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1.4.1.9: leucine dehydrogenase

This is an abbreviated version!
For detailed information about leucine dehydrogenase, go to the full flat file.

Word Map on EC 1.4.1.9

Reaction

L-leucine
+
H2O
 +
NAD+
=
4-methyl-2-oxopentanoate
 +
NH3
 +
NADH
 +
H+

Synonyms

BCD, dehydrogenase, leucine, L-leucine dehydrogenase, L-leucine:NAD+ oxidoreductase, deaminating, LeuDH

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.9 leucine dehydrogenase

Specific Activity

Specific Activity on EC 1.4.1.9 - leucine dehydrogenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.074
-
strain DSM 3, pH 10.5, 25°C
0.88
-
pH 7.0, temperature not specified in the publication
10.4
-
mutant M347G/Q358N, pH 7.5, 37°C
11.94
-
mutant Q358N, pH 7.5, 37°C
12.19
-
enzyme after Ni-NTA agaose gel purification, using L-Leu as substrate
122
-
recombinant enzyme
13099
Roseibium aggregatum
-
substrate 2-aminopentanoate, wild-type, pH 8, 30°C
133
-
substrate L-leucine, presence of 0.5 M KCl, pH 10.5, 25°C
1338
-
pH 8, 50°C
1410
Roseibium aggregatum
-
substrate oxo(phenyl)acetate, wild-type, pH 8, 30°C
152
-
substrate L-leucine, presence of 0.5 M NaCl, pH 10.5, 25°C
15871
Roseibium aggregatum
-
substrate 2-oxobutanoate, wild-type, pH 8, 30°C
1710
Roseibium aggregatum
-
substrate oxo(phenyl)acetate, mutant D153N/H191N, pH 8, 30°C
1743
Roseibium aggregatum
-
substrate 2-oxopropanoate, wild-type, pH 8, 30°C
183
-
substrate L-leucine, pH 10.5, 25°C
19609
Roseibium aggregatum
-
substrate 4-methyl-2-oxopentanoate, mutant D153N/H191N, pH 8, 30°C
2.6
-
wild-type, pH 7.5, 37°C
2217
Roseibium aggregatum
-
substrate 2-oxopropanoate, mutant D153N/H191N, pH 8, 30°C
23654
Roseibium aggregatum
-
substrate 2-aminopentanoate, mutant D153N/H191N, pH 8, 30°C
275
pH 9, 30°C, substrate L-leucine
31256
Roseibium aggregatum
-
substrate 2-oxobutanoate, mutant D153N/H191N, pH 8, 30°C
320
-
pH not specified in the publication, temperature not specified in the publication
327
-
substrate L-leucine, pH 10.5, 60°C
51
-
pH 9.5, 30°C
53.8
-
purified enzyme, pH 10.5, 25°C
556.6
Roseibium aggregatum
-
substrate trimethylpyruvate, wild-type, pH 8, 30°C
6.27
-
enzyme from cell-free extract, using L-Leu as substrate
8.88
-
mutant M374G, pH 7.5, 37°C
9046
Roseibium aggregatum
-
substrate 4-methyl-2-oxopentanoate, wild-type, pH 8, 30°C
additional information