4.1.3.3: N-acetylneuraminate lyase
This is an abbreviated version!
For detailed information about N-acetylneuraminate lyase, go to the full flat file.
Word Map on EC 4.1.3.3
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4.1.3.3
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mannac
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2-epimerase
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n-acetylmannosamine
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aldolases
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n-glycolylneuraminic
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cmp-sialic
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dihydrodipicolinate
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3.2.1.18
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biotechnology
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neu5gc
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dhdps
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synthesis
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n-acylneuraminate
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analysis
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industry
- 4.1.3.3
- mannac
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2-epimerase
- n-acetylmannosamine
- aldolases
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n-glycolylneuraminic
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cmp-sialic
- dihydrodipicolinate
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3.2.1.18
- biotechnology
- neu5gc
- dhdps
- synthesis
- n-acylneuraminate
- analysis
- industry
Reaction
Synonyms
Acetylneuraminate pyruvate-lyase, class I NAL, EcNanA, Lyase, acetylneuraminate, N-acetyl-d-neuraminic acid aldolase, N-acetyl-D-neuraminic acid lyase, N-Acetylneuraminate aldolase, N-Acetylneuraminate lyase, N-acetylneuraminate pyruvate lyase, N-Acetylneuraminate pyruvate-lyase, N-Acetylneuraminic acid aldolase, N-Acetylneuraminic acid lyase, N-Acetylneuraminic aldolase, N-Acetylneuraminic lyase, NAL, NALase, NanA, NANA lyase, Neu5Ac aldolase, Neu5Ac lyase, NeuAc aldolase, NeuAc lyase, Neuraminate aldolase, Neuraminic acid aldolase, Neuraminic aldolase, NPL, PmNanA, sialate aldolase, Sialate lyase, sialate-pyruvate lyase, Sialic acid aldolase, Sialic aldolase
ECTree
4.1.3.3 N-acetylneuraminate lyaseAdvanced search results
results (
results (Temperature Stability
Temperature Stability on EC 4.1.3.3 - N-acetylneuraminate lyase
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TEMPERATURE STABILITY 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
20 - 50
purified recombinant His-tagged enzyme, pH 7.0, completely stable over 140-160 min
37
37 - 70
the enzyme remains stable after incubation for 150 min at 37°C. When incubated at 70°C, the half-life for enzymatic activity is 30 min and that for a complete loss of activity is 1 h
40
when the enzyme is incubated at 40°C, pH 8.5 for 48 h, in N-acetylneuraminate synthesis direction, the enzyme keeps 100% activity for the first 24 h but remains 60% of its activity in 48h. 75% and 20% of N-acetylneuraminate cleavage activity remain in 24 h and 48 h, respectively
50
60
70
80
additional information
37
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in 50 mM Tris/HCl, pH 8, 25 mM MgCl2, 1 mM PMSF stable for at least 24 h
50
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cross-linked enzyme aggregates, stable at pH 9.0 for 6 hours as the soluble enzyme
60
purified recombinant His-tagged enzyme, pH 7.0, 120 min, 60% activity remaining
70
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50% loss of activity after 10 min, in presence of 80 mM pyruvate full activity retained after 1 h
70
purified recombinant His-tagged enzyme, pH 7.0, inactivation within 60 min
80
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77% loss of activity after 10 min, in presence of 5 mM pyruvate 8% loss of activity after 10 min
80
purified recombinant His-tagged enzyme, pH 7.0, inactivation within 10 min
additional information
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thermostability of enzyme produced by recombinant Serratia liquefaciens is higher by about 10°C than thermostability of enzyme produced by recombinant E. coli
