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Literature summary for 5.2.1.5 extracted from
- Purification and characterization of a membrane-bound linoleic acid isomerase from Clostridium sporogenes (2007), Enzyme Microb. Technol., 40, 831-839.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effects by DTT, ATP, ADP, NAD+, NADH, NADPH or CoA | Clostridium sporogenes |
General Stability
| General Stability | Organism |
|---|---|
| the enzyme is very unstable, especially after being solubilized by detergents, increasing glycerol concentration to 30% and salt concentration to 0.5 M improves isomerase stability, resulting in a near full retention of activity in crude extracts for 1 week | Clostridium sporogenes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 9-cis,12-cis-linoleic acid methyl ester | i.e. methyl linoleate, 31% inhibition at 0.02 mM | Clostridium sporogenes |  |
| 9-cis,12-cis-octadecadien-1-ol | i.e. linoleyl alcohol, 19% inhibition at 0.02 mM | Clostridium sporogenes | |
| gamma-linolenic acid | substrate inhibition at concentrations above 0.015 mM | Clostridium sporogenes | |
| linoleic acid | substrate inhibition at concentrations above 0.02 mM, some linoleic acid derivatives also inhibited the isomerase | Clostridium sporogenes | |
| linolelaidic acid | 81% inhibition at 0.02 mM | Clostridium sporogenes | |
| linolenic acid | substrate inhibition at concentrations above 0.04 mM | Clostridium sporogenes | |
| additional information | no effects by DTT, ATP, ADP, NAD+, NADH, NADPH or CoA | Clostridium sporogenes | |
| oleic acid | 75% inhibition at 0.02 mM | Clostridium sporogenes | |
| palmitoleic acid | 72% inhibition at 0.02 mM | Clostridium sporogenes | |
| stearidonic acid | substrate inhibition at concentrations above 0.120 mM | Clostridium sporogenes | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Clostridium sporogenes | |
| 0.0034 | - |
6-cis,9-cis,12-cis,15-cis-octadecatetraenoic acid | pH 7.5, 22°C | Clostridium sporogenes | |
| 0.0077 | - |
9-cis,12-cis,15-cis-octadecatrienoic acid | pH 7.5, 22°C | Clostridium sporogenes | |
| 0.0119 | - |
9-cis,12-cis-linoleic acid | pH 7.5, 22°C | Clostridium sporogenes | |
| 0.0258 | - |
6-cis,9-cis,12-cis-octadecatrienoic acid | pH 7.5, 22°C | Clostridium sporogenes | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | bound | Clostridium sporogenes | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
4 * 45000, SDS-PAGE | Clostridium sporogenes |
| 190000 | - |
native PAGE | Clostridium sporogenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 9-cis,11-trans-linoleic acid | Clostridium sporogenes | formation only under aerobic conditions | 9-trans,11-trans-linoleic acid | - |
? | |
| 9-cis,12-cis-linoleic acid | Clostridium sporogenes | higher activity under aerobic than under anaerobic conditions | 9-cis,11-trans-linoleic acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium sporogenes | - |
strain ATCC 25762 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 364fold by anion exchange chromatography, chromatofocusing, and gel filtration, method optimization for purification of the very unstable enzyme, overview | Clostridium sporogenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.4 | - |
purified enzyme | Clostridium sporogenes |
Storage Stability
| Storage Stability | Organism |
|---|---|
| the enzyme is very unstable, especially after being solubilized by detergents, increasing glycerol concentration to 30% and salt concentration to 0.5 M improves isomerase stability, resulting in a near full retention of activity in crude extracts for 1 week | Clostridium sporogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 11-cis,14-cis-eicosadienoic acid | - |
Clostridium sporogenes | ? | - |
? | |
| 6-cis,9-cis,12-cis,15-cis-octadecatetraenoic acid | i.e. stearidonic acid | Clostridium sporogenes | ? | - |
? | |
| 6-cis,9-cis,12-cis-octadecatrienoic acid | i.e. alpha-linolenic acid | Clostridium sporogenes | ? | - |
? | |
| 9-cis,11-trans-linoleic acid | formation only under aerobic conditions | Clostridium sporogenes | 9-trans,11-trans-linoleic acid | - |
? | |
| 9-cis,12-cis,15-cis-octadecatrienoic acid | i.e. gamma-linolenic acid | Clostridium sporogenes | ? | - |
? | |
| 9-cis,12-cis-linoleic acid | higher activity under aerobic than under anaerobic conditions | Clostridium sporogenes | 9-cis,11-trans-linoleic acid | - |
? | |
| 9-cis,12-cis-linoleic acid | higher activity under aerobic than under anaerobic conditions | Clostridium sporogenes | 9-cis,11-trans-linoleic acid | conjugated linoleic acid isomer | ? | |
| additional information | substrate specificity, overview, the enzyme is specific for substrates containing cis double bonds at the c9 and c12 positions of C18 polyunsaturated fatty acids, a free carboxyl group is absolutely necessary for isomerization | Clostridium sporogenes | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 45000, SDS-PAGE | Clostridium sporogenes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Linoleic acid isomerase | - |
Clostridium sporogenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Clostridium sporogenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Clostridium sporogenes |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | 9 | - |
Clostridium sporogenes |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no external cofactors are required for catalysis | Clostridium sporogenes |
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