1.5.1.11: D-octopine dehydrogenase

This is an abbreviated version!
For detailed information about D-octopine dehydrogenase, go to the full flat file.

Word Map on EC 1.5.1.11

Reaction

N2-(D-1-carboxyethyl)-L-arginine
+
NAD+
+
H2O
=
L-arginine
+
pyruvate
+
NADH
+
H+

Synonyms

OCS, D-Octopine synthase, Octopine dehydrogenase, Dehydrogenase, octopine, Octopine:NAD oxidoreductase, ODH, Octopine synthase, N2-(D-1-carboxyethyl)-L-arginine:NAD+ oxidoreductase, OcDH, N2-(D-1-carboxyethyl)-L-arginine:NAD+-oxidoreductase, flavin-containing opine dehydrogenase, pTi_008

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.11 D-octopine dehydrogenase

Purification

Purification on EC 1.5.1.11 - D-octopine dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 isoenzymes: A and B
-
ammonium sulfate precipitation, Ni2+-NTA column chromatography, and Sephadex G-100 gel filtration
crown-gall tumor-tissue induced by Agrobacterium tumefaciens
glutathione Sepharose column chromatography
-
His5-tagged OcDH and His6-tagged OcDH are purified by Ni-NTA chromatography, yielding almost 20 mg homogenous enzyme per litre of cell culture (purity greater than 98%). The purification of OcDH-tagless requires several chromatographic steps and yields 3-5 mg per litre of cell culture, with a purity of more than 98%
Ni-NTA affinity chromatography and Sephadex G-100 gel filtration
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, dialysis, and gel filtration
-
the biospecific chromatographic affinity purification is not applicable to the purification of the enzyme from Monodonta lineate
-
the biospecific chromatographic affinity purification is not applicable to the purification of the enzyme from Mytilus edulis
-