4.1.3.3: N-acetylneuraminate lyase
This is an abbreviated version!
For detailed information about N-acetylneuraminate lyase, go to the full flat file.
Word Map on EC 4.1.3.3
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4.1.3.3
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mannac
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2-epimerase
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n-acetylmannosamine
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aldolases
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n-glycolylneuraminic
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cmp-sialic
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dihydrodipicolinate
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3.2.1.18
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biotechnology
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neu5gc
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dhdps
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synthesis
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n-acylneuraminate
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analysis
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industry
- 4.1.3.3
- mannac
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2-epimerase
- n-acetylmannosamine
- aldolases
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n-glycolylneuraminic
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cmp-sialic
- dihydrodipicolinate
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3.2.1.18
- biotechnology
- neu5gc
- dhdps
- synthesis
- n-acylneuraminate
- analysis
- industry
Reaction
Synonyms
Acetylneuraminate pyruvate-lyase, class I NAL, EcNanA, Lyase, acetylneuraminate, N-acetyl-d-neuraminic acid aldolase, N-acetyl-D-neuraminic acid lyase, N-Acetylneuraminate aldolase, N-Acetylneuraminate lyase, N-acetylneuraminate pyruvate lyase, N-Acetylneuraminate pyruvate-lyase, N-Acetylneuraminic acid aldolase, N-Acetylneuraminic acid lyase, N-Acetylneuraminic aldolase, N-Acetylneuraminic lyase, NAL, NALase, NanA, NANA lyase, Neu5Ac aldolase, Neu5Ac lyase, NeuAc aldolase, NeuAc lyase, Neuraminate aldolase, Neuraminic acid aldolase, Neuraminic aldolase, NPL, PmNanA, sialate aldolase, Sialate lyase, sialate-pyruvate lyase, Sialic acid aldolase, Sialic aldolase
ECTree
4.1.3.3 N-acetylneuraminate lyaseAdvanced search results
results (
results (KM Value
KM Value on EC 4.1.3.3 - N-acetylneuraminate lyase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.39 - 11
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide
0.35 - 10.6
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
additional information
additional information
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with substrate N-acetylneuraminic acid increasing Km with increasing pH
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0.39
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide
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mutant enzyme E192N
11
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide
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wild-type enzyme
0.35
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
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pH 7.5, 25°C, mutant enzyme E192V
0.39
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
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pH 7.5, 25°C, mutant enzyme E192N
0.5
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
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pH 7.5, 25°C, mutant enzyme E192S
2.54
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
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pH 7.5, 25°C, mutant enzyme S208G
5.7
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
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pH 7.5, 25°C, mutant enzyme D191P
10.6
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
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pH 7.5, 25°C, wild-type enzyme
0.2
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
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E192M mutant protein, pH 7.5, 25°C
0.2
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
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E192P mutant protein, pH 7.5, 25°C
0.3
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
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E192F mutant protein, pH 7.5, 25°C
0.4
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
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E192N mutant protein, pH 7.7.5, 25°C
0.4
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
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E192V mutant protein, pH 7.5, 25°C
0.5
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
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E192H mutant protein, pH 7.5, 25°C
0.6
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
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E192S mutant protein, pH 7.5, 25°C
0.7
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
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E192Q mutant protein, pH 7.5, 25°C
1.1
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
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E192D mutant protein, pH 7.5, 25°C
11
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
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wild type (E192), pH 7.5, 25°C
3.5
D-3-deoxy-manno-2-octulosonic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I
6.3
D-3-deoxy-manno-2-octulosonic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
11.4
D-3-deoxy-manno-2-octulosonic acid
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pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
12.3
D-3-deoxy-manno-2-octulosonic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L
14.5
D-3-deoxy-manno-2-octulosonic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I
2.4
L-3-deoxy-manno-2-octulosonic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
2.7
L-3-deoxy-manno-2-octulosonic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I
2.8
L-3-deoxy-manno-2-octulosonic acid
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pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
9.2
L-3-deoxy-manno-2-octulosonic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I
14.1
L-3-deoxy-manno-2-octulosonic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L
131.7
N-acetyl-D-mannosamine
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recombinant enzyme, pH and temperature not specified in the publication
149
N-acetyl-D-mannosamine
recombinant His-taggged enzyme, pH 7.0, 37°C
160
N-acetyl-D-mannosamine
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soluble enzyme, pH not specified in the publication, temperature not specified in the publication
245
N-acetyl-D-mannosamine
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cross-linked enzyme aggregates, pH 7.5, 37°C
333
N-acetyl-D-mannosamine
wild type enzyme, at pH 7.0 and 37°C
915
N-acetyl-D-mannosamine
mutant enzyme P193Y, at pH 7.0 and 37°C
3200
N-acetyl-D-mannosamine
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
3.9
N-acetyl-D-neuraminic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I
10.1
N-acetyl-D-neuraminic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
12.1
N-acetyl-D-neuraminic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I
15.3
N-acetyl-D-neuraminic acid
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pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
3 - 3.5
N-acetyl-L-neuraminic acid
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pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
36.2
N-acetyl-L-neuraminic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
127
N-acetyl-L-neuraminic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I
369.4
N-acetyl-L-neuraminic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I
0.013
N-acetylneuraminate
purified recombinant mutant K165C, pH 7.4, 30°C
0.015
N-acetylneuraminate
purified recombinant mutant K165-gamma-thialysine, pH 6.8, 30°C
0.023
N-acetylneuraminate
purified recombinant mutant K165-gamma-thialysine, pH 7.4, 30°C
0.036
N-acetylneuraminate
purified recombinant wild-type enzyme, pH 7.4, 30°C
0.04
N-acetylneuraminate
purified recombinant wild-type enzyme, pH 6.8, 30°C
0.27
N-acetylneuraminate
mutant enzyme P193Y, at pH 7.0 and 37°C
1.1
N-acetylneuraminate
wild type enzyme, at pH 7.0 and 37°C
1.2
N-acetylneuraminate
mutant enzyme G211S, at pH 7.0 and 37°C
2
N-acetylneuraminate
recombinant His-taggged enzyme, pH 7.0, 37°C
2
N-acetylneuraminate
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
2.51
N-acetylneuraminate
mutant enzyme L171N/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
3.2
N-acetylneuraminate
mutant enzyme L199N/I229D, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
3.2
N-acetylneuraminate
at 30°C, pH not specified in the publication
3.4
N-acetylneuraminate
mutant enzyme I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
3.42
N-acetylneuraminate
mutant enzyme L171R, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
3.93
N-acetylneuraminate
mutant enzyme I229R, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
4
N-acetylneuraminate
mutant enzyme L199N/I229R, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
4
N-acetylneuraminate
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recombinant enzyme, pH and temperature not specified in the publication
4.1
N-acetylneuraminate
wild type enzyme, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
4.3
N-acetylneuraminate
mutant enzyme L199N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
4.7
N-acetylneuraminate
mutant enzyme L199N/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
5
N-acetylneuraminate
mutant enzyme I229D, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
6.1
N-acetylneuraminate
mutant enzyme L199R/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
2
N-acetylneuraminic acid
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pH 7.2, 50 mM phosphate buffer, wild-type
3.2
N-acetylneuraminic acid
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pH 7.2, 250 mM phosphate buffer, wild-type
19.9
pyruvate
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soluble enzyme, pH not specified in the publication, temperature not specified in the publication
35.1
pyruvate
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recombinant enzyme, pH and temperature not specified in the publication
