EC Number |
---|
3.6.4.7 | - |
3.6.4.7 | co-purification of recombinant Strep-tagged Pex1 and His6-tagged Pex6 from yeast cells by sequential affinity chromatography on Ni-NTA and streptavidin agarose resins, followed by gel filtration |
3.6.4.7 | copurification of recombinant FLAG-tagged Pex1 and His-tagged Pex6 from Escherichia coli by tandem-affinity chromatography using Ni-NTA agarose and alpha-FLAG affinity resin, followed by gel filtration |
3.6.4.7 | Ni-NTA column chromatography, anti-FLAG M2 affinity resin column chromatography, and Superose 6 gel filtration |
3.6.4.7 | Ni-NTA column chromatography, GSH-agarose column chromatography and Superose 6 gel filtration |
3.6.4.7 | partially, peroxisomes isolated from rat liver by usual cell fractionation are further purified by immunoisolation using a specific antibody raised against a peroxisomal membrane protein, PMP70 |
3.6.4.7 | recombinant MBP-fusion enzyme from Escherichia coli strain Rosetta 2 to homogeneity |
3.6.4.7 | recombinant N-terminally His6-tagged and C-terminally GST-tagged Pex1p/His-Pex6p-complex from Escherichia coli strain Tuner(DE3) by nickel affinity chromatography and gel filtration |
3.6.4.7 | using glutathione Sepharose |