3.2.1.86: 6-phospho-beta-glucosidase

This is an abbreviated version!
For detailed information about 6-phospho-beta-glucosidase, go to the full flat file.

Reaction

Synonyms

6-phospho-alpha-glucosidase, 6-phospho-beta-glucosidase, AscB protein, bgl-2, BglA, BglA-2, BglA3, bglD, BglT, CelD, Cellobiose-6-phosphate hydrolase, Gan1D, LacG1, LacG2, More, P-beta-glc, PalH, Pbgl25-217, phospho-alpha-glucosidase, phospho-beta-glucosidase, phospho-beta-glucosidase A, phosphocellobiase, SPD_0247, SPy1599

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.86 6-phospho-beta-glucosidase

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Results	in table
4	Activating Compound
8988	AA Sequence
1	CAS Registry Number
13	Cloned(Commentary)
1	Cofactor
5	Crystallization (Commentary)
2	Expression
13	General Information
1	General Stability
22	Inhibitors
9	kcat/KM [mM/s]
1	Ki Value [mM]
67	KM Value [mM]
4	Localization
9	Metals/Ions
15	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
39	Organism
2	Pathway
48	PDB ID
13	pH Optimum
2	pH Range
1	pH Stability
1	pI Value
39	Protein Variants
15	Purification (Commentary)
2	Reaction
1	Reaction Type
39	Reference
5	Specific Activity [micromol/min/mg]
4	Storage Stability
101	Substrates and Products (Substrate)
13	Subunits
58	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
2	Temperature Range [°C]
3	Temperature Stability [°C]
24	Turnover Number [1/s]

Crystallization

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Crystallization on EC 3.2.1.86 - 6-phospho-beta-glucosidase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

in complex with cellobiose-6-phosphate, 6-phospho-D-galactose or 6-phospho-D-glucose, hanging drop vapor diffusion method, using 16-22% (w/v) polyethylene glycol 8 K, 3% (w/v) methylpentanediol, 0.1 M imidazole buffer pH 6.5

Geobacillus stearothermophilus

W8QF82

750522

purified recombinant enzyme in apoform or complexed with thiocellobiose 6-phosphate, sitting drop vapour diffusion method, mixing of 0.001 ml of 10 mg/ml protein in 20 mM Tris-Cl, pH 8.0, 100 mM NaCl with 0.001 ml of reservoir solution containing 15% PEG 5000MME, and 0.1 M sodium citrate, pH 5.6, 16°C, X-ray diffraction structure determination and analysis at 2.0-2.4 A resolution, molecular replacement method

Streptococcus pneumoniae

A0A0H2UP35

732101

crystals with C2, P2 twinned, and P2 not twinned structures, hanging-drop vapour diffusion method, mixing equal volumes of 20 mg/ml protein with 0.1 M Bis-Tris propane, pH 7.5, 0.2 M sodium fluoride or sodium bromide, 20% PEG 3350 at 19°C, X-ray diffraction structure determination and analysis at 2.51-2.61 A resolution, three-dimensional structure analysis of the enzyme in a number of crystal forms complicated by unusual crystallographic twinning, the enzyme shows a classical (beta/alpha)8-barrel structure, molecular replacement method

Streptococcus pyogenes

Q1JK37

731055

in complex with 6-phospho-cyclophellitol, sitting drop vapor diffusion method, using 20% (w/v) PEG3350, NaBr 0.15 M, Bis-Tris propane 0.1 M, pH 7.5

Streptococcus pyogenes

Q99YP9

750546

hanging-drop vapor-diffusion method, crystal structure of the native selenomethionine-substituted enzyme (2.85 A resolution) and of the enzyme in complex with NAD+, Mn2+ and glucose 6-phosphate (2.55 A resolution)

Thermotoga maritima

Q9X108

666041