Literature summary for 1.2.1.36 extracted from

Bordelon, T.; Montegudo, S.K.; Pakhomova, S.; Oldham, M.L.; Newcomer, M.E.
A disorder to order transition accompanies catalysis in retinaldehyde dehydrogenase type II (2004), J. Biol. Chem., 279, 43085-43091.

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of wild-type and mutant isozyme type-2 in Escherichia coli strain Bl21(DE3)	Mus musculus

Crystallization (Commentary)

Crystallization (Comment)	Organism
recombinant isozyme type-2 mutant L459F/N460G in complex with NAD+ and in absence of substrate, hanging drop vapour diffusion method, 7.6 mg/ml enzyme with 2 mM NAD+ and about 14 mg/ml retinol, 0.002 ml protein solution plus equal volume of well solution containing 7% PEG 6000, 2% 2-methyl-2,4-pentanediol, and 0.1 M Tris, pH 7.5, X-ray diffraction structure determination and analysis at 3.3 A resolution	Mus musculus

Crystallization (Comment)

Organism

recombinant isozyme type-2 mutant L459F/N460G in complex with NAD+ and in absence of substrate, hanging drop vapour diffusion method, 7.6 mg/ml enzyme with 2 mM NAD+ and about 14 mg/ml retinol, 0.002 ml protein solution plus equal volume of well solution containing 7% PEG 6000, 2% 2-methyl-2,4-pentanediol, and 0.1 M Tris, pH 7.5, X-ray diffraction structure determination and analysis at 3.3 A resolution

Mus musculus

Protein Variants

Protein Variants	Comment	Organism
C301A	site-directed mutagenesis, catalytic residue mutation, reduced activity compared to the wild-type enzyme	Mus musculus
L459F/N460G	site-directed mutagenesis, inactive mutant, still able to bind the cofactor NAD+	Mus musculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.001	-	decanal	recombinant wild-type enzyme and mutant L459F/N460G, pH 8.5, 25°C	Mus musculus
0.005	-	octanal	recombinant mutant L459F/N460G, pH 8.5, 25°C	Mus musculus
0.005	-	hexanal	recombinant wild-type enzyme, pH 8.5, 25°C	Mus musculus
0.008	-	hexanal	recombinant mutant L459F/N460G, pH 8.5, 25°C	Mus musculus
0.01	-	octanal	recombinant wild-type enzyme, pH 8.5, 25°C	Mus musculus
0.014	-	acetaldehyde	recombinant wild-type enzyme, pH 8.5, 25°C	Mus musculus
0.031	-	acetaldehyde	recombinant mutant L459F/N460G, pH 8.5, 25°C	Mus musculus
0.067	-	NAD+	recombinant wild-type enzyme, pH 8.5, 25°C	Mus musculus
0.39	-	NAD+	recombinant mutant L459F/N460G, pH 8.5, 25°C	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	isozyme type-2	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type and mutant isozyme type-2 from Escherichia coli strain Bl21(DE3), no usage of His-tag since it may influence the enzyme activity	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
acetaldehyde + NAD+ + H2O	-	Mus musculus	acetate + NADH + H+	-	?
decanal + NAD+ + H2O	-	Mus musculus	decanoate + NADH	-	?
hexanal + NAD+ + H2O	-	Mus musculus	hexanoate + NADH + H+	-	?
additional information	substrate specificities of wild-type isozyme type-2 and mutant L459F/N460G	Mus musculus	?	-	?
octanal + NAD+ + H2O	-	Mus musculus	octanoate + NADH	-	?

Synonyms

Synonyms	Comment	Organism
RALDH2	-	Mus musculus
retinaldehyde dehydrogenase type II	-	Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Mus musculus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	assay at	Mus musculus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Mus musculus