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0.0018 - 0.003
12'-apo-beta-carotenal
-
0.0006 - 0.00062
13-cis-retinal
0.005 - 0.012
14'-apo-beta-carotenal
0.095
3-deoxyglucosone
-
partially purified recombinant ALDH1A1 at pH 7.1 and in the presence of 1 mM NAD
0.00225 - 0.0087
9-cis-retinal
0.0002 - 0.015
all-trans-retinal
0.0032
all-trans-retinaldehyde
-
-
0.003
all-trans-retinol
-
-
0.0003 - 0.103
benzaldehyde
additional information
additional information
-
0.0018
12'-apo-beta-carotenal
isoform ALDH1A2, at pH 8.0 and 37°C
-
0.003
12'-apo-beta-carotenal
isoform ALDH1A3, at pH 8.0 and 37°C
-
0.0034
13-cis retinal
-
0.0034
13-cis retinal
isoform RALDH4, in 100 mM HEPES buffer, pH 8.5, at 37°C
0.0006
13-cis-retinal
-
-
0.00062
13-cis-retinal
-
recombinant isozyme type-2, pH 9.0, 25°C
0.005
14'-apo-beta-carotenal
isoform ALDH1A2, at pH 8.0 and 37°C
0.012
14'-apo-beta-carotenal
isoform ALDH1A1, at pH 8.0 and 37°C
0.0063
2,4-decadienal
pH 8.5, 25°C, recombinant enzyme
0.0063
2,4-decadienal
-
wild type enzyme ALDH1A2, at pH 8.5 and 25°C
0.003
9-cis retinal
-
0.003
9-cis retinal
isoform RALDH4, in 100 mM HEPES buffer, pH 8.5, at 37°C
0.00225
9-cis-retinal
-
recombinant isozyme type-2, pH 9.0, 25°C
0.0036
9-cis-retinal
-
recombinant isozyme type-1, pH 7.5, 25°C
0.0087
9-cis-retinal
-
25°C
0.0087
9-cis-retinal
-
recombinant wild-type enzyme, pH 7.2, 25°C
0.014
acetaldehyde
-
recombinant wild-type enzyme, pH 8.5, 25°C
0.019
acetaldehyde
-
50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.01 mM Zn2+, 12 mM acetaldehyde, 0.015-0.024 mM NAD+
0.024
acetaldehyde
-
50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.02 Mn2+, 12 mM acetaldehyde, 0.015-0.024 mM NAD+
0.027
acetaldehyde
-
50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.03 mM Ca2+, 12 mM acetaldehyde, 0.015-0.024 mM NAD+
0.028
acetaldehyde
-
50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.1 mM Mg2+, 12 mM acetaldehyde, 0.015-0.024 mM NAD+
0.031
acetaldehyde
-
recombinant mutant L459F/N460G, pH 8.5, 25°C
0.055
acetaldehyde
-
50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 12 mM acetaldehyde, 0.015-0.024 mM NAD+
1.4
acetaldehyde
-
50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.3 mM Ca2+, 1-12 mM acetaldehyde, 0.33 mM NAD+
1.8
acetaldehyde
-
50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.1 mM Mg2+, 1-12 mM acetaldehyde, 0.33 mM NAD+
2
acetaldehyde
-
50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.02 mM Mn2+, 1-12 mM acetaldehyde, 0.33 mM NAD+
2.1
acetaldehyde
-
50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.01 mM Zn2+, 1-12 mM acetaldehyde, 0.33 mM NAD+
3.4
acetaldehyde
-
50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 1-12 mM acetaldehyde, 0.33 mM NAD+
0.0039
all-trans retinal
-
0.0039
all-trans retinal
isoform RALDH3, in 10 mM Tris-HCl buffer, pH 8.5, at 37°C
0.0002
all-trans-retinal
wild-type
0.0002
all-trans-retinal
F471L mutant
0.00066
all-trans-retinal
-
recombinant isozyme type-2, pH 9.0, 25°C
0.0007
all-trans-retinal
-
-
0.0017
all-trans-retinal
-
-
0.002
all-trans-retinal
pH 8.5, 25°C, recombinant enzyme
0.0081
all-trans-retinal
pH 8.5, 25°C, recombinant wild-type ALDH1A1 enzyme
0.0086
all-trans-retinal
pH 8.5, 25°C, recombinant mutant K127A
0.0089
all-trans-retinal
pH 8.5, 25°C, recombinant mutant Y296A
0.01
all-trans-retinal
-
25°C
0.01
all-trans-retinal
-
recombinant wild-type enzyme, pH 7.2, 25°C
0.011
all-trans-retinal
pH 8.5, 25°C, recombinant mutant N120A
0.0116
all-trans-retinal
-
recombinant isozyme type-1, pH 7.5, 25°C
0.015
all-trans-retinal
pH 8.5, 25°C, recombinant mutant Y296V
0.0003
benzaldehyde
-
-
0.103
benzaldehyde
wild-type
0.0029
citral
pH 8.5, 25°C, recombinant enzyme
0.0029
citral
-
wild type enzyme ALDH1A2, at pH 8.5 and 25°C
0.0003
decanal
-
-
0.001
decanal
-
recombinant wild-type enzyme and mutant L459F/N460G, pH 8.5, 25°C
0.0064
decanal
pH 8.5, 25°C, recombinant enzyme
0.0064
decanal
-
wild type enzyme ALDH1A2, at pH 8.5 and 25°C
0.01
decanal
-
wild type enzyme ALDH1A1, at pH 8.5 and 25°C
0.01
decanal
pH 8.5, 25°C, recombinant wild-type ALDH1A1 enzyme
0.0003
hexanal
-
-
0.005
hexanal
-
recombinant wild-type enzyme, pH 8.5, 25°C
0.008
hexanal
pH 8.0, 25°C, recombinant enzyme
0.008
hexanal
-
recombinant mutant L459F/N460G, pH 8.5, 25°C
0.023
hexanal
pH 8.5, 25°C, recombinant enzyme
0.023
hexanal
-
wild type enzyme ALDH1A2, at pH 8.5 and 25°C
0.022
NAD+
-
standard deviation 0.004 mM
0.067
NAD+
-
recombinant wild-type enzyme, pH 8.5, 25°C
0.085
NAD+
-
partially purified recombinant ALDH1A1 at pH 7.1 and in the presence of 0.1 mM 3-deoxyglucosone
0.166
NAD+
microsomes, high-fate diet rats, pH and temperature not specified in the publication
0.1822
NAD+
microsomes, control rats, pH and temperature not specified in the publication
0.2269
NAD+
cytosol, control rats, pH and temperature not specified in the publication
0.2424
NAD+
cytosol, high-fate diet rats, pH and temperature not specified in the publication
0.39
NAD+
-
recombinant mutant L459F/N460G, pH 8.5, 25°C
0.0003
octanal
-
-
0.0009
octanal
F471L mutant
0.005
octanal
-
recombinant mutant L459F/N460G, pH 8.5, 25°C
0.01
octanal
-
recombinant wild-type enzyme, pH 8.5, 25°C
0.0007
retinal
cellular retinol-binding protein-bound retinal has lower Km of 0.0002 mM
0.001
retinal
-
50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.03 mM retinal, 0.056-0.12 mM NAD+
0.002
retinal
-
wild type enzyme ALDH1A2, at pH 8.5 and 25°C
0.0044
retinal
-
50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.5 mM Ca2+, 0.03 mM retinal, 0.056-0.12 mM NAD+
0.0046
retinal
-
50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.04 mM Mn2+, 0.03 mM retinal, 0.056-0.12 mM NAD+
0.006
retinal
-
50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.3 mM Mg2+, 0.03 mM retinal, 0.056-0.12 mM NAD+
0.0064
retinal
-
50 mM Tris/HCl buffer, pH 8.0, 3.3 mM pyrazole, 100 mM KCl, 1 mM dithiothreitol, 0.03 mM Zn2+ 0.03 mM retinal, 0.056-0.12 mM NAD+
0.0081
retinal
-
wild type enzyme ALDH1A1, at pH 8.5 and 25°C
0.0085
retinal
-
retinal bovine serum albumin, Km is 25 times lower than the Km for acetaldehyde
0.0086
retinal
-
mutant enzyme K127A, at pH 8.5 and 25°C
0.0089
retinal
-
mutant enzyme Y296A, at pH 8.5 and 25°C
0.0104
retinal
-
retinal-Tween-80
0.011
retinal
-
mutant enzyme N120A, at pH 8.5 and 25°C
0.015
retinal
-
mutant enzyme Y296V, at pH 8.5 and 25°C
0.0807
retinal
isoform RALDH2, at pH 8.2 and 25°C
additional information
additional information
-
kinetic analysis, isozyme type-2
-
additional information
additional information
-
kinetics, recombinant wild-type and chimeric mutant enzymes
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics and pre-steady-state kinetics
-
additional information
additional information
Michaelis-Menten kinetics and pre-steady-state kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics and pre-steady-state kinetics
-