1.6.1.2: NAD(P)+ transhydrogenase (Re/Si-specific)

This is an abbreviated version!
For detailed information about NAD(P)+ transhydrogenase (Re/Si-specific), go to the full flat file.

Word Map on EC 1.6.1.2

Reaction

NADPH
+
NAD+
=
NADP+
+
NADH

Synonyms

dII, dIII, energy-linked transhydrogenase, energy-linked, mitochondrial transhydrogenase, H+-thase, mitochondrial transhydrogenase, NAD transhydrogenase, NAD(P) transhydrogenase, NADH transhydrogenase, NADH-NADP-transhydrogenase, NADPH transhydrogenase, NADPH-NAD oxidoreductase, NADPH-NAD transhydrogenase, NADPH:NAD+ transhydrogenase, nicotinamide adenine dinucleotide (phosphate) transhydrogenase, nicotinamide nucleotide transhydrogenase, NNT, PNT, PntAB, proton-translocating nicotinamide nucleotide transhydrogenase, proton-translocating transhydrogenase, pyridine nucleotide transferase, pyridine nucleotide transhydrogenase, reversible mitochondrial membrane-associated transhydrogenase, transhydrogenase, transhydrogenase, nicotinamide adenine dinucleotide (phosphate)

ECTree

     1 Oxidoreductases
         1.6 Acting on NADH or NADPH
             1.6.1 With NAD+ or NADP+ as acceptor
                1.6.1.2 NAD(P)+ transhydrogenase (Re/Si-specific)

Purification

Purification on EC 1.6.1.2 - NAD(P)+ transhydrogenase (Re/Si-specific)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography
affinity chromatography on NADP+/NAD+-agarose gels, partial purification
-
affinity chromatography; NaCl wash, Triton X-100 extraction, affinity chromatography on immobilized NAD+
-
FPLC in the presence of 0.05 or 0.1% Triton X-100, comparison of methods
-
fractionation of submitochondrial particles, DEAE-Sepharose, hydroxyapatite
-
immunoexclusion chromatography
-
Ni-NTA column chromatography
Nnt is purified using an immunocapture bead matrix
overview of early procedures
-
partially purified
-
recombinant domain I
-
recombinant domain III
-
recombinant domain III-domain I protein
-
recombinant domains I and III
-
recombinant enzyme
-
recombinant protein
recombinant protein using His-tag
recombinant proteins
-
soluble component
-