3.1.3.32: polynucleotide 3'-phosphatase
This is an abbreviated version!
For detailed information about polynucleotide 3'-phosphatase, go to the full flat file.
Word Map on EC 3.1.3.32
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3.1.3.32
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3'-phosphate
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exonuclease
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5'-kinase
-
ape1
-
5\'-hydroxyl
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3'-blocking
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xrcc4
-
end-healing
-
apraxia
-
apurinic
-
aprataxin
-
diesterase
-
end-processing
-
3'-phosphorylated
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strand-breaks
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3'-phosphoesterase
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3'-phosphoglycolate
-
phosphoesterase
-
tyrosyl-dna
-
analysis
-
ap-endonuclease
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pharmacology
-
medicine
- 3.1.3.32
- 3'-phosphate
-
exonuclease
-
5'-kinase
- ape1
-
5\'-hydroxyl
-
3'-blocking
- xrcc4
-
end-healing
- apraxia
-
apurinic
- aprataxin
- diesterase
-
end-processing
-
3'-phosphorylated
-
strand-breaks
-
3'-phosphoesterase
-
3'-phosphoglycolate
-
phosphoesterase
-
tyrosyl-dna
- analysis
- ap-endonuclease
- pharmacology
- medicine
Reaction
Synonyms
2'(3')-polynucleotidase, 3' phosphatase, 3'-phosphatase, 3'-phosphatase/5'-OH kinase, 5' polynucleotidekinase 3' phosphatase, 5'-polynucleotide kinase/3'-phosphatase, APLF, APTX/PNKP-like factor, AtZDP, chromatin 3'-phosphatase, deoxyribonucleate 3'-phosphatase, DNA 3'-phosphatase, phosphatase, polynucleotide 3'-, Pnk1, PNKP, polynucleotide kinase 3'-phosphatase, polynucleotide kinase phosphatase, polynucleotide kinase-phosphatase, polynucleotide kinase/phosphatase, RNA ligase 1, Rnl1, SNQI-PNK, T4 polynucleotide kinase/phosphatase, Tpp1
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Reference
Reference on EC 3.1.3.32 - polynucleotide 3'-phosphatase
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Habraken, Y.; Verly, W.G.
Further purification and characterization of the DNA 3-phosphatase from rat-liver chromatin which is also a polynucleotide 5-hydroxyl kinase
Eur. J. Biochem.
171
59-66
1988
Rattus norvegicus
Habraken, Y.; Verly, W.G.
Chromatin 3-phosphatase/5-OH kinase cannot transfer phosphate from 3 to 5 across a strand nick in DNA
Nucleic Acids Res.
14
8103-8110
1986
Rattus norvegicus
Habraken, Y.; Verly, W.G.
The DNA 3-phosphatase and 5-hydroxyl kinase of rat liver chromatin
FEBS Lett.
160
46-50
1983
Rattus norvegicus
Pheiffer, B.H.; Zimmerman, S.B.
3-Phosphatase activity of the DNA kinase from rat liver
Biochem. Biophys. Res. Commun.
109
1297-1302
1982
Rattus norvegicus
Sirotkin, K.; Cooley, W.; Runnels, J.; Snyder, L.R.
A role in true-late gene expression for the T4 bacteriophage 5 polynucleotide kinase 3 phosphatase
J. Mol. Biol.
123
221-233
1978
Tequatrovirus T4
Jilani, A.; Ramotar, D.; Slack, C.; Ong, C.; Yang, X.M.; Scherer, S.W.; Lasko, D.D.
Molecular cloning of the human gene, PNKP, encoding a polynucleotide kinase 3-phosphatase and evidence for its role in repair of DNA strand breaks caused by oxidative damage
J. Biol. Chem.
274
24176-24186
1999
Homo sapiens (Q96T60), Homo sapiens
Jilani, A.; Slack, C.; Matheos, D.; Zannis-Hadjopoulos, M.; Lasko, D.D.
Purification of a polynucleotide kinase from calf thymus, comparison of its 3-phosphatase domain with T4 polynucleotide kinase, and investigation of its effect on DNA replication in vitro
J. Cell. Biochem.
73
188-203
1999
Bos taurus
Wang, L.K.; Shuman, S.
Mutational analysis defines the 5'-kinase and 3'-phosphatase active sites of T4 polynucleotide kinase
Nucleic Acids Res.
30
1073-1080
2002
Tequatrovirus T4
Jilani, A.; Ramotar, D.
Purification and partial characterization of a DNA 3'-phosphatase from Schizosaccharomyces pombe
Biochemistry
41
7688-7694
2002
Schizosaccharomyces pombe (O13911), Schizosaccharomyces pombe
Deshpande, R.A.; Wilson, T.E.
Identification of DNA 3'-phosphatase active site residues and their differential role in DNA binding, mg(2+) coordination, and catalysis
Biochemistry
43
8579-8589
2004
Saccharomyces cerevisiae
Vance, J.R.; Wilson, T.E.
Uncoupling of 3'-phosphatase and 5'-kinase functions in budding yeast. Characterization of Saccharomyces cerevisiae DNA 3'-phosphatase (TPP1)
J. Biol. Chem.
276
15073-15081
2001
Saccharomyces mikatae (Q9HET9), Saccharomyces mikatae
Petrucco, S.; Volpi, G.; Bolchi, A.; Rivetti, C.; Ottonello, S.
A nick-sensing DNA 3'-repair enzyme from Arabidopsis
J. Biol. Chem.
277
23675-23683
2002
Arabidopsis thaliana (Q84JE8)
Meijer, M.; Karimi-Busheri, F.; Huang, T.Y.; Weinfeld, M.; Young, D.
Pnk1, a DNA kinase/phosphatase required for normal response to DNA damage by g-radiation or camptothecin in Schizosaccharomyces pombe
J. Biol. Chem.
277
4050-4055
2002
Schizosaccharomyces pombe
Karumbati, A.S.; Deshpande, R.A.; Jilani, A.; Vance, J.R.; Ramotar, D.; Wilson, T.E.
The role of yeast DNA 3'-phosphatase Tpp1 and rad1/Rad10 endonuclease in processing spontaneous and induced base lesions
J. Biol. Chem.
278
31434-31443
2003
Saccharomyces cerevisiae
Zhu, H.; Yin, S.; Shuman, S.
Characterization of polynucleotide kinase/phosphatase enzymes from Mycobacteriophages omega and Cjw1 and vibriophage KVP40
J. Biol. Chem.
279
26358-26369
2004
Omegavirus omega, Kostyavirus CJW1
Martins, A.; Shuman, S.
Characterization of a baculovirus enzyme with RNA ligase, polynucleotide 5'-Kinase, and polynucleotide 3'-phosphatase activities
J. Biol. Chem.
279
18220-18231
2004
unidentified baculovirus
Blondal, T.; Hjorleifsdottir, S.; Aevarsson, A.; Fridjonsson, O.H.; Skirnisdottir, S.; Wheat, J.O.; Hermannsdottir, A.G.; Hreggvidsson, G.O.; Smith, A.V.; Kristjansson, J.K.
Characterization of a 5'-polynucleotide kinase/3'-phosphatase from bacteriophage RM378
J. Biol. Chem.
280
5188-5194
2005
Rhodothermus phage RM378
Keppetipola, N.; Shuman, S.
Distinct enzymic functional groups are required for the phosphomonoesterase and phosphodiesterase activities of Clostridium thermocellum polynucleotide kinase/phosphatase
J. Biol. Chem.
281
19251-19259
2006
Acetivibrio thermocellus
Zuber, S.; Ngom-Bru, C.; Barretto, C.; Bruttin, A.; Brussow, H.; Denou, E.
Genome analysis of phage JS98 defines a fourth major subgroup of T4-like phages in Escherichia coli
J. Bacteriol.
189
8206-8214
2007
Escherichia phage JS98 (A8R9L0)
Zhu, H.; Shuman, S.
Substrate specificity and structure-function analysis of the 3-phosphoesterase component of the bacterial NHEJ protein, DNA ligase D
J. Biol. Chem.
281
13873-13881
2006
Tequatrovirus T4, Saccharomyces cerevisiae
Keppetipola, N.; Shuman, S.
Characterization of the 2,3 cyclic phosphodiesterase activities of Clostridium thermocellum polynucleotide kinase-phosphatase and bacteriophage lambda phosphatase
Nucleic Acids Res.
35
7721-7732
2007
Acetivibrio thermocellus
Zhu, H.; Smith, P.; Wang, L.K.; Shuman, S.
Structure-function analysis of the 3' phosphatase component of T4 polynucleotide kinase/phosphatase
Virology
366
126-136
2007
Tequatrovirus T4
Murphy, G.; Sansbury, L.S.; Bergen, A.W.; Wang, Z.; Schatzkin, A.; Lehman, T.; Kalidindi, A.; Modali, R.; Lanza, E.
Polynucleotide kinase 3' phosphatase variant, dietary variables and risk of adenoma recurrence in the Polyp Prevention Trial
Eur. J. Cancer Prev.
17
287-290
2008
Homo sapiens (Q96T60)
Freschauf, G.K.; Karimi-Busheri, F.; Ulaczyk-Lesanko, A.; Mereniuk, T.R.; Ahrens, A.; Koshy, J.M.; Rasouli-Nia, A.; Pasarj, P.; Holmes, C.F.; Rininsland, F.; Hall, D.G.; Weinfeld, M.
Identification of a small molecule inhibitor of the human DNA repair enzyme polynucleotide kinase/phosphatase
Cancer Res.
69
7739-7746
2009
Homo sapiens, Mus musculus, Schizosaccharomyces pombe
Freschauf, G.K.; Mani, R.S.; Mereniuk, T.R.; Fanta, M.; Virgen, C.A.; Dianov, G.L.; Grassot, J.M.; Hall, D.G.; Weinfeld, M.
Mechanism of action of an imido-piperidine inhibitor of human polynucleotide kinase/phosphatase
J. Biol. Chem.
285
2351-2360
2010
Homo sapiens
Shen, J.; Gilmore, E.C.; Marshall, C.A.; Haddadin, M.; Reynolds, J.J.; Eyaid, W.; Bodell, A.; Barry, B.; Gleason, D.; Allen, K.; Ganesh, V.S.; Chang, B.S.; Grix, A.; Hill, R.S.; Topcu, M.; Caldecott, K.W.; Barkovich, A.J.; Walsh, C.A.
Mutations in PNKP cause microcephaly, seizures and defects in DNA repair
Nat. Genet.
42
245-249
2010
Homo sapiens
Ali, A.A.; Jukes, R.M.; Pearl, L.H.; Oliver, A.W.
Specific recognition of a multiply phosphorylated motif in the DNA repair scaffold XRCC1 by the FHA domain of human PNK
Nucleic Acids Res.
37
1701-1712
2009
Homo sapiens (Q96T60)
Song, C.; Zhang, C.; Zhao, M.
Development of a high-throughput screening platform for DNA 3'-phosphatases and their inhibitors based on a universal molecular beacon and quantitative real-time PCR
Chem. Asian J.
5
1146-1151
2010
Tequatrovirus T4
Mani, R.S.; Yu, Y.; Fang, S.; Lu, M.; Fanta, M.; Zolner, A.E.; Tahbaz, N.; Ramsden, D.A.; Litchfield, D.W.; Lees-Miller, S.P.; Weinfeld, M.
Dual modes of interaction between XRCC4 and polynucleotide kinase/phosphatase: implications for nonhomologous end joining
J. Biol. Chem.
285
37619-37629
2010
Plasmodium falciparum
Weinfeld, M.; Mani, R.S.; Abdou, I.; Aceytuno, R.D.; Glover, J.N.
Tidying up loose ends: the role of polynucleotide kinase/phosphatase in DNA strand break repair
Trends Biochem. Sci.
36
262-271
2011
Homo sapiens, Mus musculus
Kashkina, E.; Qi, T.; Weinfeld, M.; Young, D.
Polynucleotide kinase/phosphatase, Pnk1, is involved in base excision repair in Schizosaccharomyces pombe
DNA Repair
11
676-683
2012
Schizosaccharomyces pombe
Siribal, S.; Weinfeld, M.; Karimi-Busheri, F.; Mark Glover, J.N.; Bernstein, N.K.; Aceytuno, D.; Chavalitshewinkoon-Petmitr, P.
Molecular characterization of Plasmodium falciparum putative polynucleotide kinase/phosphatase
Mol. Biochem. Parasitol.
180
1-7
2011
Plasmodium falciparum
Tahbaz, N.; Subedi, S.; Weinfeld, M.
Role of polynucleotide kinase/phosphatase in mitochondrial DNA repair
Nucleic Acids Res.
40
3484-3495
2012
Homo sapiens
Liu, X.; Ge, J.; Wang, X.; Wu, Z.; Shen, G.; Yu, R.
Development of a highly sensitive sensing platform for T4 polynucleotide kinase phosphatase and its inhibitors based on WS2 nanosheets
Anal. Methods
6
7212-7217
2014
Tequatrovirus T4
-
Jiang, H.X.; Kong, D.M.; Shen, H.X.
Amplified detection of DNA ligase and polynucleotide kinase/phosphatase on the basis of enrichment of catalytic G-quadruplex DNAzyme by rolling circle amplification
Biosens. Bioelectron.
55
133-138
2014
Tequatrovirus T4
Della-Maria, J.; Hegde, M.L.; McNeill, D.R.; Matsumoto, Y.; Tsai, M.S.; Ellenberger, T.; Wilson, D.M.; Mitra, S.; Tomkinson, A.E.
The interaction between polynucleotide kinase phosphatase and the DNA repair protein XRCC1 is critical for repair of DNA alkylation damage and stable association at DNA damage sites
J. Biol. Chem.
287
39233-39244
2012
Homo sapiens
Shirodkar, P.; Fenton, A.L.; Meng, L.; Koch, C.A.
Identification and functional characterization of a Ku-binding motif in aprataxin polynucleotide kinase/phosphatase-like factor (APLF)
J. Biol. Chem.
288
19604-19613
2013
Homo sapiens
Das, U.; Shuman, S.
Mechanism of RNA 2,3-cyclic phosphate end healing by T4 polynucleotide kinase-phosphatase
Nucleic Acids Res.
41
355-365
2013
Tequatrovirus T4
Ma, C.; Jin, S.; Wang, J.; Wang, K.; Liu, H.; Wu, K.
A fluorescence-based assay for T4 polynucleotide kinase/phosphatase activity based on a terminal transferase-aided photoinduced electron transfer strategy
Anal. Methods
8
1989-1994
2016
Tequatrovirus T4 (P06855)
-
Havali-Shahriari, Z.; Weinfeld, M.; Glover, J.N.
Characterization of DNA substrate binding to the phosphatase domain of the DNA repair enzyme polynucleotide kinase/phosphatase
Biochemistry
56
1737-1745
2017
Mus musculus (Q9JLV6)
Shimada, M.; Dumitrache, L.C.; Russell, H.R.; McKinnon, P.J.
Polynucleotide kinase-phosphatase enables neurogenesis via multiple DNA repair pathways to maintain genome stability
EMBO J.
34
2465-2480
2015
Mus musculus (Q9JLV6), Mus musculus
Zhang, X.; Liu, Q.; Jin, Y.; Li, B.
Determination of the activity of T4 polynucleotide kinase phosphatase by exploiting the sequence-dependent fluorescence of DNA-templated copper nanoclusters
Microchim. Acta
186
003
2019
Tequatrovirus T4 (P06855)
Chatterjee, A.; Saha, S.; Chakraborty, A.; Silva-Fernandes, A.; Mandal, S.M.; Neves-Carvalho, A.; Liu, Y.; Pandita, R.K.; Hegde, M.L.; Hegde, P.M.; Boldogh, I.; Ashizawa, T.; Koeppen, A.H.; Pandita, T.K.; Maciel, P.; Sarkar, P.S.; Hazra, T.K.
The role of the mammalian DNA end-processing enzyme polynucleotide kinase 3-phosphatase in spinocerebellar ataxia type 3 pathogenesis
PLoS Genet.
11
e1004749
2015
Homo sapiens, Mus musculus (Q9JLV6), Mus musculus
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