1.14.99.29: deoxyhypusine monooxygenase
This is an abbreviated version!
For detailed information about deoxyhypusine monooxygenase, go to the full flat file.
Word Map on EC 1.14.99.29
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1.14.99.29
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eif-5a
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spermidine
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hypusine-containing
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gc7
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mimosine
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ciclopirox
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nepsilon-4-amino-2-hydroxybutyllysine
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drug development
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deferiprone
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eif5a-1
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polyamine-derived
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4-aminobutyl
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diiron
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n1-guanyl-1,7-diaminoheptane
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heat-repeats
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heat-like
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peroxo
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deoxyhypusine-containing
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medicine
- 1.14.99.29
-
eif-5a
- spermidine
-
hypusine-containing
- gc7
- mimosine
- ciclopirox
-
nepsilon-4-amino-2-hydroxybutyllysine
- drug development
- deferiprone
-
eif5a-1
-
polyamine-derived
-
4-aminobutyl
-
diiron
- n1-guanyl-1,7-diaminoheptane
-
heat-repeats
-
heat-like
-
peroxo
-
deoxyhypusine-containing
- medicine
Reaction
Synonyms
deoxyhypusine hydroxylase, deoxyhypusine hydroxylase homologue nero, deoxyhypusine synthase/hydroxylase, deoxyhypusyl hydroxylase, DOHH, DOOH, hDOHH, Lia1, More, nero, oxygenase, deoxyhypusine di-
ECTree
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Purification
Purification on EC 1.14.99.29 - deoxyhypusine monooxygenase
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GSH-Sepharose resin column chromatography and Superdex 200 gel filtration
native plasmodial DOHH by anion exchange chromatography and nickel affinity chromatography
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performed by nickel-chelate affinity chromatography under native conditions
recombinant GST-tagged DOHH by glutathione affinity chromatography
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recombinant GST-tagged DOHH by glutathione affinity chromatography. Recombinant His-tagged hypusine pathway enzymes from Escherichia coli by anion exchange and nickel affinity chromatography
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recombinant GST-tagged Lia1 from Escherichia coli strain BL21(DE3) by two steps of glutathione affinity chromatography and gel filtration. Separation of iron-free and iron-bound forms by gel filtration and native electrophoresis
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recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, the tag is cleaved off by thrombin
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recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain JM109 by glutathione affinity chromatography, the GST-tag is cleaved off
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affiniity chromatography