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3.2.1.65: levanase

This is an abbreviated version!
For detailed information about levanase, go to the full flat file.

Word Map on EC 3.2.1.65

Reaction

Glcpalpha(1-6)[Frufbeta(2-6)]n
+
H2O
=
Glcpalpha(1-6)[Frufbeta(2-6)]n-m
 +
[Frufbeta(2-6)]m

Synonyms

2,6-beta-D fructan fructohydrolase, 2,6-beta-D-fructan fructanohydrolase, 6-FEHs, endo-levanase, endolevanase, exo-levanase, exolevanase, fructan-6-exohydrolase, fructose-releasing exo-levanase, LeA, levan hydrolase, levanase, levansucrase, LevB1, LevP, LsdB, ORF477, SacB

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.65 levanase

Specific Activity

Specific Activity on EC 3.2.1.65 - levanase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.25
-
intracellular wild-type enzyme
0.3
-
BG4103-strain
0.33
-
extracellular mutant AD6 enzyme
0.4
-
QB167-strain
0.5
-
U/ml, inulin
0.52
-
extracellular mutant AD8 enzyme
0.69
-
intracellular mutant AD9 enzyme
0.71
-
extracellular mutant AD9 enzyme
0.73
-
intracellular mutant AD8 enzyme
0.75
-
intracellular mutant AD6 enzyme
1.8
purified recombinant enzyme, pH 6.0, 35°C
128
-
U/mg
14.24
-
extracellular wild-type enzyme
226.7
-
purified native enzyme, pH 6.5, 40°C
30.5
Pseudomonas sp. No. 43
-
U/mg
37.9
crude recombinant enzyme, pH 6.5, 50°C, substrate inulin
39.2
Pseudomonas strain K-52
-
-
4309
-
U/mg
58
-
levan, pH 5.0, at 30ºC
987
purified recombinant enzyme, pH 6.5, 50°C, substrate inulin
additional information
-
the levanase preparation exhibits an activity of 1116 U/ml towards levan