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1.5.1.39: FMN reductase [NAD(P)H]

This is an abbreviated version!
For detailed information about FMN reductase [NAD(P)H], go to the full flat file.

Reaction

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FMNH2
+
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NAD(P)+
=
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FMN
+
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NAD(P)H
+
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H+

Synonyms

ChuY, EC 1.5.1.29, EC 1.6.8.1, flavin mononucleotide reductase, FMN reductase, fre, FRG, H2O-forming FOR, LrFOR, NAD(P)H-flavin reductase, NAD(P)H:FMN-oxidoreductase, NADH: FMN oxidoreductase, NADPH-dependent FMN reductase, NfoR, non-specific NAD(P)H-FMN reductase, pden_5119, Red, SsuE, two-component FMN-dependent monooxygenase, water forming NADH: FMN oxidoreductase

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                EC 1.5.1.391.5.1.39 FMN reductase [NAD(P)H]

Crystallization

Crystallization on EC 1.5.1.39 - FMN reductase [NAD(P)H]

for references in articles please use BRENDA:EC1.5.1.39

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant His-tagged enzyme, using 0.1 M Bis-Tris, pH 6.5, 50 mM CaCl2, and 30% PEG MME 550 as precipitants, ChuY crystals belong to the primitive monoclinic space group P21 with two molecules forming an asymmetric unit, multi-wavelength anomalous dispersion, using a SeMet-labeled crystal at a resolution of 2.4 A
purified enzyme in apoform or in complex with FMN, sitting drop vapor diffusion method, mixing of 0.006 ml of 45 mg/ml protein in 10 mM NAD+, 50 mM HEPES, and 300 mM NaCl, pH 7.5, with 0.006 ml of well solution containing 30% PEG 4000, 210 mM ammonium acetate, and 100 mM sodium citrate, pH 5.6, 14 days, room temperature, X-ray diffraction structure determination and analysis at 2.02 A resolution