Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C41(DE3) cells | Homo sapiens |
expressed in Escherichia coli C41(DE3) cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
K127A | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
N120A | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
Y296A | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
Y296V | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
retinal | wild type enzyme ALDH1A2, at pH 8.5 and 25°C | Rattus norvegicus | |
0.0029 | - |
citral | wild type enzyme ALDH1A2, at pH 8.5 and 25°C | Rattus norvegicus | |
0.0063 | - |
2,4-decadienal | wild type enzyme ALDH1A2, at pH 8.5 and 25°C | Rattus norvegicus | |
0.0064 | - |
decanal | wild type enzyme ALDH1A2, at pH 8.5 and 25°C | Rattus norvegicus | |
0.0081 | - |
retinal | wild type enzyme ALDH1A1, at pH 8.5 and 25°C | Homo sapiens | |
0.0086 | - |
retinal | mutant enzyme K127A, at pH 8.5 and 25°C | Homo sapiens | |
0.0089 | - |
retinal | mutant enzyme Y296A, at pH 8.5 and 25°C | Homo sapiens | |
0.01 | - |
decanal | wild type enzyme ALDH1A1, at pH 8.5 and 25°C | Homo sapiens | |
0.011 | - |
retinal | mutant enzyme N120A, at pH 8.5 and 25°C | Homo sapiens | |
0.015 | - |
retinal | mutant enzyme Y296V, at pH 8.5 and 25°C | Homo sapiens | |
0.023 | - |
hexanal | wild type enzyme ALDH1A2, at pH 8.5 and 25°C | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
retinal + NAD+ + H2O | Homo sapiens | - |
retinoate + NADH + H+ | - |
? | |
retinal + NAD+ + H2O | Rattus norvegicus | - |
retinoate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel-Sepharose column chromatography and Q-Sepharose column chromatography | Homo sapiens |
nickel-Sepharose column chromatography and Q-Sepharose column chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
testis | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-decadienal + NAD+ + H2O | - |
Homo sapiens | 2,4-decadienoate + NADH + H+ | - |
? | |
2,4-decadienal + NAD+ + H2O | - |
Rattus norvegicus | 2,4-decadienoate + NADH + H+ | - |
? | |
citral + NAD+ + H2O | - |
Homo sapiens | (2E)-3,7-dimethylocta-2,6-dienoic acid + NADH + H+ | - |
? | |
citral + NAD+ + H2O | - |
Rattus norvegicus | (2E)-3,7-dimethylocta-2,6-dienoic acid + NADH + H+ | - |
? | |
decanal + NAD+ + H2O | - |
Homo sapiens | decanoate + NADH + H+ | - |
? | |
decanal + NAD+ + H2O | - |
Rattus norvegicus | decanoate + NADH + H+ | - |
? | |
hexanal + NAD+ + H2O | - |
Homo sapiens | hexanoate + NADH + H+ | - |
? | |
hexanal + NAD+ + H2O | - |
Rattus norvegicus | hexanoate + NADH + H+ | - |
? | |
retinal + NAD+ + H2O | - |
Homo sapiens | retinoate + NADH + H+ | - |
? | |
retinal + NAD+ + H2O | - |
Rattus norvegicus | retinoate + NADH + H+ | - |
? | |
retinol + NAD+ + H2O | - |
Homo sapiens | ? + NADH + H+ | - |
? | |
retinol + NAD+ + H2O | - |
Rattus norvegicus | ? + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ALDH1A1 | - |
Homo sapiens |
Aldh1a2 | - |
Rattus norvegicus |
NAD-dependent retinal dehydrogenase | - |
Rattus norvegicus |
NAD-dependent retinal dehydrogenases | - |
Homo sapiens |
RalDH1 | - |
Homo sapiens |
RALDH2 | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
retinal | mutant enzyme Y296V, at pH 8.5 and 25°C | Homo sapiens | |
0.017 | - |
retinal | mutant enzyme Y296A, at pH 8.5 and 25°C | Homo sapiens | |
0.03 | - |
retinal | mutant enzyme K127A, at pH 8.5 and 25°C | Homo sapiens | |
0.03 | - |
retinal | mutant enzyme N120A, at pH 8.5 and 25°C | Homo sapiens | |
0.07 | - |
retinal | wild type enzyme ALDH1A1, at pH 8.5 and 25°C | Homo sapiens | |
0.1 | - |
retinal | wild type enzyme ALDH1A2, at pH 8.5 and 25°C | Rattus norvegicus | |
0.1 | - |
citral | wild type enzyme ALDH1A2, at pH 8.5 and 25°C | Rattus norvegicus | |
0.15 | - |
2,4-decadienal | wild type enzyme ALDH1A2, at pH 8.5 and 25°C | Rattus norvegicus | |
2 | - |
hexanal | wild type enzyme ALDH1A2, at pH 8.5 and 25°C | Rattus norvegicus | |
2.2 | - |
decanal | wild type enzyme ALDH1A2, at pH 8.5 and 25°C | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens | |
NAD+ | - |
Rattus norvegicus |