Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, complete inhibition | Vibrio cholerae serotype O1 | |
EDTA | 1 mM, 25% inhibition | Vibrio cholerae serotype O1 | |
NEM | 1 mM, complete inhibition | Vibrio cholerae serotype O1 | |
PCMB | 0.1 mM, complete inhibition | Vibrio cholerae serotype O1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
N-acetylglucosamine 6-phosphate | - |
Vibrio cholerae serotype O1 | |
4.6 | - |
N-acetylglucosamine 6-sulfate | - |
Vibrio cholerae serotype O1 | |
73 | - |
N-acetylglucosamine | - |
Vibrio cholerae serotype O1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 200% activation at 4 mM | Vibrio cholerae serotype O1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
4 * 45000, SDS-PAGE | Vibrio cholerae serotype O1 |
190000 | - |
gel filtration | Vibrio cholerae serotype O1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio cholerae serotype O1 | - |
Non-O1 | - |
Vibrio cholerae serotype O1 Non-O1 | - |
Non-O1 | - |
Purification (Comment) | Organism |
---|---|
- |
Vibrio cholerae serotype O1 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25.8 | - |
- |
Vibrio cholerae serotype O1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-glucosamine 6-phosphate + H2O | - |
Vibrio cholerae serotype O1 | D-glucosamine 6-phosphate + acetate | - |
? | |
N-acetyl-D-glucosamine 6-phosphate + H2O | - |
Vibrio cholerae serotype O1 Non-O1 | D-glucosamine 6-phosphate + acetate | - |
? | |
N-acetyl-D-glucosamine 6-sulfate + H2O | - |
Vibrio cholerae serotype O1 | D-glucosamine 6-sulfate + acetate | - |
? | |
N-acetyl-D-glucosamine 6-sulfate + H2O | - |
Vibrio cholerae serotype O1 Non-O1 | D-glucosamine 6-sulfate + acetate | - |
? | |
N-acetylglucosamine + H2O | - |
Vibrio cholerae serotype O1 | D-glucosamine + acetate | - |
? | |
N-acetylglucosamine + H2O | - |
Vibrio cholerae serotype O1 Non-O1 | D-glucosamine + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 45000, SDS-PAGE | Vibrio cholerae serotype O1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Vibrio cholerae serotype O1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
29 | - |
pH 6.0-8.0, 30 min, stable up to | Vibrio cholerae serotype O1 |
30 | - |
pH 6.0-8.0, 30 min, enzyme retains more than 70% of its activity | Vibrio cholerae serotype O1 |
45 | - |
pH 6.0-8.0, 30 min, complete loss of activity | Vibrio cholerae serotype O1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.97 | - |
N-acetylglucosamine 6-phosphate | - |
Vibrio cholerae serotype O1 | |
25 | - |
N-acetylglucosamine | - |
Vibrio cholerae serotype O1 | |
50 | - |
N-acetylglucosamine 6-sulfate | - |
Vibrio cholerae serotype O1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.5 | - |
Vibrio cholerae serotype O1 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | 30 min, stable up to 29°C, enzyme retains more than 70% of its activity at 30°C, complete loss of activity at 45°C | Vibrio cholerae serotype O1 |