Cloned (Comment) | Organism |
---|---|
gene Spy1599, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Streptococcus pyogenes |
Crystallization (Comment) | Organism |
---|---|
crystals with C2, P2 twinned, and P2 not twinned structures, hanging-drop vapour diffusion method, mixing equal volumes of 20 mg/ml protein with 0.1 M Bis-Tris propane, pH 7.5, 0.2 M sodium fluoride or sodium bromide, 20% PEG 3350 at 19°C, X-ray diffraction structure determination and analysis at 2.51-2.61 A resolution, three-dimensional structure analysis of the enzyme in a number of crystal forms complicated by unusual crystallographic twinning, the enzyme shows a classical (beta/alpha)8-barrel structure, molecular replacement method | Streptococcus pyogenes |
General Stability | Organism |
---|---|
SPy1599 is subject to degradation during both storage and crystallization experiments | Streptococcus pyogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by cyclohexylammonium or cyclohexylamine salt | Streptococcus pyogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Streptococcus pyogenes | |
0.73 | - |
4-nitrophenyl-beta-D-glucopyranoside 6-phosphate | pH 6.0, 37°C | Streptococcus pyogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes | Q1JK37 | gene Spy1599 | - |
Streptococcus pyogenes M1 GAS SF370 | Q1JK37 | gene Spy1599 | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration | Streptococcus pyogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O | - |
Streptococcus pyogenes | 4-nitrophenol + beta-D-glucose 6-phosphate | - |
? | |
4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O | - |
Streptococcus pyogenes M1 GAS SF370 | 4-nitrophenol + beta-D-glucose 6-phosphate | - |
? | |
additional information | the enzyme SPy1599 contains a phosphate-binding pocket in the active site and has specific 6-phospho-beta-glucosidase activity. No or very poor activity with 4-nitrophenyl derivatives of galactose, glucose, xylose, galactose 6-phosphate, N-acetyl-D-galactosamine, and N-acetyl-D-glusosamine | Streptococcus pyogenes | ? | - |
? | |
additional information | the enzyme SPy1599 contains a phosphate-binding pocket in the active site and has specific 6-phospho-beta-glucosidase activity. No or very poor activity with 4-nitrophenyl derivatives of galactose, glucose, xylose, galactose 6-phosphate, N-acetyl-D-galactosamine, and N-acetyl-D-glusosamine | Streptococcus pyogenes M1 GAS SF370 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme shows a classical (beta/alpha)8-barrel structure | Streptococcus pyogenes |
Synonyms | Comment | Organism |
---|---|---|
SPy1599 | - |
Streptococcus pyogenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Streptococcus pyogenes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.243 | - |
4-nitrophenyl-beta-D-glucopyranoside 6-phosphate | pH 6.0, 37°C | Streptococcus pyogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Streptococcus pyogenes |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycosyl hydrolase family 1, GH1 | Streptococcus pyogenes |
additional information | structure comparisons, active center and substrate specificity, overview | Streptococcus pyogenes |
physiological function | the enzyme SPy1599 is involved in the intracellular degradation of 6-phosphoglycosides, which are likely to originate from import through one of the organism's many phosphoenolpyruvate phosphotransfer systems | Streptococcus pyogenes |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.333 | - |
4-nitrophenyl-beta-D-glucopyranoside 6-phosphate | pH 6.0, 37°C | Streptococcus pyogenes |