Literature summary for 1.4.1.9 extracted from

Nagata, S.; Tanizawa, K.; Esaki, N.; Sakamoto, Y.; Ohshima, T.; Tanaka, H.; Soda, K.
Gene cloning and sequence determination of leucine dehydrogenase from Bacillus stearothermophilus and structural comparison with other NAD(P)+-dependent dehydrogenases (1988), Biochemistry, 27, 9056-9062.

Search for more literature for 1.4.1.9

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46903	-	x * 46903, calculation from nucleotide sequence	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
112	-	-	Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Leu + H2O + NAD+	-	Geobacillus stearothermophilus	4-methyl-2-oxopentanoate + NH3 + NADH	-	r

Subunits

Subunits	Comment	Organism
?	x * 46903, calculation from nucleotide sequence	Geobacillus stearothermophilus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Geobacillus stearothermophilus
NADH	-	Geobacillus stearothermophilus

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Release 2023.1 (January 2023)