3.1.3.32: polynucleotide 3'-phosphatase
This is an abbreviated version!
For detailed information about polynucleotide 3'-phosphatase, go to the full flat file.
Word Map on EC 3.1.3.32
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3.1.3.32
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3'-phosphate
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exonuclease
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5'-kinase
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ape1
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5\'-hydroxyl
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3'-blocking
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xrcc4
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end-healing
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apraxia
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apurinic
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aprataxin
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diesterase
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end-processing
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3'-phosphorylated
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strand-breaks
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3'-phosphoesterase
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3'-phosphoglycolate
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phosphoesterase
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tyrosyl-dna
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analysis
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ap-endonuclease
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pharmacology
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medicine
- 3.1.3.32
- 3'-phosphate
-
exonuclease
-
5'-kinase
- ape1
-
5\'-hydroxyl
-
3'-blocking
- xrcc4
-
end-healing
- apraxia
-
apurinic
- aprataxin
- diesterase
-
end-processing
-
3'-phosphorylated
-
strand-breaks
-
3'-phosphoesterase
-
3'-phosphoglycolate
-
phosphoesterase
-
tyrosyl-dna
- analysis
- ap-endonuclease
- pharmacology
- medicine
Reaction
Synonyms
2'(3')-polynucleotidase, 3' phosphatase, 3'-phosphatase, 3'-phosphatase/5'-OH kinase, 5' polynucleotidekinase 3' phosphatase, 5'-polynucleotide kinase/3'-phosphatase, APLF, APTX/PNKP-like factor, AtZDP, chromatin 3'-phosphatase, deoxyribonucleate 3'-phosphatase, DNA 3'-phosphatase, phosphatase, polynucleotide 3'-, Pnk1, PNKP, polynucleotide kinase 3'-phosphatase, polynucleotide kinase phosphatase, polynucleotide kinase-phosphatase, polynucleotide kinase/phosphatase, RNA ligase 1, Rnl1, SNQI-PNK, T4 polynucleotide kinase/phosphatase, Tpp1
ECTree
3.1.3.32 polynucleotide 3'-phosphataseAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.1.3.32 - polynucleotide 3'-phosphatase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3'-phospho-5'-hydroxy-ATTCGTGTGAGAAAACCCAACCCGCCCTACCCAAAAGTCAGATGA + H2O
?
Tequatrovirus T4
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the enzyme mediates 5'-phosphorylation of ATTCGTGTGAGAAAACCCAACCCGCCCTACCCAAAAGTCAGATGA
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-
?
3'-phospho-5'-hydroxy-poly(dT,dA) + H2O
3'-hydroxy-5'-phospho-poly(dT,dA)
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poly(dT,dA) with a 3' phosphate and and 5' OH group, preparation of substrate, presence of ATP
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?
a RNA 2',3'-cyclic phosphate end + H2O
a RNA 3'-phosphate + phosphate
Tequatrovirus T4
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the enzyme also has RNA 2'-phosphatase activity
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-
?
bis-p-nitrophenyl phosphate + H2O
p-nitrophenyl phosphate + phosphate
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-
-
-
?
d(TTTAATCAATTGCGACCCp) + H2O
phosphate + d(TTTAATCAATTGCGACCC)
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reaction with the end-healing domain Rnl1-(394-694), that consists of a proximal 5'-kinase module with an essential P-loop motif (404GSGKS408) and a distal 3'-phosphatase module with an essential acylphosphatase motif (560DLDGT564)
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-
?
DNA with 5'-hydroxyl ends + ATP
DNA with 5'-phosphate ends + ADP
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-
-
?
FAM-5'-TAGAGAGAGAGAGAGAGAGAGAGCGCA CCTAAAGGGTGCG-phospho-3' + H2O
FAM-5'-TAGAGAGAGAGAGAGAGAGAGAGCGCA CCTAAAGGGTGCG-hydroxyl-3' + phosphate
Tequatrovirus T4
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the primer probe can be dephosphorylated by the enzyme into a 3'-hydroxyl end
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-
?
oligodeoxyadenylate 3'-phosphate + H2O
oligodeoxyadenylate + phosphate
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-
-
?
3'-phopsho-5'-hydroxy-DNA
?
role in repair of DNA strand breaks caused by oxidative damage
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-
?
3'-phopsho-5'-hydroxy-DNA
?
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the enzyme is capable of converting a 3'-phosphate/5'-OH strand of nicked DNA, which is unreparable by DNA ligase into a reparable 3'-OH/5'-phosphate nick in double stranded DNA
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-
?
additional information
?
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not active towards dimethyl-p-nitrophenyl phosphate, thymidine 5'-monophosphate-p-nitrophenyl ester, and p-nitrophenyl phosphorylcholine
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-
?
additional information
?
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structure of the human PNKP FHA domain bound to a doubly phosphorylated phosphopeptide derived from XRCC1, overview
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-
?
additional information
?
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the enzyme acts on mitochondrial DNA and interacts with the mitochondrial proteins, overview
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-
?
additional information
?
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polynucleotide kinase/phosphatase is a bifunctional enzyme that can phosphorylate the 5'-OH termini and dephosphorylate the 3'-phosphate termini of DNA. Recombinant GFP-tagged PfPNKP dephosphorylates single-stranded substrates or double-stranded substrates with a short 3-single-stranded overhang, but not double-stranded substrates that mimicked single-strand breaks
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?
additional information
?
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polynucleotide kinase/phosphatase is a bifunctional enzyme that can phosphorylate the 5'-OH termini and dephosphorylate the 3'-phosphate termini of DNA. 3'-Phosphatase activity is detected, and PfPNKP dephosphorylates single-stranded substrates or double-stranded substrates with a short 3'-single-stranded overhang, but not double-stranded substrates that mimick single-strand breaks. But the recombinant PfPNKP does not reveal 5'-kinase activity
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-
?
additional information
?
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recombinant enzyme shows no activity with micrococcal nuclease digested DNA, nicked DNA, GC and AT rich 20-, 10-, 8-, 5-base oligonucleotides, and polyA that contain 5'-hydroxyl termini
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?
additional information
?
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repair of endogenously created 3-phosphate, involved in Rad1/Rad10 salvage pathway
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?
additional information
?
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removes 3-phosphates from nicks and single-nucleotide gaps with equal efficiency, inactive on single-stranded oligonucleotides
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?
additional information
?
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removes 3-phosphates from nicks and single-nucleotide gaps with equal efficiency, inactive on single-stranded oligonucleotides
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?
additional information
?
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acts in the repair of damaged DNA
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?
additional information
?
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acts in the repair of damaged DNA
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?
additional information
?
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Tequatrovirus T4
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polynucleotide kinase/phosphatase is a bifunctional enzyme with 5'-kinase and 3'-phosphatase activities which can generate 5'-phosphate and 3'-hydroxyl groups at damaged DNA termini, thus permitting subsequent repair proteins to replace missing nucleotides and rejoin broken strands
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?
