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Literature summary for 3.1.3.32 extracted from

  • Jilani, A.; Ramotar, D.
    Purification and partial characterization of a DNA 3'-phosphatase from Schizosaccharomyces pombe (2002), Biochemistry, 41, 7688-7694.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
NaCl can replace Mg2+ at 50 mM, inhibitory at 250 mM Schizosaccharomyces pombe

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, best at 5-10 mM Schizosaccharomyces pombe
Mn2+ can partly replace Mg2+ Schizosaccharomyces pombe
additional information not stimulating: Cu2+, Co2+, Fe2+, Ca2+ Schizosaccharomyces pombe
NaCl can replace Mg2+ at 50 mM, inhibitory at 250 mM Schizosaccharomyces pombe

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 45000, SDS-PAGE Schizosaccharomyces pombe

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe O13911
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bleomycin-damaged DNA + H2O
-
Schizosaccharomyces pombe ?
-
?
H2O2-damaged DNA + H2O
-
Schizosaccharomyces pombe ?
-
?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Schizosaccharomyces pombe