4.1.3.3: N-acetylneuraminate lyase
This is an abbreviated version!
For detailed information about N-acetylneuraminate lyase, go to the full flat file.
Word Map on EC 4.1.3.3
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4.1.3.3
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mannac
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2-epimerase
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n-acetylmannosamine
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aldolases
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n-glycolylneuraminic
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cmp-sialic
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dihydrodipicolinate
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3.2.1.18
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biotechnology
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neu5gc
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dhdps
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synthesis
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n-acylneuraminate
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analysis
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industry
- 4.1.3.3
- mannac
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2-epimerase
- n-acetylmannosamine
- aldolases
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n-glycolylneuraminic
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cmp-sialic
- dihydrodipicolinate
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3.2.1.18
- biotechnology
- neu5gc
- dhdps
- synthesis
- n-acylneuraminate
- analysis
- industry
Reaction
Synonyms
Acetylneuraminate pyruvate-lyase, class I NAL, EcNanA, Lyase, acetylneuraminate, N-acetyl-d-neuraminic acid aldolase, N-acetyl-D-neuraminic acid lyase, N-Acetylneuraminate aldolase, N-Acetylneuraminate lyase, N-acetylneuraminate pyruvate lyase, N-Acetylneuraminate pyruvate-lyase, N-Acetylneuraminic acid aldolase, N-Acetylneuraminic acid lyase, N-Acetylneuraminic aldolase, N-Acetylneuraminic lyase, NAL, NALase, NanA, NANA lyase, Neu5Ac aldolase, Neu5Ac lyase, NeuAc aldolase, NeuAc lyase, Neuraminate aldolase, Neuraminic acid aldolase, Neuraminic aldolase, NPL, PmNanA, sialate aldolase, Sialate lyase, sialate-pyruvate lyase, Sialic acid aldolase, Sialic aldolase
ECTree
4.1.3.3 N-acetylneuraminate lyaseAdvanced search results
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results (Turnover Number
Turnover Number on EC 4.1.3.3 - N-acetylneuraminate lyase
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TURNOVER NUMBER [1/s] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.057 - 10.83
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide
0.44 - 2.18
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
0.057 - 0.65
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide
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mutant enzyme E192N
1.23
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide
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wild-type enzyme
10.83
(2R,3R,4R)-6-[(aminooxy)carbonyl]-3,4,6-trihydroxy-N-propyltetrahydro-2H-pyran-2-carboxamide
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mutant enzyme E192N
0.44
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
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pH 7.5, 25°C, mutant enzyme S208G
0.91
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
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pH 7.5, 25°C, mutant enzyme D191P
1.22
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
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pH 7.5, 25°C, wild-type enzyme
1.58
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
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pH 7.5, 25°C, mutant enzyme E192V
1.85
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
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pH 7.5, 25°C, mutant enzyme E192S
2.18
(2S,4S,5R,6R)-5-(acetylamino)-6-[(dipropylamino)carbonyl]-2,4-dihydroxytetrahydro-pyran-2-carboxylic acid
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pH 7.5, 25°C, mutant enzyme E192N
0.1
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
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E192D mutant protein, pH 7.5, 25°C
0.43
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
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E192S mutant protein, pH 7.5, 25°C
0.63
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
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E192H mutant protein, pH 7.5, 25°C
0.82
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
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E192P mutant protein, pH 7.5, 25°C
1.17
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
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E192M mutant protein, pH 7.5, 25°C
1.22
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
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wild type (E192), pH 7.5, 25°C
1.47
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
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E192F mutant protein, pH 7.5, 25°C
1.58
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
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E192V mutant protein, pH 7.5, 25°C
2.17
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
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E192N mutant protein, pH 7.7.5, 25°C
2.83
(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
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E192Q mutant protein, pH 7.5, 25°C
1.5
D-3-deoxy-manno-2-octulosonic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L
2.8
D-3-deoxy-manno-2-octulosonic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I
3.2
D-3-deoxy-manno-2-octulosonic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I
5.1
D-3-deoxy-manno-2-octulosonic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
8.9
D-3-deoxy-manno-2-octulosonic acid
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pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
1.1
L-3-deoxy-manno-2-octulosonic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L
1.9
L-3-deoxy-manno-2-octulosonic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I
2.5
L-3-deoxy-manno-2-octulosonic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I
4.2
L-3-deoxy-manno-2-octulosonic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
10.4
L-3-deoxy-manno-2-octulosonic acid
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pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
2.4
N-acetyl-D-mannosamine
mutant enzyme P193Y, at pH 7.0 and 37°C
4.5
N-acetyl-D-mannosamine
recombinant His-taggged enzyme, pH 7.0, 37°C
5.4
N-acetyl-D-mannosamine
wild type enzyme, at pH 7.0 and 37°C
15.3
N-acetyl-D-mannosamine
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
1.5
N-acetyl-D-neuraminic acid
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pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
1.7
N-acetyl-D-neuraminic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
2.2
N-acetyl-D-neuraminic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I
9.7
N-acetyl-D-neuraminic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I
0.0045
N-acetyl-L-neuraminic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I
0.0068
N-acetyl-L-neuraminic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L/V251I/V265I
0.02
N-acetyl-L-neuraminic acid
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pH 7.5, 25°C, mutant enzyme E60A/Y98H/F115L/N153Y/D150G/V251I/V265I/Y281C/N153Y
0.02
N-acetyl-L-neuraminic acid
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pH 7.5, 25°C, mutant enzyme Y98H/F115L/N153Y/V251I/V265I/Y281C
0.013
N-acetylneuraminate
purified recombinant mutant K165C, pH 7.4, 30°C
0.015
N-acetylneuraminate
purified recombinant mutant K165-gamma-thialysine, pH 6.8, 30°C
0.023
N-acetylneuraminate
purified recombinant mutant K165-gamma-thialysine, pH 7.4, 30°C
0.032
N-acetylneuraminate
mutant enzyme G211S, at pH 7.0 and 37°C
0.036
N-acetylneuraminate
purified recombinant wild-type enzyme, pH 7.4, 30°C
0.04
N-acetylneuraminate
purified recombinant wild-type enzyme, pH 6.8, 30°C
0.13
N-acetylneuraminate
mutant enzyme P193Y, at pH 7.0 and 37°C
0.29
N-acetylneuraminate
mutant enzyme L199N/I229D, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
0.47
N-acetylneuraminate
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C
0.664
N-acetylneuraminate
mutant enzyme L199N/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
1.9
N-acetylneuraminate
wild type enzyme, at pH 7.0 and 37°C
3.15
N-acetylneuraminate
mutant enzyme L171N/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
4
N-acetylneuraminate
recombinant His-taggged enzyme, pH 7.0, 37°C
4.69
N-acetylneuraminate
mutant enzyme I229D, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
5.49
N-acetylneuraminate
mutant enzyme L171R, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
6.75
N-acetylneuraminate
wild type enzyme, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
7.4
N-acetylneuraminate
mutant enzyme I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
7.72
N-acetylneuraminate
mutant enzyme I229R, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
7.94
N-acetylneuraminate
mutant enzyme L199N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
8.9
N-acetylneuraminate
mutant enzyme L199N/I229R, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
11.87
N-acetylneuraminate
mutant enzyme L199R/I229N, in sodium phosphate buffer (100 mM, pH 7.6), at 30°C
22.1
N-acetylneuraminate
at 30°C, pH not specified in the publication
