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3.6.4.7: peroxisome-assembly ATPase

This is an abbreviated version!
For detailed information about peroxisome-assembly ATPase, go to the full flat file.

Word Map on EC 3.6.4.7

Reaction

ATP
+
H2O
=
ADP
 +
phosphate

Synonyms

AAA protein, AAA+-ATPase, AAA+-type II ATPase, AAA-ATPase, AAA-complex, AFG1, APEM10, AtLon2, ATP-dependent lon protease, ATPase associated with diverse cellular activities, ATPase associated with various cellular activities, Cdc48, Cdc48p, HpPln, lon, lon protease, LON protease 2, lon2, LONP2, Mm-p, Nc-p, NSF, NSF-like ATPase, p97/VCP, PAF-2, PAS1, PAS1p, PAS8, peroxin, peroxin ATPase complex, peroxisomal AAA ATPase complex, peroxisomal AAA complex, peroxisomal Lon protease, peroxisomal Lon-protease, peroxisomal Pex1/Pex6 ATPase complex, peroxisomal protease LON2, peroxisomal receptor export complex, peroxisome assembly factor-2, peroxisome-assembly ATPase, peroxisome-associated ATPase, Pex1, PEX1/6, Pex1/6 AAA+ complex, Pex1/6 type II AAA+ complex, Pex1/6p-complex, Pex1/Pex6, Pex1/Pex6 complex, Pex1p, Pex1p-Pex6p complex, Pex1p-Pex6p-complex, Pex1p/Pex6p, PEX6, Pex6 AAA ATPase, Pex6p, Pln, REM, SEC18p, TBP1, valosin-containing protein, VCP, Yl-p, Zm-p

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.4 Acting on acid anhydrides to facilitate cellular and subcellular movement
                3.6.4.7 peroxisome-assembly ATPase

Purification

Purification on EC 3.6.4.7 - peroxisome-assembly ATPase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
co-purification of recombinant Strep-tagged Pex1 and His6-tagged Pex6 from yeast cells by sequential affinity chromatography on Ni-NTA and streptavidin agarose resins, followed by gel filtration
copurification of recombinant FLAG-tagged Pex1 and His-tagged Pex6 from Escherichia coli by tandem-affinity chromatography using Ni-NTA agarose and alpha-FLAG affinity resin, followed by gel filtration
Ni-NTA column chromatography, anti-FLAG M2 affinity resin column chromatography, and Superose 6 gel filtration
-
Ni-NTA column chromatography, GSH-agarose column chromatography and Superose 6 gel filtration
-
partially, peroxisomes isolated from rat liver by usual cell fractionation are further purified by immunoisolation using a specific antibody raised against a peroxisomal membrane protein, PMP70
recombinant MBP-fusion enzyme from Escherichia coli strain Rosetta 2 to homogeneity
-
recombinant N-terminally His6-tagged and C-terminally GST-tagged Pex1p/His-Pex6p-complex from Escherichia coli strain Tuner(DE3) by nickel affinity chromatography and gel filtration
using glutathione Sepharose
-