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1.5.1.11: D-octopine dehydrogenase

This is an abbreviated version!
For detailed information about D-octopine dehydrogenase, go to the full flat file.

Word Map on EC 1.5.1.11

Reaction

N2-(D-1-carboxyethyl)-L-arginine
+
NAD+
+
H2O
=
L-arginine
+
pyruvate
+
NADH
+
H+

Synonyms

D-Octopine synthase, Dehydrogenase, octopine, flavin-containing opine dehydrogenase, N2-(D-1-carboxyethyl)-L-arginine:NAD+ oxidoreductase, N2-(D-1-carboxyethyl)-L-arginine:NAD+-oxidoreductase, OcDH, OCS, Octopine dehydrogenase, Octopine synthase, Octopine:NAD oxidoreductase, ODH, pTi_008

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.11 D-octopine dehydrogenase

Cloned

Cloned on EC 1.5.1.11 - D-octopine dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli ER2566 cells
expressed in Escherichia coli strain ER2566
-
expressed in Escherichia coli with a C-terminal penta His-tag
gene ocs, DNA and amino acid sequence determination, analysis, and comparisons, genetic structure, phylogenetic analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
OcDH is cloned and expressed tagless (OcDH-tagless), as a His5-tagged OcDH (OcDH-His5) and as a His6-tagged OcDH variant (OcDHLEHis6) in Escherichia coli. The His tag is always placed at the C-terminus