3.2.1.86: 6-phospho-beta-glucosidase

This is an abbreviated version!
For detailed information about 6-phospho-beta-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.86

3.2.1.86

phosphotransferase

beta-glucoside

arbutin

salicin

phosphoenolpyruvate-dependent

antiterminator

beta-glucoside-specific

mortiferum

phospho-beta-galactosidase

turanose

palatinose

glycosylhydrolase

trehalulose

leucrose

p-nitrophenyl-alpha-d-glucopyranoside

maltulose

Reaction

6-phospho-beta-D-glucosyl-(1->4)-D-glucose

Synonyms

6-phospho-alpha-glucosidase, 6-phospho-beta-glucosidase, AscB protein, bgl-2, BglA, BglA-2, BglA3, bglD, BglT, CelD, Cellobiose-6-phosphate hydrolase, Gan1D, LacG1, LacG2, More, P-beta-glc, PalH, Pbgl25-217, phospho-alpha-glucosidase, phospho-beta-glucosidase, phospho-beta-glucosidase A, phosphocellobiase, SPD_0247, SPy1599

ECTree

3 Hydrolases

3.2 Glycosylases

3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

3.2.1.86 6-phospho-beta-glucosidase

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Results	in table
4	Activating Compound
8988	AA Sequence
1	CAS Registry Number
13	Cloned(Commentary)
1	Cofactor
5	Crystallization (Commentary)
2	Expression
13	General Information
1	General Stability
22	Inhibitors
9	kcat/KM [mM/s]
1	Ki Value [mM]
67	KM Value [mM]
4	Localization
9	Metals/Ions
15	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
39	Organism
2	Pathway
48	PDB ID
13	pH Optimum
2	pH Range
1	pH Stability
1	pI Value
39	Protein Variants
15	Purification (Commentary)
2	Reaction
1	Reaction Type
39	Reference
5	Specific Activity [micromol/min/mg]
4	Storage Stability
101	Substrates and Products (Substrate)
13	Subunits
58	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
2	Temperature Range [°C]
3	Temperature Stability [°C]
24	Turnover Number [1/s]

Cloned

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Cloned on EC 3.2.1.86 - 6-phospho-beta-glucosidase

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2 entries

cloned from metagenome, library construction, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of wild-type and mutant His-tagged enzymes in Escherichia coli strain Rosetta (DE3)

uncultured organism

L7Y2Z6

731178

expressed in Escherichia coli

2 entries

expressed in Escherichia coli BL21(DE3) cells

2 entries

expressed in the beta-glucoside-negative strain Lactobacillus helveticus 3036(62), transformed strain is able to grow with arbutin, cellobiose, salicin and methyl-beta-glucoside as substrates

Lactobacillus delbrueckii

656717

gene palH, palH is present in a cluster of nine genes whose products may facilitate the transport, and metabolism of palatinose in this plant pathogen, phylogenetic analysis, expression of soluble His-tagged wild-type and mutant PalHs in Escherichia coli

Erwinia rhapontici

Q9AI65

705622

gene Spy1599, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)

Streptococcus pyogenes

Q1JK37

731055

gene SP_0578 or bglA-2, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)

Streptococcus pneumoniae

A0A0H2UP35

732101

LacG1, phylogenetic analysis

Lactobacillus gasseri

O06532

732252

LacG2, phylogenetic analysis, recombinant expression of His6-tagged enzyme in Lactococcus lactis strain MG1363

Lactobacillus gasseri

O06532

732252