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4.6.1.2: guanylate cyclase

This is an abbreviated version!
For detailed information about guanylate cyclase, go to the full flat file.

Word Map on EC 4.6.1.2

Reaction

GTP
=
3',5'-cyclic GMP
 +
diphosphate

Synonyms

alpha1 sGC, alpha1 soluble guanylyl cyclase, alpha1beta1 guanylyl cyclase, alpha1beta1 sGC, ANF-RGC, ANPRA, ANPRB, atrial natriuretic factor receptor guanylate cyclase, Atrial natriuretic peptide A-type receptor, Atrial natriuretic peptide B-type receptor, atrial natriuretic peptide receptor 2, BERhGC, BR1-GC, brassinosteroid receptor 1, CaRhGC, CNP-sensitive guanyly cyclase, CNP-sensitive guanylyl cyclase, CsGC-YO1, GC-A, GC-A receptor, GC-B, GC-C, GC-D, GC-G, GC1, GC2, GCA, GCC, gcy-18, gcy-23, GCY-35, gcy-8, GTP-pyrophosphate liase cyclizing, GTP-pyrophosphate lyase, guanyl cyclase, guanyl cyclase B, Guanylate cyclase, guanylate cyclase 1, guanylate cyclase 2, Guanylate cyclase 2D, retinal, Guanylate cyclase 2E, Guanylate cyclase 2F, retinal, guanylate cyclase A, guanylate cyclase B, guanylate cyclase C, Guanylate cyclase, olfactory, guanylyl cyclase, guanylyl cyclase A, guanylyl cyclase B, guanylyl cyclase C, guanylyl cyclase C receptor, guanylyl cyclase-A, guanylyl cyclase-A receptor, guanylyl cyclase-C, guanylyl cyclase-D, guanylyl cyclase-G, Gyc-88E, Gyc-89Da, Gyc-89Db, Gyca-99B, Gycb-100B, H-NOX, hSTAR, Intestinal guanylate cyclase, KSGC, L1 H-NOX, L2 H-NOX, membrane bound guanylate cyclase, membrane bound guanylyl cyclase, membrane guanylate cyclase, membrane guanylyl cyclase receptor, membrane-bound GC, membrane-bound guanylate cyclase, membrane-bound guanylyl cyclase, mGc, More, natriuretic peptide-activated guanylate cyclase, nitric oxid sensitive guanylyl cyclase, nitric oxide sensitive guanylyl cyclase, nitric oxide sensitive-guanylyl cyclase, nitric oxide-sensitive guanylate cyclase, nitric-oxide-sensitive guanylyl cyclase, NO receptor, NO receptor soluble guanylyl cyclase, NO sensitive guanylyl cyclase, NO- and haem-independent sGC, NO- and haem-independent soluble guanylate cyclase, NO-GC, NO-GC1, NO-GC2, NO-independent, heme-dependent soluble guanylate cyclase, NO-sensitive GC, NO-sensitive guanylyl cyclase, NO-sGC, NOGC1, NOsGC, NP-GC, NPR A, NPR-A, NPR-B, NPR1, NPR2, ONE-GC, ONE-GC membrane guanylate cyclase, Pac2, Pac3, particulate GC, particulate guanylate cyclase, particulate guanylyl cyclase, PepR1-GC, peptide hormone receptor guanylyl cyclase-C, PGC, photoreceptor guanylyl cyclase, phytosulfokine receptor, PSK receptor, PSKR1, ratGC, receptor guanylyl cyclase, receptor-type guanylyl cyclase, RET-GC1, retGC, RetGC1, RetGC2, retinal guanylate cyclase, retinal guanylyl cyclase, retinal membrane guanylyl cyclase, rGC, rhodopsin-guanylyl cyclase, Rod outer segment membrane guanylate cyclase, rod outer segment membrane guanylate cyclase type 1, ROS-GC, ROS-GC1, ROS-GC2, sGC, sGC alpha2 inhibitory isoform, sGC alpha2 isoform, sGCalpha1, sGCalpha2beta1, sGCbeta1, sGCbeta3, soluble GC, soluble guanylate cyclase, soluble guanylate cyclase alpha1, soluble guanylate cyclase alpha2, soluble guanylyl cyclase, SONO, STA, STA receptor, Tar4 protein

ECTree

     4 Lyases
         4.6 Phosphorus-oxygen lyases
             4.6.1 Phosphorus-oxygen lyases (only sub-subclass identified to date)
                4.6.1.2 guanylate cyclase

Crystallization

Crystallization on EC 4.6.1.2 - guanylate cyclase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
H-NOX domain of Tar4H, residues form hydrogen bonds to the heme group, tyrosine at position 140 is critical in discriminating between NO and O2
-
to 1.77 A and 2.5 A resolution, N-terminal heme domain and C-terminal catalytic domain
-
crystal structure of the ligand-bound adenylyl cyclase (resulting from the point mutations E497K/C566D) domain at 2.25 A reveals the mechanistic basis for the change from cGMP to cAMP production
isolated dimeric and inactive catalytic domain, X-ray diffraction structure determination and analysis at 2.55 A resolution
homology modeling of the catalytic domain in inactive or active conformations based on PDB entries 2WZ1, 3ET6, 1CJU and molecular dynamics simulations reveals presence of potential high-affinity binding site on the active structure. The site is located between the pseudo-symmetric and the catalytic site just over the loop beta2-beta3 and does not overlap with the forskolin binding site on adenylate cyclases
sitting drop vapor diffusion method, using 0.05 M KH2PO4 and 20% (w/v) PEG 8000
sitting drop vapor diffusion method, using 20% (w/v) PEG3350, 50 mM HEPES (pH 7.0), and 1% (w/v) tryptone
sitting drop vapor diffusion method, using 1.4-1.6 M ammonium sulfate, 50 mM sodium cacodylate (pH 5.5-6.5) and 15 mM magnesium acetate tetrahydrate
sitting drop vapor diffusion method, using 0.1 M Bis-Tris pH 7.0, and 0.7 M ammonium sulfate