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Literature summary for 1.2.1.36 extracted from
- Kinetic characterization of recombinant mouse retinal dehydrogenase types 3 and 4 for retinal substrates (2009), Biochim. Biophys. Acta, 1790, 1660-1664.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells as His-tagged enzyme | Mus musculus |
| into the vector pET28a for expression in Escherichia coli BL21DE3 cells | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| beta-ionone | potent inhibitor of isoform RALDH4 activity, at 0.002 mM concentration 9-cis and 13-cis retinal oxidation is reduced by 53% and 63%, respectively | Mus musculus |  |
| Mg2+ | 4 mM Mg2+ inhibits 9-cis retinal oxidation by isoform RALDH4 by 30% | Mus musculus | |
| additional information | beta-ionone has no effect on isoform RALDH3 activity | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
9-cis retinal | - |
Mus musculus | |
| 0.003 | - |
9-cis retinal | isoform RALDH4, in 100 mM HEPES buffer, pH 8.5, at 37°C | Mus musculus | |
| 0.0034 | - |
13-cis retinal | - |
Mus musculus | |
| 0.0034 | - |
13-cis retinal | isoform RALDH4, in 100 mM HEPES buffer, pH 8.5, at 37°C | Mus musculus | |
| 0.0039 | - |
all-trans retinal | - |
Mus musculus | |
| 0.0039 | - |
all-trans retinal | isoform RALDH3, in 10 mM Tris-HCl buffer, pH 8.5, at 37°C | Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Mus musculus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activity is slightly activated by magnesium at higher concentrations | Mus musculus | |
| Mg2+ | magnesium dramatically activated 13-cis retinal oxidation without significantly affecting 9-cis retinal oxidation | Mus musculus | |
| Mg2+ | Mg2+ activates 13-cis retinal oxidation by isoform RALDH4 by 3fold, does not significantly influence 9-cis retinal oxidation, and slightly activates isoform RALDH3 activity, owever, magnesium at lower concentration has no effect on RALDH4 activity | Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
SDS-PAGE | Mus musculus |
| 55000 | - |
determined by SDS-PAGE | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| retinal + NAD+ + H2O | Mus musculus | - |
retinoate + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
| Mus musculus | Q9JHW9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Mus musculus |
| on a Ni-NTA column | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | adult mouse | Mus musculus | - |
| liver | adult mouse | Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 13-cis-retinal + NAD+ + H2O | - |
Mus musculus | 13-cis-retinoate + NADH + H+ | - |
? | |
| 13-cis-retinal + NAD+ + H2O | isoform RALDH4 oxidizes 13-cis retinal with lower catalytic efficiency than 9-cis retinal | Mus musculus | 13-cis-retinoate + NADH + H+ | - |
? | |
| 9-cis-retinal + NAD+ + H2O | - |
Mus musculus | 9-cis-retinoate + NADH + H+ | - |
? | |
| 9-cis-retinal + NAD+ + H2O | isoform RALDH4 catalyzes 9-cis retinal oxidation with 3fold higher efficiency than 13-cis retinal | Mus musculus | 9-cis-retinoate + NADH + H+ | - |
? | |
| all-trans retinal + NAD+ + H2O | - |
Mus musculus | retinoate + NADH + H+ | - |
? | |
| all-trans retinal + NAD+ + H2O | RALDH3 oxidizes all-trans retinal with high catalytic efficiency | Mus musculus | all-trans-retinoate + NADH + H+ | - |
? | |
| additional information | show no activity with either 9-cis or 13-cis retinal | Mus musculus | ? | - |
? | |
| additional information | isoform RALDH3 does not show activity for either 9-cis or 13-cis retinal substrates, isoform RALDH4 is inactive for all-trans retinal substrate | Mus musculus | ? | - |
? | |
| retinal + NAD+ + H2O | - |
Mus musculus | retinoate + NADH + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RALDH3 | - |
Mus musculus |
| RALDH3 | isoform | Mus musculus |
| Raldh4 | - |
Mus musculus |
| Raldh4 | isoform | Mus musculus |
| retinal dehydrogenase 3 | - |
Mus musculus |
| retinal dehydrogenase 4 | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
activity assay | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
activity assay | Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Mus musculus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00032 | - |
beta-ionone | isoform RALDH4, using 13-cis retinal as substrate, in 100 mM HEPES buffer, pH 8.5, at 37°C | Mus musculus | |
| 0.00061 | - |
beta-ionone | isoform RALDH4, using 9-cis retinal as substrate, in 100 mM HEPES buffer, pH 8.5, at 37°C | Mus musculus | |
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Release 2023.1 (January 2023)
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