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Literature summary for 1.2.1.36 extracted from

  • Collard, F.; Vertommen, D.; Fortpied, J.; Duester, G.; Van Schaftingen, E.
    Identification of 3-deoxyglucosone dehydrogenase as aldehyde dehydrogenase 1A1 (retinaldehyde dehydrogenase 1) (2007), Biochimie, 89, 369-373.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
degradation ALDH1A1 appears to be the major if not the only enzyme responsible for the oxidation of 3-deoxyglucosone to 2-keto-3-deoxygluconate Mus musculus
degradation urinary excretion of 2-keto-3-deoxygluconate amounts to 16.7 micromol/g creatinine in humans, indicating that 3-deoxyglucosone may be quantitatively a more important substrate than retinaldehyde for ALDH1A1 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
ligated into a pCMV5 expression vector and overexpressed in HEK-293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information 3-deoxyglucosone dehydrogenase activity is undetectable in tissues from ALDH1A1-/- mice Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
benzaldehyde
-
Homo sapiens
0.085
-
NAD+ partially purified recombinant ALDH1A1 at pH 7.1 and in the presence of 0.1 mM 3-deoxyglucosone Homo sapiens
0.095
-
3-deoxyglucosone partially purified recombinant ALDH1A1 at pH 7.1 and in the presence of 1 mM NAD Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
sequence analysis Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration, more than 400fold with a yield of about 1%, to near homogeneity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Mus musculus
-
erythrocyte
-
Homo sapiens
-
liver highest activity Mus musculus
-
lung intermediate activity Mus musculus
-
additional information no activity is detectable in brain, thymus, spleen, kidneys, skeletal muscle and heart Mus musculus
-
testis intermediate activity Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-deoxyglucosone + NAD+ + H2O
-
Mus musculus 2-keto-3-deoxygluconate + NADH + H+
-
?
3-deoxyglucosone + NAD+ + H2O
-
Homo sapiens 2-keto-3-deoxygluconate + NADH + H+
-
?
benzaldehyde + NAD+ + H2O
-
Homo sapiens benzoate + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
aldehyde dehydrogenase 1A1
-
Mus musculus
aldehyde dehydrogenase 1A1
-
Homo sapiens
ALDH1A1
-
Mus musculus
ALDH1A1
-
Homo sapiens
retinaldehyde dehydrogenase 1
-
Mus musculus
retinaldehyde dehydrogenase 1
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7.1 9
-
Homo sapiens