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3.6.4.7: peroxisome-assembly ATPase

This is an abbreviated version!
For detailed information about peroxisome-assembly ATPase, go to the full flat file.

Word Map on EC 3.6.4.7

Reaction

ATP
+
H2O
=
ADP
 +
phosphate

Synonyms

AAA protein, AAA+-ATPase, AAA+-type II ATPase, AAA-ATPase, AAA-complex, AFG1, APEM10, AtLon2, ATP-dependent lon protease, ATPase associated with diverse cellular activities, ATPase associated with various cellular activities, Cdc48, Cdc48p, HpPln, lon, lon protease, LON protease 2, lon2, LONP2, Mm-p, Nc-p, NSF, NSF-like ATPase, p97/VCP, PAF-2, PAS1, PAS1p, PAS8, peroxin, peroxin ATPase complex, peroxisomal AAA ATPase complex, peroxisomal AAA complex, peroxisomal Lon protease, peroxisomal Lon-protease, peroxisomal Pex1/Pex6 ATPase complex, peroxisomal protease LON2, peroxisomal receptor export complex, peroxisome assembly factor-2, peroxisome-assembly ATPase, peroxisome-associated ATPase, Pex1, PEX1/6, Pex1/6 AAA+ complex, Pex1/6 type II AAA+ complex, Pex1/6p-complex, Pex1/Pex6, Pex1/Pex6 complex, Pex1p, Pex1p-Pex6p complex, Pex1p-Pex6p-complex, Pex1p/Pex6p, PEX6, Pex6 AAA ATPase, Pex6p, Pln, REM, SEC18p, TBP1, valosin-containing protein, VCP, Yl-p, Zm-p

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.4 Acting on acid anhydrides to facilitate cellular and subcellular movement
                3.6.4.7 peroxisome-assembly ATPase

Cloned

Cloned on EC 3.6.4.7 - peroxisome-assembly ATPase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a GST fusion protein containing the PEX1 gene encoding residues 3-180, the N-terminal domain, in pGEX-4T3-PRESAT is constructed
-
AFG1 gene sequenced
-
AtLon2 expression profile
expressed in Escherichia coli BL21
-
expressed in Escherichia coli Tuner (DE3) cells
-
gene At5g47040, DNA and amino acid sequence determination and analysis, isozyme LON2 is encoded by gene aberrant peroxisome morphology 10, apem10
gene Lon1, phylogenetic tree
gene Lonp2, DNA and amino acid sequence determination and analysis, genetic structure
gene Lonp2, phylogenetic tree
gene NCU08303, phylogenetic tree
gene PEX1, functional expression of FLAG-tagged Pex1 in Saccharomyces cerevisiae, FLAG-tagged Pex1 and His-tagged Pex6 coexpression in Escherichia coli
gene PEX6, functional expression of FLAG-tagged Pex6 in Saccharomyces cerevisiae, FLAG-tagged Pex1 and His-tagged Pex6 coexpression in Escherichia coli
gene PLN, phylogenetic tree, recombinant expression of HpPln, a GFP-PLN hybrid gene under control of the PLN promoter, constructed for the subcellular localization study, coexpression of te peroxisomal marker GFP-SKL in Hansenula polymorpha
gene pln, recombinant expression of GFP-tagged enzyme in GFP-SKL protoplasts, recombinant expression of Pln protein fused to maltose-binding protein (MBP) at the N-terminus and a His6-tag at the C-terminus, with a tobacco etch virus (TEV) cleavage site between MBP and Pln, in Escherichia coli strain Rosetta 2
-
gene YALI0F23595g, phylogenetic tree
genes PEX1 and PEX6 cloned by functional complementation of corresponding peroxisome-deficient pex mutants
-
organization and cloning of a recombinant Pex1p/Pex6p-complex, recombinant expression of the N-terminally His6-tagged and C-terminally GST-tagged Pex1p/His-Pex6p-complex in Escherichia coli strain Tuner(DE3), ATP-dependence of the Pex1p/Pex6p complex formation
overexpression of the pex1p/pex6p complex in Escherichia coli or yeast, the complex assembles in the presence of a nucleotide
PCR amplification, full-length human PAF-2 cDNA cloned
-
PCR-based cDNA cloning of human PAF-2 to identify mutations in the PAF-2 gene of patients using a mammalian expression vector is under way
-
peroxisome assembly factor-2 cloned from rat cDNA
-
PEX1 and PEX6 genes cloned
PEX1 and PEX6 genes cloned and sequenced
-
recombinant coexpression of Pex1 and Pex6 in yeast cells with an N-terminal SBP tag on Pex1 and an N-terminal His14 tag on Pex6