5.2.1.5: linoleate isomerase

This is an abbreviated version!
For detailed information about linoleate isomerase, go to the full flat file.

Reaction

Synonyms

C12 isomerase, FG02668.1, Isomerase, linoleate, IV39_GL000089, LA isomerase, LA-I, LAI, LAI12, Linoleate DELTA12-cis,DELTA11-trans isomerase, linoleic acid DELTA12-cis,DELTA11-trans-isomerase, Linoleic acid isomerase, PAI

ECTree

     5 Isomerases

         5.2 cis-trans-Isomerases

             5.2.1 cis-trans Isomerases (only sub-subclass identified to date)

                5.2.1.5 linoleate isomerase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
13	Activating Compound
14	AA Sequence
12	Application
1	CAS Registry Number
3	Cloned(Commentary)
1	Cofactor
2	Expression
4	General Information
2	General Stability
48	Inhibitors
10	KM Value [mM]
6	Localization
1	Metals/Ions
4	Molecular Weight [Da]
15	Natural Substrates/ Products (Substrates)
44	Organism
1	Pathway
3	pH Optimum
1	pH Range
1	pH Stability
1	pI Value
5	Purification (Commentary)
4	Reaction
2	Reaction Type
42	Reference
4	Source Tissue
8	Specific Activity [micromol/min/mg]
1	Storage Stability
53	Substrates and Products (Substrate)
5	Subunits
51	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]

General Stability

print visible entries

print all entries

General Stability on EC 5.2.1.5 - linoleate isomerase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide Go to General Stability Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

GENERAL STABILITY

ORGANISM

UNIPROT

LITERATURE

the enzyme is very unstable, especially after being solubilized by detergents, increasing glycerol concentration to 30% and salt concentration to 0.5 M improves isomerase stability, resulting in a near full retention of activity in crude extracts for 1 week

Clostridium sporogenes

-

679570

the immobilized enzyme has high operational stability and retains high enzymatic activity after seven cycles of reuse at 37°C. After incubation for 3 h, the immobilized linoleic acid isomerase retains 85% of its initial activity, and then its activity is slowly decreased to 45% within 24 h

Lactobacillus delbrueckii subsp. bulgaricus

-

714488