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Literature summary for 1.5.1.11 extracted from

  • Vazquez-Dorado, S.; Sanjuan, A.; Comesana, A.S.; de Carlos, A.
    Identification of octopine dehydrogenase from Mytilus galloprovincialis (2011), Comp. Biochem. Physiol. B, 160, 94-103.
    View publication on PubMed
Search for more literature for 1.5.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Mytilus galloprovincialis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
 NADH GST-tagged enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
0.026
-
 NADH crude extract enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
0.028
-
 NADH recombinant enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
0.25
-
 pyruvate crude extract enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
0.35
-
 pyruvate GST-tagged enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
0.35
-
 pyruvate recombinant enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
1.4
-
 L-lysine GST-tagged enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
1.8
-
 L-lysine crude extract enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
1.8
-
 L-lysine recombinant enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44300
-
 x * 44300, calculated from amino acid sequence Mytilus galloprovincialis
70000
-
 gel filtration Mytilus galloprovincialis

Organism

Organism UniProt Comment Textmining
Mytilus galloprovincialis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography Mytilus galloprovincialis

Source Tissue

Source Tissue Comment Organism Textmining
digestive gland
-
 Mytilus galloprovincialis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.45
-
 crude extract enzyme, using beta-alanine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
1.5
-
 crude extract enzyme, using L-alanine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
1.7
-
 crude extract enzyme, using glycine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
10.1
-
 recombinant enzyme, using glycine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
10.4
-
 crude extract enzyme, using L-arginine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
33.3
-
 recombinant enzyme, using L-alanine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
324.8
-
 recombinant enzyme, using L-arginine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-alanine + pyruvate + NADH + H+
-
 Mytilus galloprovincialis ?
-
 ?
glycine + pyruvate + NADH + H+
-
 Mytilus galloprovincialis ?
-
 ?
L-alanine + pyruvate + NADH + H+
-
 Mytilus galloprovincialis ?
-
 ?
L-arginine + pyruvate + NADH + H+ highest affinity for L-arginine Mytilus galloprovincialis N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O
-
 ?
L-lysine + pyruvate + NADH + H+
-
 Mytilus galloprovincialis ?
-
 ?
additional information the enzyme shows no activity when taurine or beta-alanine is used as substrate Mytilus galloprovincialis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 44300, calculated from amino acid sequence Mytilus galloprovincialis
? x * 70000, GST-tagged enzyme, SDS-PAGE Mytilus galloprovincialis

Synonyms

Synonyms Comment Organism
OcDH
-
 Mytilus galloprovincialis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
 Mytilus galloprovincialis

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Mytilus galloprovincialis

pI Value

Organism Comment pI Value Maximum pI Value
Mytilus galloprovincialis calculated from amino acid sequence
-
 5.9