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Literature summary for 3.6.4.7 extracted from

  • Gardner, B.M.; Castanzo, D.T.; Chowdhury, S.; Stjepanovic, G.; Stefely, M.S.; Hurley, J.H.; Lander, G.C.; Martin, A.
    The peroxisomal AAA-ATPase Pex1/Pex6 unfolds substrates by processive threading (2018), Nat. Commun., 9, 135 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.4.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
T246A the mutation of the Pex6 peptide decreases the apparent affinity of Pex1/Pex6 for Pex15 from 0.0006 to 0.002 mM Saccharomyces cerevisiae
V245A the mutation of the Pex6 peptide decreases the apparent affinity of Pex1/Pex6 for Pex15 from 0.0006 to 0.002 mM Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Pex15 Pex15 inhibits the ATPase activity of Pex1/Pex6 in a pore-loop-dependent manner because its cytosolic portion is recognized as a substrate Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
 Saccharomyces cerevisiae 5777
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Saccharomyces cerevisiae
-
 ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, anti-FLAG M2 affinity resin column chromatography, and Superose 6 gel filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Saccharomyces cerevisiae ADP + phosphate
-
 ?
additional information the protein translocase unfolds Pex15 in a pore-loop-dependent and ATP-hydrolysis-dependent manner. Pex15 binds the N-terminal domains of enzyme Pex6, before its C-terminal disordered region engages with the pore loops of the motor, which then processively threads Pex15 through the central pore. Furthermore, Pex15 directly binds the cargo receptor Pex5, linking Pex1/Pex6 to other components of the peroxisomal import machinery Saccharomyces cerevisiae ?
-
-

Subunits

Subunits Comment Organism
heterohexamer Pex1 and Pex6 form a single, heterohexameric type-2 AAA-ATPase motor Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
AAA-ATPase
-
 Saccharomyces cerevisiae
Pex1/Pex6
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
physiological function the enzyme complex Pex1/Pex6 plays a role in mechanical unfolding of peroxins or their extraction from the peroxisomal membrane during matrix-protein import Saccharomyces cerevisiae