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show all sequences of 1.5.1.39

Structural and functional study of ChuY from Escherichia coli strain CFT073

Kim, H.; Chaurasia, A.; Kim, T.; Choi, J.; Ha, S.; Kim, D.; Kim, K.; Biochem. Biophys. Res. Commun. 482, 1176-1182 (2017)

Data extracted from this reference:

Application
Application
Commentary
Organism
drug development
enzyme ChuY is a potential target for the development of antibacterial or antivirulence drugs to combat not only UPEC but a broad range of pathogenic bacteria that contain chuY system or its orthologue, ranging from Vibrio to staphylococcal species
Escherichia coli
Cloned(Commentary)
Commentary
Organism
gene chuY, encoded in the chu gene cluster of strain CFT073, recombinant expression as His-tagged enzyme in Escherichia coli, complementation of an Escherichia coli chuY-knockout strain with expression of the wild-type gene
Escherichia coli
Crystallization (Commentary)
Crystallization
Organism
purified recombinant His-tagged enzyme, using 0.1 M Bis-Tris, pH 6.5, 50 mM CaCl2, and 30% PEG MME 550 as precipitants, ChuY crystals belong to the primitive monoclinic space group P21 with two molecules forming an asymmetric unit, multi-wavelength anomalous dispersion, using a SeMet-labeled crystal at a resolution of 2.4 A
Escherichia coli
Engineering
Amino acid exchange
Commentary
Organism
additional information
construction of a chuY-knockout strain
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
FMNH2 + NAD(P)+
Escherichia coli
-
FMN + NAD(P)H + H+
-
-
r
FMNH2 + NAD(P)+
Escherichia coli CFT073
-
FMN + NAD(P)H + H+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
A0A0H2VDD2
a non-infectious strain
-
Escherichia coli CFT073
A0A0H2VDD2
a non-infectious strain
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli by metal affinity chromatography and gel filtration
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
FMNH2 + NAD(P)+
-
741831
Escherichia coli
FMN + NAD(P)H + H+
-
-
-
r
FMNH2 + NAD(P)+
-
741831
Escherichia coli CFT073
FMN + NAD(P)H + H+
-
-
-
r
Subunits
Subunits
Commentary
Organism
monomer
ChuY exists as a monomer in solution, SDS-PAGE and gel filtration
Escherichia coli
More
the two molecules in the asymmetric unit are related by pseudo 2fold rotation symmetry. ChuY contains six alpha-helices and ten beta-strands. A central beta-sheet, consisting of seven parallel beta-strands, beta1, beta2, beta3, beta4, beta5, beta6, and beta10, is flanked by six alpha-helices, forming alternating beta-strand and alpha-helix repeats, which is a representative feature of Rossmann folds. Three other beta-strands (beta7-beta9) are located on the top of the beta-sheet
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
heme
the enzyme has a hemin binding activity, pronbably in the hydrophobic pocket near loops beta4-alpha5 and beta8-beta9, docking of biliverdin IXalpha and FMN , overview
Escherichia coli
additional information
enzyme ChuY binds nucleotides as cofactors., cofactor binding structure, overview
Escherichia coli
NAD+
-
Escherichia coli
NADH
-
Escherichia coli
NADP+
-
Escherichia coli
NADPH
-
Escherichia coli
Application (protein specific)
Application
Commentary
Organism
drug development
enzyme ChuY is a potential target for the development of antibacterial or antivirulence drugs to combat not only UPEC but a broad range of pathogenic bacteria that contain chuY system or its orthologue, ranging from Vibrio to staphylococcal species
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
gene chuY, encoded in the chu gene cluster of strain CFT073, recombinant expression as His-tagged enzyme in Escherichia coli, complementation of an Escherichia coli chuY-knockout strain with expression of the wild-type gene
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
heme
the enzyme has a hemin binding activity, pronbably in the hydrophobic pocket near loops beta4-alpha5 and beta8-beta9, docking of biliverdin IXalpha and FMN , overview
Escherichia coli
additional information
enzyme ChuY binds nucleotides as cofactors., cofactor binding structure, overview
Escherichia coli
NAD+
-
Escherichia coli
NADH
-
Escherichia coli
NADP+
-
Escherichia coli
NADPH
-
Escherichia coli
Crystallization (Commentary) (protein specific)
Crystallization
Organism
purified recombinant His-tagged enzyme, using 0.1 M Bis-Tris, pH 6.5, 50 mM CaCl2, and 30% PEG MME 550 as precipitants, ChuY crystals belong to the primitive monoclinic space group P21 with two molecules forming an asymmetric unit, multi-wavelength anomalous dispersion, using a SeMet-labeled crystal at a resolution of 2.4 A
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction of a chuY-knockout strain
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
FMNH2 + NAD(P)+
Escherichia coli
-
FMN + NAD(P)H + H+
-
-
r
FMNH2 + NAD(P)+
Escherichia coli CFT073
-
FMN + NAD(P)H + H+
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged enzyme from Escherichia coli by metal affinity chromatography and gel filtration
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
FMNH2 + NAD(P)+
-
741831
Escherichia coli
FMN + NAD(P)H + H+
-
-
-
r
FMNH2 + NAD(P)+
-
741831
Escherichia coli CFT073
FMN + NAD(P)H + H+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
ChuY exists as a monomer in solution, SDS-PAGE and gel filtration
Escherichia coli
More
the two molecules in the asymmetric unit are related by pseudo 2fold rotation symmetry. ChuY contains six alpha-helices and ten beta-strands. A central beta-sheet, consisting of seven parallel beta-strands, beta1, beta2, beta3, beta4, beta5, beta6, and beta10, is flanked by six alpha-helices, forming alternating beta-strand and alpha-helix repeats, which is a representative feature of Rossmann folds. Three other beta-strands (beta7-beta9) are located on the top of the beta-sheet
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Escherichia coli
General Information
General Information
Commentary
Organism
evolution
gene chuY is highly conserved in a broad range of pathogenic bacteria
Escherichia coli
malfunction
a chuY deletion-insertion strain shows reduced survival potential compared to wild-type and complemented strains in mammalian cells
Escherichia coli
physiological function
ChuY has flavin mononucleotide (FMN) reductase activity, using NAD(P)H as a cofactor, and shows porphyrin ring binding affinity. ChuY acts as a reductase in heme homeostasis to maintain the virulence potential of Escherichia coli strain CFT073
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
evolution
gene chuY is highly conserved in a broad range of pathogenic bacteria
Escherichia coli
malfunction
a chuY deletion-insertion strain shows reduced survival potential compared to wild-type and complemented strains in mammalian cells
Escherichia coli
physiological function
ChuY has flavin mononucleotide (FMN) reductase activity, using NAD(P)H as a cofactor, and shows porphyrin ring binding affinity. ChuY acts as a reductase in heme homeostasis to maintain the virulence potential of Escherichia coli strain CFT073
Escherichia coli
Other publictions for EC 1.5.1.39
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741831
Kim
Structural and functional stu ...
Escherichia coli, Escherichia coli CFT073
Biochem. Biophys. Res. Commun.
482
1176-1182
2017
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716204
Watanabe
Specificities and properties o ...
Vibrio harveyi, Vibrio harveyi MB-20
Mol. Cell. Biochem.
44
181-187
1982
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