3.1.3.32: polynucleotide 3'-phosphatase
This is an abbreviated version!
For detailed information about polynucleotide 3'-phosphatase, go to the full flat file.
Word Map on EC 3.1.3.32
-
3.1.3.32
-
3'-phosphate
-
exonuclease
-
5'-kinase
-
ape1
-
5\'-hydroxyl
-
3'-blocking
-
xrcc4
-
end-healing
-
apraxia
-
apurinic
-
aprataxin
-
diesterase
-
end-processing
-
3'-phosphorylated
-
strand-breaks
-
3'-phosphoesterase
-
3'-phosphoglycolate
-
phosphoesterase
-
tyrosyl-dna
-
analysis
-
ap-endonuclease
-
pharmacology
-
medicine
- 3.1.3.32
- 3'-phosphate
-
exonuclease
-
5'-kinase
- ape1
-
5\'-hydroxyl
-
3'-blocking
- xrcc4
-
end-healing
- apraxia
-
apurinic
- aprataxin
- diesterase
-
end-processing
-
3'-phosphorylated
-
strand-breaks
-
3'-phosphoesterase
-
3'-phosphoglycolate
-
phosphoesterase
-
tyrosyl-dna
- analysis
- ap-endonuclease
- pharmacology
- medicine
Reaction
Synonyms
2'(3')-polynucleotidase, 3' phosphatase, 3'-phosphatase, 3'-phosphatase/5'-OH kinase, 5' polynucleotidekinase 3' phosphatase, 5'-polynucleotide kinase/3'-phosphatase, APLF, APTX/PNKP-like factor, AtZDP, chromatin 3'-phosphatase, deoxyribonucleate 3'-phosphatase, DNA 3'-phosphatase, phosphatase, polynucleotide 3'-, Pnk1, PNKP, polynucleotide kinase 3'-phosphatase, polynucleotide kinase phosphatase, polynucleotide kinase-phosphatase, polynucleotide kinase/phosphatase, RNA ligase 1, Rnl1, SNQI-PNK, T4 polynucleotide kinase/phosphatase, Tpp1
ECTree
3.1.3.32 polynucleotide 3'-phosphataseAdvanced search results
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Turnover Number on EC 3.1.3.32 - polynucleotide 3'-phosphatase
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TURNOVER NUMBER [1/s] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
27.83
p-Nitrophenyl phenylphosphonate
-
wild type enzyme, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
additional information
2',3'-cAMP
-
mutant enzyme D236E, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
0.04 - 1.97
2',3'-cAMP
-
mutant enzyme R237A, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
0.04 - 1.97
2',3'-cAMP
-
wild type enzyme, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
0.833
2',3'-cAMP
-
mutant enzyme D392E, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
0.97
2',3'-cAMP
-
mutant enzyme H189E, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
1.1
2',3'-cAMP
-
mutant enzyme D392N, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
1.45
2',3'-cAMP
-
mutant enzyme H189A, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
1.67
2',3'-cAMP
-
mutant enzyme D392A, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
3.2
2',3'-cAMP
-
mutant enzyme H376D, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
3.25
2',3'-cAMP
-
mutant enzyme D233E, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
3.5
2',3'-cAMP
-
mutant enzyme R237Q, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
4.53
2',3'-cAMP
-
mutant enzyme D236A, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
5.13
2',3'-cAMP
-
mutant enzyme H376N, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
5.77
2',3'-cAMP
-
mutant enzyme D236N, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
6.03
2',3'-cAMP
-
mutant enzyme D236E, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
6.5
2',3'-cAMP
-
mutant enzyme H323Q, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
8.1
2',3'-cAMP
-
mutant enzyme R237K, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
8.55
2',3'-cAMP
-
mutant enzyme R237A, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
8.93
2',3'-cAMP
-
wild type enzyme, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
9.1
2',3'-cAMP
-
mutant enzyme H323A, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
15.97
2',3'-cAMP
-
mutant enzyme D392E, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
17.17
2',3'-cAMP
-
mutant enzyme H189E, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
18.67
2',3'-cAMP
-
mutant enzyme D392N, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
20.17
2',3'-cAMP
-
mutant enzyme R237Q, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
26.17
2',3'-cAMP
-
mutant enzyme R237K, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
34.33
2',3'-cAMP
-
mutant enzyme H189D, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2, at 45°C
0.26
bis-p-nitrophenyl phosphate
-
mutant enzyme H264Q, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
0.52
bis-p-nitrophenyl phosphate
-
mutant enzyme D392N, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
0.92
bis-p-nitrophenyl phosphate
-
mutant enzyme H264Q, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2
1.1
bis-p-nitrophenyl phosphate
-
mutant enzyme R237A, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2
1.23
bis-p-nitrophenyl phosphate
-
mutant enzyme H264A, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
1.7
bis-p-nitrophenyl phosphate
-
wild type enzyme, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2
2.13
bis-p-nitrophenyl phosphate
-
mutant enzyme H376D, at 45°C, in 50 mM Tris-HCl (pH 8.0) 0.5 mM MnCl2
2.53
bis-p-nitrophenyl phosphate
-
mutant enzyme D236A, at 45°C, in 50 mM Tris-HCl (pH 8.0) 0.5 mM NiCl2
3 - 6
bis-p-nitrophenyl phosphate
-
mutant enzyme H189D, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
3.13
bis-p-nitrophenyl phosphate
-
mutant enzyme H376N, at 45°C, in 50 mM Tris-HCl (pH 8.0),0.5 mM MnCl2
3.33
bis-p-nitrophenyl phosphate
-
mutant enzyme H189Q, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
3.63
bis-p-nitrophenyl phosphate
-
mutant enzyme R237K, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2
3.72
bis-p-nitrophenyl phosphate
-
mutant enzyme H189A, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
4.05
bis-p-nitrophenyl phosphate
-
mutant enzyme H376N, at 45°C, in 50 mM Tris-HCl (pH 8.0),0.5 mM MnCl2
8.8
bis-p-nitrophenyl phosphate
-
mutant enzyme D392E, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
9.33
bis-p-nitrophenyl phosphate
-
mutant enzyme D236N, at 45°C, in 50 mM Tris-HCl (pH 8.0) 0.5 mM NiCl2
12
bis-p-nitrophenyl phosphate
-
mutant enzyme H264N, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2
13.77
bis-p-nitrophenyl phosphate
-
mutant enzyme D392N, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
16.9
bis-p-nitrophenyl phosphate
-
wild type enzyme, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
18
bis-p-nitrophenyl phosphate
-
mutant enzyme H264A, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2
19.33
bis-p-nitrophenyl phosphate
-
mutant enzyme R237A, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2
23.17
bis-p-nitrophenyl phosphate
-
wild type enzyme, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
33
bis-p-nitrophenyl phosphate
-
mutant enzyme R237Q, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM NiCl2
43.5
bis-p-nitrophenyl phosphate
-
mutant enzyme D236A, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
52.33
bis-p-nitrophenyl phosphate
-
mutant enzyme H264Q, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
65.83
bis-p-nitrophenyl phosphate
-
mutant enzyme D236N, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
117.5
bis-p-nitrophenyl phosphate
-
mutant enzyme H189E, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
253.3
bis-p-nitrophenyl phosphate
-
mutant enzyme H264A, at 45°C, in 50 mM Tris-HCl (pH 8.0), 0.5 mM MnCl2
1.88
p-nitrophenyl phosphate
-
mutant enzyme D236E, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2
2.5
p-nitrophenyl phosphate
-
mutant enzyme R237K, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2
3.42
p-nitrophenyl phosphate
-
mutant enzyme D392E, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2
5.25
p-nitrophenyl phosphate
-
mutant enzyme H376N, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2
9.58
p-nitrophenyl phosphate
-
mutant enzyme D236A, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2
15
p-nitrophenyl phosphate
-
wild type enzyme, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2
30.83
p-nitrophenyl phosphate
-
wild type enzyme, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2
38.33
p-nitrophenyl phosphate
-
mutant enzyme D236N, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2
146.7
p-nitrophenyl phosphate
-
mutant enzyme H264N, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2
