3.5.1.25: N-acetylglucosamine-6-phosphate deacetylase This is an abbreviated version!go to the full flat file .
Reaction
N-acetyl-D-glucosamine 6-phosphate H2O D-glucosamine 6-phosphate acetate
Synonyms 2-acetamido-2-deoxy-D-glucose-6-phosphate amidohydrolase, acetylaminodeoxyglucosephosphate acetylhydrolase, acetylglucosamine phosphate deacetylase, CaNAG2/Dac1, deacetylase, acetylglucosaminephosphate, EC 3.5.1.80, GlcNAc 6-P deacetylase, GlcNAc-6-phosphate deacetylase, GlnNAc6P deacetylase, Lmo0956, Lmo0956 protein, Lmo2108, Lmo2108 protein, MMNagA, MSNagA, N-acetyl-D-glucosamine-6-phosphate deacetylase, N-acetylglucosamine 6-phosphate deacetylase, N-acetylglucosamine-6-P deacetylase, N-acetylglucosamine-6-phosphate de-N-acetylase, N-acetylglucosamine-6-phosphate deacetylase, NAG2, NagA, NAGPase
ECTree
Inhibitors
Inhibitors on EC 3.5.1.25 - N-acetylglucosamine-6-phosphate deacetylase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5,5'-dithio-bis(2-nitrobenzoate)
-
-
fructose 1,6-diphosphate
-
72% inhibition at 0.2 mM and 90% inhibition at 0.7 mM
methyl phosphonamidates inhibitor 1
-
tetrahedral reaction intermediate analogues
N-acetyl-D-glucosamine 6-phosphate
-
substrate inhibition at high concentrations
N-methylhydroxyphosphinyl-D-glucosamine 6-phosphate
-
NEM
-
1 mM, complete inhibition
PCMB
-
0.1 mM, complete inhibition
UDP-N-acetylglucosamine
-
10 mM, 35% inhibition
UTP
-
7.5 mM, 21% inhibition
1,10-phenanthroline
-
1,10-phenanthroline
results in unfolding and loss of catalytic activity at 10 mM
acetate
-
no inhibition
Cu2+
-
-
Cu2+
-
1 mM, complete inhibition
EDTA
-
-
EDTA
-
some reversal by MgCl2, CoCl2 restores activity and stimulates above the control value
EDTA
results in unfolding and loss of catalytic activity at 10 mM
EDTA
-
1 mM, 25% inhibition
fructose-6-phosphate
-
40 mM, 75% inhibition
fructose-6-phosphate
-
20% inhibition at 0.3 mM, 30% inhibition at 0.9 mM
glucosamine 6-phosphate
-
competitive
glucosamine 6-phosphate
-
-
glucosamine 6-phosphate
-
mixed type inhibition
glucosamine 6-phosphate
-
87% inhibition at 0.4 mM, 95% inhibition at 1.2 mM
glucosamine 6-phosphate
-
-
glucose 6-phosphate
-
55% inhibition at 0.35 mM, 80% inhibition at 1.1 mM
additional information
enzyme inactivation by metal-sequestering agents and subsequent reactivation by the addition of several divalent cations
-
additional information
-
enzyme inactivation by metal-sequestering agents and subsequent reactivation by the addition of several divalent cations
-
additional information
after treatment with 10 mM 1,10-phenanthroline or 10 mM EDTA, resulting in unfolding and loss of catalytic activity, the activity cannot be restored by addition of ZnCl2 to the metal-free enzyme, indicating that the metal ions are tightly coordinated in the active site and have an essential structural role in mycobacterial NagA enzymes
-
additional information
-
after treatment with 10 mM 1,10-phenanthroline or 10 mM EDTA, resulting in unfolding and loss of catalytic activity, the activity cannot be restored by addition of ZnCl2 to the metal-free enzyme, indicating that the metal ions are tightly coordinated in the active site and have an essential structural role in mycobacterial NagA enzymes
-
3.5.1.25 N-acetylglucosamine-6-phosphate deacetylase
results (
results (
top
