3.2.1.65: levanase
This is an abbreviated version!
For detailed information about levanase, go to the full flat file.
Word Map on EC 3.2.1.65
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3.2.1.65
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levans
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inulin
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isecp1
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extended-spectrum
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esbls
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integrons
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levansucrase
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blactx-m-1
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esbl-producing
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esbl-positive
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fructooligosaccharides
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inulinase
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levanbiose
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phylogroups
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fructosyl
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synthesis
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blaoxa-1
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levan-type
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cephalosporin-resistant
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nutrition
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agriculture
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blatem-1b
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medicine
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fructanase
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dfra17-aada5
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ctx-m-1
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ctx-m-15-producing
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ctx-m-producing
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fructosyltransferase
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food industry
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analysis
- 3.2.1.65
- levans
- inulin
-
isecp1
-
extended-spectrum
-
esbls
- integrons
- levansucrase
-
blactx-m-1
-
esbl-producing
-
esbl-positive
- fructooligosaccharides
- inulinase
- levanbiose
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phylogroups
-
fructosyl
- synthesis
- blaoxa-1
-
levan-type
-
cephalosporin-resistant
- nutrition
- agriculture
-
blatem-1b
- medicine
- fructanase
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dfra17-aada5
- ctx-m-1
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ctx-m-15-producing
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ctx-m-producing
- fructosyltransferase
- food industry
- analysis
Reaction
Synonyms
2,6-beta-D fructan fructohydrolase, 2,6-beta-D-fructan fructanohydrolase, 6-FEHs, endo-levanase, endolevanase, exo-levanase, exolevanase, fructan-6-exohydrolase, fructose-releasing exo-levanase, LeA, levan hydrolase, levanase, levansucrase, LevB1, LevP, LsdB, ORF477, SacB
ECTree
3.2.1.65 levanaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.2.1.65 - levanase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
D(+)-raffinose + H2O
beta-D-fructose + alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranoside
Erwinia herbicola levan + H2O
D-fructose + fructooligosacchrides
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-
-
-
?
high molecular weight levan + H2O
D-fructose + fructooligosaccharides DP2-10
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-
-
-
?
levan + H2O
levanbiose + D-fructose
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final products, thinlayer chromatography analysis
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?
low molecular weight levan + H2O
D-fructose + fructooligosaccharides DP2-10
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-
-
-
?
D(+)-raffinose + H2O
beta-D-fructose + alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranoside
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-
-
-
?
D(+)-raffinose + H2O
beta-D-fructose + alpha-D-galactopyranosyl-(1->6)-alpha-D-glucopyranoside
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-
-
-
?
high molecular weight levan + H2O
D-fructose + ?
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minor activity
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-
?
inulin + H2O
D-fructose + ?
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without formation of intermediate oligofructans
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-
?
inulin + H2O
D-fructose + ?
without formation of intermediate oligofructans
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-
?
inulin + H2O
D-fructose + ?
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without formation of intermediate oligofructans
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-
?
inulin + H2O
D-fructose + ?
without formation of intermediate oligofructans
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-
?
levan + H2O
?
-
commercial Erwinia herbicola levan and levan from Leuconostoc mesenteroides DSM 20343-fermented fava bean doughs, combined activity of exoinulinase/levanase
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-
?
levan + H2O
D-fructose + ?
4fold higher rate of hydrolysis compared to inulin, without formation of intermediate oligofructans, no activity with D(+)-melezitose
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-
?
levan + H2O
D-fructose + ?
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without formation of intermediate oligofructans, no activity with D(+)-melezitose
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-
?
levan + H2O
D-fructose + ?
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without formation of intermediate oligofructans, no activity with D(+)-melezitose
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-
?
levan + H2O
D-fructose + ?
4fold higher rate of hydrolysis compared to inulin, without formation of intermediate oligofructans, no activity with D(+)-melezitose
-
-
?
levan + H2O
fructose + beta-1,2-oligofructans
-
-
-
?
levan + H2O
fructose + beta-1,2-oligofructans
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-
-
?
levan + H2O
O-beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranoside + ?
almost no differences in specific activity at 1% w/v levan are found when LevB1 is assayed with levans obtained from the levansucrases of Bacillus subtilis or Leuconostoc mesenteroides ATCC 8293, but it is 10fold less active toward levan from Leuconostoc mesenteroides NRRL-B512 levansucrase
major product
-
?
levan + H2O
O-beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranoside + ?
almost no differences in specific activity at 1% w/v levan are found when LevB1 is assayed with levans obtained from the levansucrases of Bacillus subtilis or Leuconostoc mesenteroides ATCC 8293, but it is 10fold less active toward levan from Leuconostoc mesenteroides NRRL-B512 levansucrase
major product
-
?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
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-
-
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?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
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-
-
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?
sucrose + H2O
D-fructose + D-glucose
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-
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?
additional information
?
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-
in order to develop a specific analysis for levan in food matrices, a Paenibacillus amylolyticus endolevanase (EC 3.2.1.65) is combined with exoinulinase (EC 3.2.1.80) for levan hydrolysis achieving about 80% hydrolysis yield, method evaluation, and modelling. The levanase preparation exhibits an activity of 1116 U/ml towards levan and no activity towards inulin as determined by the reducing value method
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-
?
additional information
?
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a levanase with high inulinase activity. The strain ATCC 14580 efficiently produces 2,3-butanediol from fructose at 50°C
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-
?
additional information
?
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a levanase with high inulinase activity. The strain ATCC 14580 efficiently produces 2,3-butanediol from fructose at 50°C
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-
?
additional information
?
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the levansucrase-endolevanase fusion enzyme LevB1SacB or both enzymes (levanase LevB1 and levansucrase SacB) at equimolar concentrations in simultaneous reactions result in an optimal, wide and diverse L-FOS profile, including 6-kestose, levanbiose and blastose among other L-FOS and 1-kestose, which accumulates as by-product of SacB levan synthesis
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-
?
additional information
?
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a levanase with high inulinase activity. The strain ATCC 14580 efficiently produces 2,3-butanediol from fructose at 50°C
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-
?
additional information
?
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enzyme reactions with levan in the presence of D-glucose and D-fructose results in the production of a wide variety of oligosaccharides such as blastose, levanobiose, inulobiose, 1-kestose and 6-kestose
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-
?
additional information
?
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enzyme reactions with levan in the presence of D-glucose and D-fructose results in the production of a wide variety of oligosaccharides such as blastose, levanobiose, inulobiose, 1-kestose and 6-kestose
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-
?
additional information
?
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the exo-acting enzyme is capable of hydrolyzing not only beta-2,6-fructosyl linkages, but also beta-2,1-fructosyl linkages
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-
?
