3.1.3.32: polynucleotide 3'-phosphatase
This is an abbreviated version!
For detailed information about polynucleotide 3'-phosphatase, go to the full flat file.
Word Map on EC 3.1.3.32
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3.1.3.32
-
3'-phosphate
-
exonuclease
-
5'-kinase
-
ape1
-
5\'-hydroxyl
-
3'-blocking
-
xrcc4
-
end-healing
-
apraxia
-
apurinic
-
aprataxin
-
diesterase
-
end-processing
-
3'-phosphorylated
-
strand-breaks
-
3'-phosphoesterase
-
3'-phosphoglycolate
-
phosphoesterase
-
tyrosyl-dna
-
analysis
-
ap-endonuclease
-
pharmacology
-
medicine
- 3.1.3.32
- 3'-phosphate
-
exonuclease
-
5'-kinase
- ape1
-
5\'-hydroxyl
-
3'-blocking
- xrcc4
-
end-healing
- apraxia
-
apurinic
- aprataxin
- diesterase
-
end-processing
-
3'-phosphorylated
-
strand-breaks
-
3'-phosphoesterase
-
3'-phosphoglycolate
-
phosphoesterase
-
tyrosyl-dna
- analysis
- ap-endonuclease
- pharmacology
- medicine
Reaction
Synonyms
2'(3')-polynucleotidase, 3' phosphatase, 3'-phosphatase, 3'-phosphatase/5'-OH kinase, 5' polynucleotidekinase 3' phosphatase, 5'-polynucleotide kinase/3'-phosphatase, APLF, APTX/PNKP-like factor, AtZDP, chromatin 3'-phosphatase, deoxyribonucleate 3'-phosphatase, DNA 3'-phosphatase, phosphatase, polynucleotide 3'-, Pnk1, PNKP, polynucleotide kinase 3'-phosphatase, polynucleotide kinase phosphatase, polynucleotide kinase-phosphatase, polynucleotide kinase/phosphatase, RNA ligase 1, Rnl1, SNQI-PNK, T4 polynucleotide kinase/phosphatase, Tpp1
ECTree
3.1.3.32 polynucleotide 3'-phosphataseAdvanced search results
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results (KM Value
KM Value on EC 3.1.3.32 - polynucleotide 3'-phosphatase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
67
p-Nitrophenyl phenylphosphonate
-
wild type enzyme, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
3.9
2',3'-cAMP
-
mutant enzyme H376D, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
4.7
2',3'-cAMP
-
mutant enzyme H376N, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
6.6
2',3'-cAMP
-
mutant enzyme D236N, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
7.8
2',3'-cAMP
-
mutant enzyme D236A, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
18
2',3'-cAMP
-
mutant enzyme D236E, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
18
2',3'-cAMP
-
mutant enzyme D392E, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
18
2',3'-cAMP
-
wild type enzyme, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
20
2',3'-cAMP
-
mutant enzyme H189D, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
24
2',3'-cAMP
-
mutant enzyme H323Q, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
28
2',3'-cAMP
-
mutant enzyme H189E, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
29
2',3'-cAMP
-
mutant enzyme H189A, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
31
2',3'-cAMP
-
mutant enzyme D392N, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
32
2',3'-cAMP
-
mutant enzyme H323A, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
60
2',3'-cAMP
-
mutant enzyme R237Q, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
62
2',3'-cAMP
-
mutant enzyme D233E, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
77
2',3'-cAMP
-
mutant enzyme R237A, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
100
2',3'-cAMP
-
mutant enzyme D392A, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
100
2',3'-cAMP
-
mutant enzyme R237K, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
1.6
bis-p-nitrophenyl phosphate
-
mutant enzyme D236A, at 45°C, in 50 mM Tris-HCl (pH 8.0) 0.5 mM NiCl2
2.2
bis-p-nitrophenyl phosphate
-
mutant enzyme D236N, at 45°C, in 50 mM Tris-HCl (pH 8.0) 0.5 mM NiCl2
3.4
bis-p-nitrophenyl phosphate
-
mutant enzyme H264A, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2
3.6
bis-p-nitrophenyl phosphate
-
mutant enzyme H189D, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
5
bis-p-nitrophenyl phosphate
-
mutant enzyme D236N, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
5
bis-p-nitrophenyl phosphate
-
mutant enzyme D392E, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
5
bis-p-nitrophenyl phosphate
-
mutant enzyme H376D, at 45°C, in 50 mM Tris-HCl (pH 8.0) 0.5 mM MnCl2
6.3
bis-p-nitrophenyl phosphate
-
wild type enzyme, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2
6.6
bis-p-nitrophenyl phosphate
-
mutant enzyme H189A, at 45°C, in 50 mM Tris-HCl (pH 8.0) 0.5 mM MnCl2
7.2
bis-p-nitrophenyl phosphate
-
mutant enzyme H376N, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
7.4
bis-p-nitrophenyl phosphate
-
mutant enzyme D392N, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
9.6
bis-p-nitrophenyl phosphate
-
mutant enzyme H264N, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2
11
bis-p-nitrophenyl phosphate
-
mutant enzyme H264Q, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2
12
bis-p-nitrophenyl phosphate
-
mutant enzyme D236A, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
12
bis-p-nitrophenyl phosphate
-
mutant enzyme R237K, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2
18
bis-p-nitrophenyl phosphate
-
mutant enzyme R237A, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2
18
bis-p-nitrophenyl phosphate
-
mutant enzyme R237Q, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2
24
bis-p-nitrophenyl phosphate
-
mutant enzyme H264A, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
26
bis-p-nitrophenyl phosphate
-
mutant enzyme H189Q, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
38
bis-p-nitrophenyl phosphate
-
mutant enzyme H264Q, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
58
bis-p-nitrophenyl phosphate
-
mutant enzyme H189E, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
88
bis-p-nitrophenyl phosphate
-
wild type enzyme, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
1
p-nitrophenyl phosphate
-
mutant enzyme H376N, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2
1.3
p-nitrophenyl phosphate
-
mutant enzyme D392E, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2
4.9
p-nitrophenyl phosphate
-
mutant enzyme D236A, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2
5
p-nitrophenyl phosphate
-
mutant enzyme D236N, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2
6.6
p-nitrophenyl phosphate
-
mutant enzyme D236E, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2
12
p-nitrophenyl phosphate
-
wild type enzyme, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2
13
p-nitrophenyl phosphate
-
mutant enzyme R237K, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2
75
p-nitrophenyl phosphate
-
mutant enzyme H264N, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2
