificity of the chimeric enzyme consisting of an amino-terminal domain of phenylalanine dehydrogenase and a carboxy-terminal domain of leucine dehydrogenase
-
-
?
additional information
?
-
-
reductive amination proceeds through a sequential ordered ternary-binary mechanism
-
-
?
additional information
?
-
-
antitumor activity, the enzyme is highly inhibitory to Ehrlich ascites carcinoma cells
-
-
?
additional information
?
-
-
no activity with L-Cys, L-Phe, 2-oxo-3-phenylpropionic acid, 3-(2'-nitrophenyl)-2-oxopropionic acid, and 3-(3'-indolyl)-2-oxopropionic acid
-
-
?
additional information
?
-
-
no activity with L-Cys, L-Phe, 2-oxo-3-phenylpropionic acid, 3-(2'-nitrophenyl)-2-oxopropionic acid, and 3-(3'-indolyl)-2-oxopropionic acid
-
-
?
additional information
?
-
-
synthesis of (S)-1-cyclopropyl-2-methoxyethanamine starting from methylcyclopropyl ketone. Permanganate oxidation of the ketone gives cyclopropylglyoxylic acid, which is converted to (S)-cyclopropylglycine by reductive amination using leucine dehydrogenase from Thermoactinomyces intermedius with NADH cofactor recycling by formate dehydrogenase from Pichia pastoris
-
-
?
additional information
?
-
-
comparing extract activities with 5 mM 2-oxoisovalerate (3282 U/mL) versus 5 mM cyclopropylglyoxylic acid (86 U/mL), leucine dehydrogenase is 38fold more active with the natural substrate
-
-
?
