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Literature summary for 3.2.1.65 extracted from

  • Dahech, I.; Ben Ayed, H.; Belghith, K.S.; Belghith, H.; Mejdoub, H.
    Microbial production of levanase for specific hydrolysis of levan (2013), Int. J. Biol. Macromol., 60, 128-133.
    View publication on PubMed
Search for more literature for 3.2.1.65

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol slight activation at 75 mM uncultured bacterium

Inhibitors

Inhibitors Comment Organism Structure
EDTA slight inhibition at 75 mM uncultured bacterium
Fe2+ slight inhibition at 75 mM uncultured bacterium
Mg2+ slight inhibition at 75 mM uncultured bacterium
Zn2+ slight inhibition at 75 mM uncultured bacterium

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 uncultured bacterium
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ slight activation at 75 mM uncultured bacterium
NaCl slight activation at 75 mM uncultured bacterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
levan + H2O uncultured bacterium
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
uncultured bacterium
-
 isolated from a Tunisian thermal source
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 397fold from culture supernatant by ammonium sulfate fractionation, dialysis, gel filtration, and anion exchange chromatography uncultured bacterium

Source Tissue

Source Tissue Comment Organism Textmining
additional information maximum production of levanase activity is achieved after 36 h uncultured bacterium
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
226.7
-
 purified native enzyme, pH 6.5, 40°C uncultured bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
levan + H2O
-
 uncultured bacterium ?
-
 ?
levan + H2O
-
 uncultured bacterium levanbiose + D-fructose final products, thinlayer chromatography analysis ?
additional information the exo-acting enzyme is capable of hydrolyzing not only beta-2,6-fructosyl linkages, but also beta-2,1-fructosyl linkages uncultured bacterium ?
-
 ?

Synonyms

Synonyms Comment Organism
2,6-beta-D fructan fructohydrolase
-
 uncultured bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 uncultured bacterium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 activity range uncultured bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 crude extracellular enzyme, pH 6.4, stable up to uncultured bacterium
70
-
 crude extracellular enzyme, pH 6.4, inactivation uncultured bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
 uncultured bacterium