1.6.1.2: NAD(P)+ transhydrogenase (Re/Si-specific)

This is an abbreviated version!
For detailed information about NAD(P)+ transhydrogenase (Re/Si-specific), go to the full flat file.

Word Map on EC 1.6.1.2

Reaction

NADPH
+
NAD+
=
NADP+
+
NADH

Synonyms

dII, dIII, energy-linked transhydrogenase, energy-linked, mitochondrial transhydrogenase, H+-thase, mitochondrial transhydrogenase, NAD transhydrogenase, NAD(P) transhydrogenase, NADH transhydrogenase, NADH-NADP-transhydrogenase, NADPH transhydrogenase, NADPH-NAD oxidoreductase, NADPH-NAD transhydrogenase, NADPH:NAD+ transhydrogenase, nicotinamide adenine dinucleotide (phosphate) transhydrogenase, nicotinamide nucleotide transhydrogenase, NNT, PNT, PntAB, proton-translocating nicotinamide nucleotide transhydrogenase, proton-translocating transhydrogenase, pyridine nucleotide transferase, pyridine nucleotide transhydrogenase, reversible mitochondrial membrane-associated transhydrogenase, transhydrogenase, transhydrogenase, nicotinamide adenine dinucleotide (phosphate)

ECTree

     1 Oxidoreductases
         1.6 Acting on NADH or NADPH
             1.6.1 With NAD+ or NADP+ as acceptor
                1.6.1.2 NAD(P)+ transhydrogenase (Re/Si-specific)

General Stability

General Stability on EC 1.6.1.2 - NAD(P)+ transhydrogenase (Re/Si-specific)

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
cations prevent from tryptic inactivation, 95% protection with Mn2+, 89% with Ca2+ and 79% with Mg2+
-
inactivation during prolonged column chromatography
-
solubilized membrane component is unstable loosing all activity when stored overnight at 4C or -70C, stable at -70C in the presence of 0.025 mM NADP+ for several months, inactivation by refreezing after thawing
-
trypsinolysis is stimulated several fold by NADPH and NADP+, half-maximal stimulation with 0.001-0.002 mM NADPH and 0.002-0.003 mM NADP+, Mg2+ protects from NADP+ stimulated inactivation
-
unstable, loses 30-50% activity within 48 h at 4C, remainder of the enzyme becomes inactive after 2 weeks, complete inactivation after freezing at -20C or -70C overnight, enzyme is very susceptible to trypsinolysis, NADH protects from tryptic inactivation, NADPH promotes tryptic inactivation
-
urea, 6 M, inactivation
-