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Literature summary for 1.5.1.39 extracted from
- NfoR chromate reductase or flavin mononucleotide reductase? (2020), Appl. Environ. Microbiol., 86, e01758-20 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Staphylococcus aureus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified enzyme in apoform or in complex with FMN, sitting drop vapor diffusion method, mixing of 0.006 ml of 45 mg/ml protein in 10 mM NAD+, 50 mM HEPES, and 300 mM NaCl, pH 7.5, with 0.006 ml of well solution containing 30% PEG 4000, 210 mM ammonium acetate, and 100 mM sodium citrate, pH 5.6, 14 days, room temperature, X-ray diffraction structure determination and analysis at 2.02 A resolution | Staphylococcus aureus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.141 | - |
NADPH | pH 7.5, 25°C | Staphylococcus aureus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| FMN + NADH + H+ | Staphylococcus aureus | - |
FMNH2 + NAD+ | - |
r | |
| FMN + NADPH + H+ | Staphylococcus aureus | - |
FMNH2 + NADP+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | W8TQN2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration | Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| FMN + NADH + H+ | - |
Staphylococcus aureus | FMNH2 + NAD+ | - |
r | |
| FMN + NADPH + H+ | - |
Staphylococcus aureus | FMNH2 + NADP+ | - |
r | |
| additional information | transient-state FMN reduction, overview. Both rapid NADPH binding and hydride transfer to FMN in NfoR. The low relative rate observed is likely a function of the limiting concentration of FMN present or the trapping of the enzyme in an inhibitory product complex (e.g. NfoR-FMNH2-NADPH) in which the release of FMNH2 and/or NADPH is slow. Reoxidation of flavins (FADH2) by chromate and copper | Staphylococcus aureus | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FMN reductase | - |
Staphylococcus aureus |
| NfoR | - |
Staphylococcus aureus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Staphylococcus aureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Staphylococcus aureus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Staphylococcus aureus | |
| NADH | - |
Staphylococcus aureus | |
| NADP+ | - |
Staphylococcus aureus | |
| NADPH | - |
Staphylococcus aureus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | NfoR, an enzyme claimed to be a chromate reductase, is in fact an FMN reductase | Staphylococcus aureus |
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Release 2023.1 (January 2023)
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