3.2.1.86: 6-phospho-beta-glucosidase

This is an abbreviated version!
For detailed information about 6-phospho-beta-glucosidase, go to the full flat file.

Word Map on EC 3.2.1.86

3.2.1.86

phosphotransferase

beta-glucoside

arbutin

salicin

phosphoenolpyruvate-dependent

antiterminator

beta-glucoside-specific

mortiferum

phospho-beta-galactosidase

turanose

palatinose

glycosylhydrolase

trehalulose

leucrose

p-nitrophenyl-alpha-d-glucopyranoside

maltulose

Reaction

6-phospho-beta-D-glucosyl-(1->4)-D-glucose

Synonyms

6-phospho-alpha-glucosidase, 6-phospho-beta-glucosidase, AscB protein, bgl-2, BglA, BglA-2, BglA3, bglD, BglT, CelD, Cellobiose-6-phosphate hydrolase, Gan1D, LacG1, LacG2, More, P-beta-glc, PalH, Pbgl25-217, phospho-alpha-glucosidase, phospho-beta-glucosidase, phospho-beta-glucosidase A, phosphocellobiase, SPD_0247, SPy1599

ECTree

3 Hydrolases

3.2 Glycosylases

3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

3.2.1.86 6-phospho-beta-glucosidase

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Results	in table
4	Activating Compound
8988	AA Sequence
1	CAS Registry Number
13	Cloned(Commentary)
1	Cofactor
5	Crystallization (Commentary)
2	Expression
13	General Information
1	General Stability
22	Inhibitors
9	kcat/KM [mM/s]
1	Ki Value [mM]
67	KM Value [mM]
4	Localization
9	Metals/Ions
15	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
39	Organism
2	Pathway
48	PDB ID
13	pH Optimum
2	pH Range
1	pH Stability
1	pI Value
39	Protein Variants
15	Purification (Commentary)
2	Reaction
1	Reaction Type
39	Reference
5	Specific Activity [micromol/min/mg]
4	Storage Stability
101	Substrates and Products (Substrate)
13	Subunits
58	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
2	Temperature Range [°C]
3	Temperature Stability [°C]
24	Turnover Number [1/s]

Purification

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Purification on EC 3.2.1.86 - 6-phospho-beta-glucosidase

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3 entries

14 fold

Klebsiella aerogenes

136923, 136925

23 fold

Fusobacterium mortiferum

136927

63 fold

Escherichia coli

136924

HisTrap column chromatography

Streptococcus pyogenes

Q99YP9

750546

immobilized metal affinity chromatography

Streptococcus pneumoniae

750733

native LacG1 to homogeneity by ammonium sulfate fractionation, dialysis, anion exchange and hydroxyapatite chromatography, and ultrafiltration

Lactobacillus gasseri

O06532

732252

recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration

Streptococcus pyogenes

Q1JK37

731055

recombinant His6-tagged enzyme LacG2 to homogeneity by nickel affinity and adsorption chromatography, and ultrafiltration

Lactobacillus gasseri

O06532

732252

recombinant soluble His-tagged wild-type and mutant PalHs from Escherichia coli by ultracentrifugation, anion exchange chromatography, and gel filtration

Erwinia rhapontici

Q9AI65

705622

recombinant tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration

Streptococcus pneumoniae

A0A0H2UP35

732101

recombinant wild-type and mutant His-tagged enzymes from Escherichia coli strain Rosetta (DE3) by nickel afinity and anion exchange and again nickel affinity chromatography, followed by ultrafiltration

uncultured organism

L7Y2Z6

731178