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Search Purification (Commentary)

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Results 1 - 9 of 9
EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 3.6.4.7-
Display the word mapDisplay the reaction diagram Show all sequences 3.6.4.7co-purification of recombinant Strep-tagged Pex1 and His6-tagged Pex6 from yeast cells by sequential affinity chromatography on Ni-NTA and streptavidin agarose resins, followed by gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 3.6.4.7copurification of recombinant FLAG-tagged Pex1 and His-tagged Pex6 from Escherichia coli by tandem-affinity chromatography using Ni-NTA agarose and alpha-FLAG affinity resin, followed by gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 3.6.4.7Ni-NTA column chromatography, anti-FLAG M2 affinity resin column chromatography, and Superose 6 gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 3.6.4.7Ni-NTA column chromatography, GSH-agarose column chromatography and Superose 6 gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 3.6.4.7partially, peroxisomes isolated from rat liver by usual cell fractionation are further purified by immunoisolation using a specific antibody raised against a peroxisomal membrane protein, PMP70
Display the word mapDisplay the reaction diagram Show all sequences 3.6.4.7recombinant MBP-fusion enzyme from Escherichia coli strain Rosetta 2 to homogeneity
Display the word mapDisplay the reaction diagram Show all sequences 3.6.4.7recombinant N-terminally His6-tagged and C-terminally GST-tagged Pex1p/His-Pex6p-complex from Escherichia coli strain Tuner(DE3) by nickel affinity chromatography and gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 3.6.4.7using glutathione Sepharose
Results 1 - 9 of 9