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3.5.1.25: N-acetylglucosamine-6-phosphate deacetylase

This is an abbreviated version!
For detailed information about N-acetylglucosamine-6-phosphate deacetylase, go to the full flat file.

Word Map on EC 3.5.1.25

Reaction

N-acetyl-D-glucosamine 6-phosphate
+
H2O
=
D-glucosamine 6-phosphate
+
acetate

Synonyms

2-acetamido-2-deoxy-D-glucose-6-phosphate amidohydrolase, acetylaminodeoxyglucosephosphate acetylhydrolase, acetylglucosamine phosphate deacetylase, CaNAG2/Dac1, deacetylase, acetylglucosaminephosphate, EC 3.5.1.80, GlcNAc 6-P deacetylase, GlcNAc-6-phosphate deacetylase, GlnNAc6P deacetylase, Lmo0956, Lmo0956 protein, Lmo2108, Lmo2108 protein, MMNagA, MSNagA, N-acetyl-D-glucosamine-6-phosphate deacetylase, N-acetylglucosamine 6-phosphate deacetylase, N-acetylglucosamine-6-P deacetylase, N-acetylglucosamine-6-phosphate de-N-acetylase, N-acetylglucosamine-6-phosphate deacetylase, NAG2, NagA, NAGPase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.25 N-acetylglucosamine-6-phosphate deacetylase

Cloned

Cloned on EC 3.5.1.25 - N-acetylglucosamine-6-phosphate deacetylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Top10 cells
expression in Escherichia coli
-
gene lmo0956, genetic organization, phylogenetic analysis
gene lmo2108, genetic organization, phylogenetic analysis
gene nagA, recombinant expression in Escherichia coli strain BL21(DE3) from plasmid pET30DELTASE/nagA
-
NAG genes are expressed in Saccharomyces cerevisiae BY strains to study their effect on making novel carbon sources available for Saccharomyces cerevisiae
-
recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain C43(DE3)
recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli, coexpression with the Mtb GroES chaperone or pYUB1062
recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli, coexpression with the Mtb GroES chaperone or pYUB1062, or overexpression in Mycobacterium smegmatis