Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.4.7 extracted from

  • Grimm, I.; Saffian, D.; Girzalsky, W.; Erdmann, R.
    Nucleotide-dependent assembly of the peroxisomal receptor export complex (2016), Sci. Rep., 6, 19838 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.4.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Tuner (DE3) cells Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Saccharomyces cerevisiae 5829
-
peroxisomal membrane
-
 Saccharomyces cerevisiae 5778
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
700000
-
 in the presence of ATP, Pex6p and Pex1p both peaked at a size of approximately 700000 Da, gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Saccharomyces cerevisiae
-
 ADP + phosphate
-
 ?
additional information Saccharomyces cerevisiae the Pex1p/Pex6p enzyme complex is able to bind efficiently to Pex15p in vivo. Pex6p mediates binding to the cytosolic part of Pex15p via a direct interaction ?
-
-

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, GSH-agarose column chromatography and Superose 6 gel filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Saccharomyces cerevisiae ADP + phosphate
-
 ?
additional information the Pex1p/Pex6p enzyme complex is able to bind efficiently to Pex15p in vivo. Pex6p mediates binding to the cytosolic part of Pex15p via a direct interaction Saccharomyces cerevisiae ?
-
-

Subunits

Subunits Comment Organism
heterohexamer
-
 Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
AAA-ATPase
-
 Saccharomyces cerevisiae
AAA-complex
-
 Saccharomyces cerevisiae
peroxisomal receptor export complex
-
 Saccharomyces cerevisiae
Pex1/6p-complex
-
 Saccharomyces cerevisiae
Pex1p
-
 Saccharomyces cerevisiae
Pex6p
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
metabolism the two AAA-ATPases Pex1p and Pex6p are required for biogenesis of peroxisomes. At the peroxisomal membrane, the enzyme complex is responsible for the release of the import receptor Pex5p at the end of the matrix protein import cycle Saccharomyces cerevisiae